ISRba2
- Family IS4
- Group IS50
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005027 | ND | Rhodopirellula baltica | Rhodopirellula baltica SH 1 |
DNA section
IS Length : 1365 bp
Ends
IR Length : 16
IRL : CATTGGTGTCTACACATCTGCTTGACTTTGGTACGAGTCGCTGGATGGGA
IRR : CATTGGTGTCTACACAAGCCGAACCTCACTGAGCGCGTCTTTTTCGAGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACTTCACCCT | CCCTCTGGG | AGGGTCGGCC | 9 |
DNA sequence
CATTGGTGTCTACACATCTGCTTGACTTTGGTACGAGTCGCTGGATGGGATAAAAATAGCGTTGGAAACGCGTTTGCAGAAGCAATATCACGCTCAGGTG
AAGTCGCATATGAAAGCCTCCACGAGGACTAGCCATGGGCCTCGTTGCTCGCTGGACGTGGAGAAATCCACCTCAGCAAGCCAAAACCTCTGGCGATTCC
TCAAAACGAACGAGTTTCGCTCCTAAAGCTCATCGAGCCGCTGCGACAGCTCGGTCGAGACGGCTGCGCGGGCAGTAAGTCGGATTTCGTGCTGCTGGCA
CATGATTGGTGCAAGCTAGCGTACGGTTCGCAGACCAAACGCAAGAAAGATCTGCTGCAGCTCACTCACGAGACGGATATCGGTTACGACCTCACGACCT
CGCTGCTGATCGAAGCCGACACGGGCGTCACTTTGGCACCAATGCAGGTCCACGTCAAAACCGGCGATGCCGTCCATAGCACCGCCGAGCATCCGCCAGC
CCACGATGATCACCACCTCGATCAACTCGAGCCGACAATGGATGAGGTCGCACAGTGGGAGTTGCCTCGGCGTGTCGTTCATGTGATCGATCGCGAAGCC
GACTCACTGGGCCGACTGCGAAGCTGGCACGCCAAAGGGCACTTGTTTCTGGTCCGCTGTGATGATCGCCGAGTCCGCTGTGAAGGCCGATCGGTTCTGT
TGTCAGAACTCAATGACGAGCTCGACAGTCAGTGCGAATACGCCGATGCTGGCAAAGCTCTCTATCATGGAAAGAAGGTTCAGCGTCAGGTTGCCGAGAA
AACCGTCACGCTTTACCGTCCTCATAGCGAGGTGATTGACGGCGAGAAGAAGGCTGTCACTGGCGAGCCTATCGAAGTGCGAACGGTGTTTGTCCGCTTA
GTCGACGCGGACGGCTGGATCCTTGCCGAGTGGACTTTGCTTACAAACGTGCCCGCTGATCAAGCGAATGCATCGGACGTGGGCCGTTGGTACTACTTTC
GTTGGCGGATTGAAAGCTTTTTCAAACTGCTCAAGAGTCACGGCCAGGAGCTGGAATACTGGCAGCAGGAATCTGGTGAGGCGATTACCAAGCGTTTGCT
GATGGCGTCGATGGCATGCGTGCTAGTGAAACAACTCGAAGCGAGCGAGAGTGAATCAACAATCAAATTCCGCCGCCATTTAATACGTCTCAGCGGACGC
CGGATGAAACGGGGCGTCAAGTTCACCGGTCCGGCGTTGCTTGCTGGCTATGGAATCCACTTGACGATGCTCGACTTCCTGATCGAAACGGAAATGACCA
CCGACGAACTGAGGCAACTCGAAAAAGACGCGCTCAGTGAGGTTCGGCTTGTGTAGACACCAATG
AAGTCGCATATGAAAGCCTCCACGAGGACTAGCCATGGGCCTCGTTGCTCGCTGGACGTGGAGAAATCCACCTCAGCAAGCCAAAACCTCTGGCGATTCC
TCAAAACGAACGAGTTTCGCTCCTAAAGCTCATCGAGCCGCTGCGACAGCTCGGTCGAGACGGCTGCGCGGGCAGTAAGTCGGATTTCGTGCTGCTGGCA
CATGATTGGTGCAAGCTAGCGTACGGTTCGCAGACCAAACGCAAGAAAGATCTGCTGCAGCTCACTCACGAGACGGATATCGGTTACGACCTCACGACCT
CGCTGCTGATCGAAGCCGACACGGGCGTCACTTTGGCACCAATGCAGGTCCACGTCAAAACCGGCGATGCCGTCCATAGCACCGCCGAGCATCCGCCAGC
CCACGATGATCACCACCTCGATCAACTCGAGCCGACAATGGATGAGGTCGCACAGTGGGAGTTGCCTCGGCGTGTCGTTCATGTGATCGATCGCGAAGCC
