ISSde1
- Family IS4
- Group IS10
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007954 | ND | Shewanella denitrificans | Shewanella denitrificans OS217 |
DNA section
IS Length : 1318 bp
Ends
IR Length : 18/21
IRL : CTAGGGAATCCCCTGATAATTGCTTTTATTTTCAATTTAGTAGACACGCA
IRR : CTGGCGGATCCCCTCATAATTTAGGTCTGCCAGCGGTATGAACCAGTTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGACCACAAA | GCTTTGAAG | TCCCCGCAAA | 9 |
| TGAACCTGAA | AGCCACACT | AAAGAGCAAT | 9 |
| TGTCAGTTAA | CACTTACAT | TAAACAACGA | 9 |
| ACGCCCCACT | GCTAAAAATA | 0 | |
| TATAACAGAC | CACTCATGGT | 0 | |
| ACGCTGCTAT | CGCCCCAATA | 0 |
DNA sequence
CTAGGGAATCCCCTGATAATTGCTTTTATTTTCAATTTAGTAGACACGCATGGTGCTATCTGATCTAATCAATTTCGCCAAAAACCTACTAGATCTAACA
CCATGCGTGATATCCATATCTTACACGATTTAATCAAAAAACAATGCCCTCAAATACACCAAAAACGACTTAATTCTCTGATGGTTGCCACTGAGTCACT
CCTCGATGGTAATCAACTATCACTTACTCAGCTTGGTCGTAATATTACGGGCTCCGTGGCAGCTAAACACAACATTAAACGTATTGACCGACTCTTAGGT
AATTATCACTTAGCTCAAGATAAAATAACAATATATCAGTGGCATGCTCGCTATCTTTGTGGCGCAAACCCTATGCCTATTATTCTCGTCGATTGGTCTG
ATGTCCGTGAACAGCTTCGAATAATGACGCTACGCGCATCCATCAGCGTTCAAGGTCGCTCTGTGACTCTGTATGAACGGACTTTCTTGTTTGAGGACTA
CAACGCACCTCGCAGCCATAATGCTTTTCTCGCTGAACTTGCCAACGTTTTACCTCAAGGTTGTTGTCCTCTGATTGTCACCGATGCAGGCTATCGCAAT
ACCTGGTTTAGGGAAGTTGAGCGCTATGGCTGGTTCTGGCTTGGCAGAGTGCGAGGCGATGTAAGCTTTATGCACCACGGGCAAACCACTTGGCATTCAA
ATAAATCACTCTACTCAAAAGCAAACTCAACCGCTAAATACATAGGTAATGTTCAGCTTGCACGTAAATCACCGCTAAGCTGCCATTTACATCTATTTAA
GGCTAAGTCCAAATTACGTAAAGATAAGCGCTCTTCAAAAACGGGCCGAAACCACACCGCACAAAAGAGTTATCGCTTAGGCAGTAAGGAGCCGTGGCTG
CTGGCGACCAACTTACCGCCTGAATACTTCAATCCAGCAAAAGTGGTTGAGCTATATGCTAAACGCATGCAAATTGAAGAAACCTTTCGGGATTTAAAAA
GCCCACAATATGGCATGGGATTGAGGCAAAGTCGCAGTCGGTGTCCAAAGCGATATGATGTATTGTTGCTTATCGCTATGCTGGCAGAAATACTGTTGTG
GTGTATAGGCATAGCCGCGAGGCATCTTGGCTGGCAGAAGGATTTCCAAGCTAACAGCATCAAATATCGCGCTGTATTATCCGTTGTAAGATTAGGAAAA
GAAGTGCGGCGAAGGCCAAAATACACACTAAAAGCATCGATAATTTACTGGGCATTAAACGAATATATCAAACTGGTTCATACCGCTGGCAGACCTAAAT
TATGAGGGGATCCGCCAG
CCATGCGTGATATCCATATCTTACACGATTTAATCAAAAAACAATGCCCTCAAATACACCAAAAACGACTTAATTCTCTGATGGTTGCCACTGAGTCACT
CCTCGATGGTAATCAACTATCACTTACTCAGCTTGGTCGTAATATTACGGGCTCCGTGGCAGCTAAACACAACATTAAACGTATTGACCGACTCTTAGGT
AATTATCACTTAGCTCAAGATAAAATAACAATATATCAGTGGCATGCTCGCTATCTTTGTGGCGCAAACCCTATGCCTATTATTCTCGTCGATTGGTCTG
ATGTCCGTGAACAGCTTCGAATAATGACGCTACGCGCATCCATCAGCGTTCAAGGTCGCTCTGTGACTCTGTATGAACGGACTTTCTTGTTTGAGGACTA
