ISGvi2
- Family IS4
- Group IS50
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005125 | ND | Gloeobacter violaceus | Gloeobacter violaceus PCC 7421 |
DNA section
IS Length : 1487 bp
Ends
IR Length : 18/20
IRL : CTATCCATTACCCACATTTTTGAGAGCGCCTCTGTCTGCTGTGCGCCAGT
IRR : CTATCCGTTACCCACATCTTCGTTCCCATCGCCCGCCATCGCTGAGCGGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCACCCCCT | CGACGCGG | GGAGGGGGAG | 8 |
| GGTATCCTCT | CCGCTTTGG | GGGGGGAATG | 9 |
DNA sequence
CTATCCATTACCCACATTTTTGAGAGCGCCTCTGTCTGCTGTGCGCCAGTCACACTGGGAGCGTACTGGCTCAGCCGTGGCCCATGCCGACCATCGACCC
CTGGACCGAACACGAACTGCAACACCTCGATTTGGGCGATGCCCGTCGCCACACCCGACTCAAGCAGCTGCTCTCCAGCCTCGCCCGTCAACCCCATGCC
TCTCTGCCCCAAGCCTGCGAGGATGCCGCCGCCCTCAAAGCCGCCTACCGCTTTTTGGACAACCCGCAGTGCCACCCTGCAGATATCCGCCAGGCCCATG
CCAGTGCCACCGCCGGCCGCCTGGCTGCCCTACCGCTGGTGCTGCTGATTCAAGACACCACCGAGTTGAACTTCACTGCCCACCCCCACACCACCGGGCT
GGGCCATCTGTCCAAGCCCGAAAGCCAGGGACTGCTGGTCCACTCGCTTCTGGCTGTGCGGCCCGACGCCGTCGCCCTCGGCTTGTGCTGGCAGAAAGTC
TGGACCCGTGCGGCCCAGGTGGAGCATCTGGCCGCTCGACGCCAAAAGCGTCCCCAACAGCACAAAGAATCCCAACGCTGGCTGGACGGTCTGGCGGCTG
CCAAAGGCTATGTGCCGCCGGGGGGCGAAGCGGTGCTCATCGGCGACCGCGAGTCGGACATGTTCGGCTTGTTTGCGGCACCGCGCCCGCCGCAGTTGCA
TCTCTTGGTGCGCGCCGCGCGCCAACGTCGGCTGGCCACCCCCAAAACGTTGCTGTTCGAGGTTTTCGCGGGTGCCTCAGCCCAGGGCCACTATCGGTGC
GACCTGGCGCGCCGTCCCGGGCGGGCGGCGCGCCAGGCGAGGTTGCAGGTGTACTGGCAAGCAGTGCAGTTGCAGCCGGGTCGCAATGACCAGACGCACA
AGGGTCAACCGCCGGTGTCGGTGTGGGTGGTGCGCGCCTGGGAAGTGGACCCGCCTGCGGGCGAAGAGGGTATCGATTGGTGGTTGTTGAGTAGCCAGCC
GGTGACCACGCTGGAGCAGGCGCTGGCCTGTGTGCACCGGTACACGCTGCGCTGGTTAATCGAGCGCTATCACTACATTCTCAAAAGTGGTTGCGCGGTG
GAGCAGTTGCAGTTGGAGACGGCACAGCGTTTGGAGCGTGCGTTGGCAGTCTATTGCATCGTGGCGTGGCGGTTGTTGCAATTGACTTACCAGTCTCGCA
GCGAGCCGGAGTTGCCGTGCACGGTGGCGTTGGCGCAGCACGAGTGGCAGGCGTTGTATTGTCGGATGACCCAGAGTCGGCAGTTGCCCGAGGAGGCGCC
GAGTTTACGTCAGGCGGTGCGGTGGATAGCGCAGTTGGGAGGCTTTGTCGGTCGTAAAGGGGACGGTGAGCCTGGGGTGAAGACGCTGTGGCGAGGCTGG
CAGCGGCTGGAGGATTTGGCAGCAGGGTGGCTCATCGCCCGCTCAGCGATGGCGGGCGATGGGAACGAAGATGTGGGTAACGGATAG
CTGGACCGAACACGAACTGCAACACCTCGATTTGGGCGATGCCCGTCGCCACACCCGACTCAAGCAGCTGCTCTCCAGCCTCGCCCGTCAACCCCATGCC
TCTCTGCCCCAAGCCTGCGAGGATGCCGCCGCCCTCAAAGCCGCCTACCGCTTTTTGGACAACCCGCAGTGCCACCCTGCAGATATCCGCCAGGCCCATG
CCAGTGCCACCGCCGGCCGCCTGGCTGCCCTACCGCTGGTGCTGCTGATTCAAGACACCACCGAGTTGAACTTCACTGCCCACCCCCACACCACCGGGCT
GGGCCATCTGTCCAAGCCCGAAAGCCAGGGACTGCTGGTCCACTCGCTTCTGGCTGTGCGGCCCGACGCCGTCGCCCTCGGCTTGTGCTGGCAGAAAGTC
TGGACCCGTGCGGCCCAGGTGGAGCATCTGGCCGCTCGACGCCAAAAGCGTCCCCAACAGCACAAAGAATCCCAACGCTGGCTGGACGGTCTGGCGGCTG
CCAAAGGCTATGTGCCGCCGGGGGGCGAAGCGGTGCTCATCGGCGACCGCGAGTCGGACATGTTCGGCTTGTTTGCGGCACCGCGCCCGCCGCAGTTGCA