GACTCACTGGGCCGACTGCGAAGCTGGCACGCCAAAGGGCACTTGTTTCTGGTCCGCTGTGATGATCGCCGAGTCCGCTGTGAAGGCCGATCGGTTCTGT
TGTCAGAACTCAATGACGAGCTCGACAGTCAGTGCGAATACGCCGATGCTGGCAAAGCTCTCTATCATGGAAAGAAGGTTCAGCGTCAGGTTGCCGAGAA
AACCGTCACGCTTTACCGTCCTCATAGCGAGGTGATTGACGGCGAGAAGAAGGCTGTCACTGGCGAGCCTATCGAAGTGCGAACGGTGTTTGTCCGCTTA
GTCGACGCGGACGGCTGGATCCTTGCCGAGTGGACTTTGCTTACAAACGTGCCCGCTGATCAAGCGAATGCATCGGACGTGGGCCGTTGGTACTACTTTC
GTTGGCGGATTGAAAGCTTTTTCAAACTGCTCAAGAGTCACGGCCAGGAGCTGGAATACTGGCAGCAGGAATCTGGTGAGGCGATTACCAAGCGTTTGCT
GATGGCGTCGATGGCATGCGTGCTAGTGAAACAACTCGAAGCGAGCGAGAGTGAATCAACAATCAAATTCCGCCGCCATTTAATACGTCTCAGCGGACGC
CGGATGAAACGGGGCGTCAAGTTCACCGGTCCGGCGTTGCTTGCTGGCTATGGAATCCACTTGACGATGCTCGACTTCCTGATCGAAACGGAAATGACCA
CCGACGAACTGAGGCAACTCGAAAAAGACGCGCTCAGTGAGGTTCGGCTTGTGTAGACACCAATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1209 bp | 403 aa | 145 | 1353 | + | No |
Chemistry : DDE
ORF sequence :
MLAGRGEIHLSKPKPLAIPQNERVSLLKLIEPLRQLGRDGCAGSKSDFVLLAHDWCKLAYGSQTKRKKDLLQLTHETDIGYDLTTSLLIEADTGVTLAPM
QVHVKTGDAVHSTAEHPPAHDDHHLDQLEPTMDEVAQWELPRRVVHVIDREADSLGRLRSWHAKGHLFLVRCDDRRVRCEGRSVLLSELNDELDSQCEYA
DAGKALYHGKKVQRQVAEKTVTLYRPHSEVIDGEKKAVTGEPIEVRTVFVRLVDADGWILAEWTLLTNVPADQANASDVGRWYYFRWRIESFFKLLKSHG
QELEYWQQESGEAITKRLLMASMACVLVKQLEASESESTIKFRRHLIRLSGRRMKRGVKFTGPALLAGYGIHLTMLDFLIETEMTTDELRQLEKDALSEV
RLV
QVHVKTGDAVHSTAEHPPAHDDHHLDQLEPTMDEVAQWELPRRVVHVIDREADSLGRLRSWHAKGHLFLVRCDDRRVRCEGRSVLLSELNDELDSQCEYA
DAGKALYHGKKVQRQVAEKTVTLYRPHSEVIDGEKKAVTGEPIEVRTVFVRLVDADGWILAEWTLLTNVPADQANASDVGRWYYFRWRIESFFKLLKSHG
QELEYWQQESGEAITKRLLMASMACVLVKQLEASESESTIKFRRHLIRLSGRRMKRGVKFTGPALLAGYGIHLTMLDFLIETEMTTDELRQLEKDALSEV
RLV
Blast result :
Comments
ISRba2 is 44% aa similar to ISPlu9.
ISRba2 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-94-D(N3)-140-E(C1). The copy number in Rhodopirellula baltica SH 1 is 1.
ISRba2 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-94-D(N3)-140-E(C1). The copy number in Rhodopirellula baltica SH 1 is 1.
References
1] Gloeckner,F.O., Kube,M., Bauer,M., Teeling,H., Lombardot,T., Ludwig,W., Gade,D., Beck,A., Borzym,K., Heitmann,K., Rabus,R., Schlesner,H., Amann,R. and Reinhardt,R. (2003) Proc. Natl. Acad. Sci. U.S.A. 100(14), 8298-8303.
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18