CAACGCACCTCGCAGCCATAATGCTTTTCTCGCTGAACTTGCCAACGTTTTACCTCAAGGTTGTTGTCCTCTGATTGTCACCGATGCAGGCTATCGCAAT
ACCTGGTTTAGGGAAGTTGAGCGCTATGGCTGGTTCTGGCTTGGCAGAGTGCGAGGCGATGTAAGCTTTATGCACCACGGGCAAACCACTTGGCATTCAA
ATAAATCACTCTACTCAAAAGCAAACTCAACCGCTAAATACATAGGTAATGTTCAGCTTGCACGTAAATCACCGCTAAGCTGCCATTTACATCTATTTAA
GGCTAAGTCCAAATTACGTAAAGATAAGCGCTCTTCAAAAACGGGCCGAAACCACACCGCACAAAAGAGTTATCGCTTAGGCAGTAAGGAGCCGTGGCTG
CTGGCGACCAACTTACCGCCTGAATACTTCAATCCAGCAAAAGTGGTTGAGCTATATGCTAAACGCATGCAAATTGAAGAAACCTTTCGGGATTTAAAAA
GCCCACAATATGGCATGGGATTGAGGCAAAGTCGCAGTCGGTGTCCAAAGCGATATGATGTATTGTTGCTTATCGCTATGCTGGCAGAAATACTGTTGTG
GTGTATAGGCATAGCCGCGAGGCATCTTGGCTGGCAGAAGGATTTCCAAGCTAACAGCATCAAATATCGCGCTGTATTATCCGTTGTAAGATTAGGAAAA
GAAGTGCGGCGAAGGCCAAAATACACACTAAAAGCATCGATAATTTACTGGGCATTAAACGAATATATCAAACTGGTTCATACCGCTGGCAGACCTAAAT
TATGAGGGGATCCGCCAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1200 bp | 400 aa | 103 | 1302 | + | No |
Chemistry : DDE
ORF sequence :
MRDIHILHDLIKKQCPQIHQKRLNSLMVATESLLDGNQLSLTQLGRNITGSVAAKHNIKRIDRLLGNYHLAQDKITIYQWHARYLCGANPMPIILVDWSD
VREQLRIMTLRASISVQGRSVTLYERTFLFEDYNAPRSHNAFLAELANVLPQGCCPLIVTDAGYRNTWFREVERYGWFWLGRVRGDVSFMHHGQTTWHSN
KSLYSKANSTAKYIGNVQLARKSPLSCHLHLFKAKSKLRKDKRSSKTGRNHTAQKSYRLGSKEPWLLATNLPPEYFNPAKVVELYAKRMQIEETFRDLKS
PQYGMGLRQSRSRCPKRYDVLLLIAMLAEILLWCIGIAARHLGWQKDFQANSIKYRAVLSVVRLGKEVRRRPKYTLKASIIYWALNEYIKLVHTAGRPKL
VREQLRIMTLRASISVQGRSVTLYERTFLFEDYNAPRSHNAFLAELANVLPQGCCPLIVTDAGYRNTWFREVERYGWFWLGRVRGDVSFMHHGQTTWHSN
KSLYSKANSTAKYIGNVQLARKSPLSCHLHLFKAKSKLRKDKRSSKTGRNHTAQKSYRLGSKEPWLLATNLPPEYFNPAKVVELYAKRMQIEETFRDLKS
PQYGMGLRQSRSRCPKRYDVLLLIAMLAEILLWCIGIAARHLGWQKDFQANSIKYRAVLSVVRLGKEVRRRPKYTLKASIIYWALNEYIKLVHTAGRPKL
Blast result :
Comments
ISSde1 is 72% aa similar to IS10A.
ISSde1 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-63-D(N3)-130-E(C1).The copy number in Shewanella denitrificans OS217 is 6.
ISSde1 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-63-D(N3)-130-E(C1).The copy number in Shewanella denitrificans OS217 is 6.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Kiss,H., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A. and Richardson,P.(2006)Unpublished.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Kiss,H., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A. and Richardson,P.(2006)Unpublished.