TCTCTTGGTGCGCGCCGCGCGCCAACGTCGGCTGGCCACCCCCAAAACGTTGCTGTTCGAGGTTTTCGCGGGTGCCTCAGCCCAGGGCCACTATCGGTGC
GACCTGGCGCGCCGTCCCGGGCGGGCGGCGCGCCAGGCGAGGTTGCAGGTGTACTGGCAAGCAGTGCAGTTGCAGCCGGGTCGCAATGACCAGACGCACA
AGGGTCAACCGCCGGTGTCGGTGTGGGTGGTGCGCGCCTGGGAAGTGGACCCGCCTGCGGGCGAAGAGGGTATCGATTGGTGGTTGTTGAGTAGCCAGCC
GGTGACCACGCTGGAGCAGGCGCTGGCCTGTGTGCACCGGTACACGCTGCGCTGGTTAATCGAGCGCTATCACTACATTCTCAAAAGTGGTTGCGCGGTG
GAGCAGTTGCAGTTGGAGACGGCACAGCGTTTGGAGCGTGCGTTGGCAGTCTATTGCATCGTGGCGTGGCGGTTGTTGCAATTGACTTACCAGTCTCGCA
GCGAGCCGGAGTTGCCGTGCACGGTGGCGTTGGCGCAGCACGAGTGGCAGGCGTTGTATTGTCGGATGACCCAGAGTCGGCAGTTGCCCGAGGAGGCGCC
GAGTTTACGTCAGGCGGTGCGGTGGATAGCGCAGTTGGGAGGCTTTGTCGGTCGTAAAGGGGACGGTGAGCCTGGGGTGAAGACGCTGTGGCGAGGCTGG
CAGCGGCTGGAGGATTTGGCAGCAGGGTGGCTCATCGCCCGCTCAGCGATGGCGGGCGATGGGAACGAAGATGTGGGTAACGGATAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1401 bp | 467 aa | 84 | 1484 | + | No |
Chemistry : DDE
ORF sequence :
MPTIDPWTEHELQHLDLGDARRHTRLKQLLSSLARQPHASLPQACEDAAALKAAYRFLDNPQCHPADIRQAHASATAGRLAALPLVLLIQDTTELNFTAH
PHTTGLGHLSKPESQGLLVHSLLAVRPDAVALGLCWQKVWTRAAQVEHLAARRQKRPQQHKESQRWLDGLAAAKGYVPPGGEAVLIGDRESDMFGLFAAP
RPPQLHLLVRAARQRRLATPKTLLFEVFAGASAQGHYRCDLARRPGRAARQARLQVYWQAVQLQPGRNDQTHKGQPPVSVWVVRAWEVDPPAGEEGIDWW
LLSSQPVTTLEQALACVHRYTLRWLIERYHYILKSGCAVEQLQLETAQRLERALAVYCIVAWRLLQLTYQSRSEPELPCTVALAQHEWQALYCRMTQSRQ
LPEEAPSLRQAVRWIAQLGGFVGRKGDGEPGVKTLWRGWQRLEDLAAGWLIARSAMAGDGNEDVGNG
PHTTGLGHLSKPESQGLLVHSLLAVRPDAVALGLCWQKVWTRAAQVEHLAARRQKRPQQHKESQRWLDGLAAAKGYVPPGGEAVLIGDRESDMFGLFAAP
RPPQLHLLVRAARQRRLATPKTLLFEVFAGASAQGHYRCDLARRPGRAARQARLQVYWQAVQLQPGRNDQTHKGQPPVSVWVVRAWEVDPPAGEEGIDWW
LLSSQPVTTLEQALACVHRYTLRWLIERYHYILKSGCAVEQLQLETAQRLERALAVYCIVAWRLLQLTYQSRSEPELPCTVALAQHEWQALYCRMTQSRQ
LPEEAPSLRQAVRWIAQLGGFVGRKGDGEPGVKTLWRGWQRLEDLAAGWLIARSAMAGDGNEDVGNG
Blast result :
Comments
ISGvi2 is 51% aa similar to ISEcl13.
ISGvi2 was found by screening completely sequenced genomes for sequences homologous to the IS50R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-96-D(N3)-138-E(C1). The copy number in Gloeobacter violaceus PCC 7421 is 2.
ISGvi2 was found by screening completely sequenced genomes for sequences homologous to the IS50R transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-96-D(N3)-138-E(C1). The copy number in Gloeobacter violaceus PCC 7421 is 2.
References
1] Nakamura,Y., Kaneko,T., Sato,S., Mimuro,M., Miyashita,H., Tsuchiya,T., Sasamoto,S., Watanabe,A., Kawashima,K., Kishida,Y., Kiyokawa,C., Kohara,M., Matsumoto,M., Matsuno,A., Nakazaki,N., Shimpo,S., Takeuchi,C., Yamada,M. and Tabata,S.(2003)DNA Res. 10(4), 181-201.
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18