ISfinder Mirror Site

ISBfun4

Family IS701
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007953	ND	Burkholderia xenovorans	Burkholderia xenovorans LB400

DNA section

IS Length : 1464 bp

Ends

IR Length : 19/20

IRL : TACTACTGTGTCATAAAATTCATGTCATTATAGGAGCTTCTTCATCCTTT
IRR : TACTACTGTGTCATAAAGTTATCTGAAGTAGCGCAGCAAAACGGACATCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AACCGGACAC		CTACTTGTCT	0

DNA sequence

TACTACTGTGTCATAAAATTCATGTCATTATAGGAGCTTCTTCATCCTTTACATGAGGAAGTGATGAACTGGGAGACTTCGTTCGACGAGTACCTGGAAC
ACCTATGCAAGGTAGTCGGGCACAGTGATCGCCGCGCCGGGCTGACTGGCTATTGCAGGGGGCTGATGCGGCCACTCGGGCGCAAGAGCGTCGAGCCGTT
GGCAGCCCATCTGGAGCCGCATCGCGTAAGCGCGCGGCACCAGTCGCTGCATCACTTCGTGTCAAAGTCGGAATGGTCTGACGCCGCGCTACTCGAACAG
GTGCGCCGCTGGGTGTTGCCGCACATGGACCCGGCAAAGGGCCTGTACTGGATGATTGATGACACGGGCTTCCCGAAGAAAGGCAGGCATTCGGTCGGCG
TAGCGCGGCAATACTGCGGGCAGTTGGGCAAACAGGATAACTGCCAGGTTGCCGTGAGTCTGTCCGTAGCCACCGAAGCGGCGAGCCTGCCCGTCGCTTA
CCAGCTTTATCTCCCACAGGAATGGACCGACGATCCGACAAGACGGCAGCAGGCCGGCGTGCCCGATGAGATCGGGTTTGCGACCAAGCCCCAGATCGCG
GTCACGCAGTTGCGCGAGGCACGGCAAAGCGGTGCCCCGGACGGGGTTGTACTGGCCGATGCGGGTTACGGAAACGACACCGCGTTTCGCGATGCCCTTG
GCGAACTCGGCCTGCAATATGCGGTGGGTATCCAGTCGGGCACCCGCGTGTGGCCACCAGGCCTCGCCCCGCTGCCAGCCGAATCCTCGCAGGGCAAGGC
TGGCCGGCCGCGAAGCCTGCAGCGCCGCATACCAGGACATGATCCCGTCCCGGTCAAGGACCTGGCGCTGGCACCCGAACCATCCCGCTACCACACGGCT
TCATGGCGCGAAGGCACGCGTGCCACACTCAGCTCGCGATTTGCGGCTGTTCGGGTTCGCGCCTCACACCGCGATTACTGGCGTGCTACCCCACGCGACG
AAGAATGGCTGCTGATCGAGTGGCCCAACGGGGAGTCTGAGCCAACCAAATACTGGCTTTCAACATTGCCCGATGACACACCAGTAGAACGTCTGGCTTA
CGTTGCAAAAATGCGCTGGCGCACCGAGCGCGACTACCAGGATCTCAAGCAGGAGTTCGGCATTGGCCACTTCGAGGGACGAGGATGGCGCGGGTTTCAT
CACCATGCATCGCTGTGCATTGCCGCTTATGGGTTCCTTGTCGCACAACGCCTCATTCAAGGGGGCAGTAAAAAAAACGCCGAAATCCGCAACCCGGTTG
CCTTACCCGCGGACTACGTCCCACGCGGCTCCCCAGCGCGCGCAGCGACACGTACCTGATTCGATCGCAACGTTGCGCTGGTGCATTGCTGTCCACATCT
CCAGGCAGCTGCAGCGATGTCCGTTTTGCTGCGCTACTTCAGATAACTTTATGACACAGTAGTA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1293 bp	431 aa	64	1356	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNWETSFDEYLEHLCKVVGHSDRRAGLTGYCRGLMRPLGRKSVEPLAAHLEPHRVSARHQSLHHFVSKSEWSDAALLEQVRRWVLPHMDPAKGLYWMIDD
TGFPKKGRHSVGVARQYCGQLGKQDNCQVAVSLSVATEAASLPVAYQLYLPQEWTDDPTRRQQAGVPDEIGFATKPQIAVTQLREARQSGAPDGVVLADA
GYGNDTAFRDALGELGLQYAVGIQSGTRVWPPGLAPLPAESSQGKAGRPRSLQRRIPGHDPVPVKDLALAPEPSRYHTASWREGTRATLSSRFAAVRVRA
SHRDYWRATPRDEEWLLIEWPNGESEPTKYWLSTLPDDTPVERLAYVAKMRWRTERDYQDLKQEFGIGHFEGRGWRGFHHHASLCIAAYGFLVAQRLIQG
GSKKNAEIRNPVALPADYVPRGSPARAATRT

Blast result :

Comments

ISBfun4 is 79% aa similar to ISRso17. ISBfun4 was found by screening completely sequenced genomes for sequences homologous to the IS701 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-99-D(N3)-155-E(C1). The copy number in Burkholderia xenovorans LB400 is 1 (on chromosome 3).

References

1]De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2]Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Lykidis,A. and Richardson,P.(2006)Unpublished