ISNpu4
- Family IS4
- Group IS10
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Nostoc punctiforme | Nostoc punctiforme PCC 73102 |
DNA section
IS Length : 1272 bp
Ends
IR Length : 14
IRL : CTGAAGGGGGGACAAGCACCCGAAAGCCTTTGTGCTGTATTAAGTTGAGC
IRR : CTGAAGGGGCGACATGAAGCCTATAAAGCTGACTGTATAAGCTTCATAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAATCAGCTA | NAACTAACC | TATACTGGCT | 9 |
DNA sequence
CTGAAGGGGGGACAAGCACCCGAAAGCCTTTGTGCTGTATTAAGTTGAGCGAATTATGGTAAAAAAAGATAGGTCAGTATTGTCAATAAGACCTATCTGT
TGAAAATGTTACCTGAATTATACCAGAAGCATCTACAAAGTTTACTTTCACAGTCCGAATTAATATTTCTGACGCTGGTGATTAACGTAGTACAAAATAT
CAAAGATGTGAAACTAGAGAAAATATCAGAGTCGCTACCATTATTCATTCAATGCCAGTCGAGACGGAAGAAGTTACAAAGGTTTCTATTATTACCAATA
TTGAATATAGAAGAATTATGGTTTCCCATAATTGAACGATGGTTAGCTCAAATTTTCCTGGGAAATCACCGAATCTATTTAGCAATTGATAGGACGAATT
GGAAAAGAAAAAATCTACTAATGATTAGTGTAATTTTTCAAAAAAGAGCTATACCGATATACTTTAAGCTTTTAGCAAAATTAGGTAGTAGTAACTTATC
AGAACAAACTAAGGCATTAACAAAGATAATTCCCTTATTTAAAAACTATAAAACGGTAGTGTTAGGAGATAGAGAGTTTTGTTCAGTGAGCTTGGCGAAA
TGGCTTGATGAACAAGGGTTTGAGTTTTGTTTGAGACTCAAGAAAAATGAGAATATTGAGTTGAAAGCTCATTTATGGTGCGAAATAAAAGATTTAGGTT
TAAAACCAGGAACATCTTTTTTTGTATCAGATGCAACGGTAACAAAAACTAAACAAGTGAAGGGATTTAATGTGGCTTGCAAATGGAAAAAGAATTACCG
TCAAAACAAAGCCAAAGAAGGATGGTTTATTTTAACTAACATGAATAGTAAAATAACGGCGATTCAGGCATATCAAAAGCGCTTTGATATAGAAGAGATG
TTTAGAGATTTTAAAAGTGGCGGTTACAATTTAGAGAAGACAAATGTGGAAGGTAAGCGGTTTATTGCTTTAGTTTTAATCATCTCATTAGCTGACACTA
TTGCTACATTACAAGGTCAGAATATTAAGAGTAAAGGAATTGCAAAATATCTGGCACGTCCCAAAGAATATGGACGTTCTCACAGAAGGCATAGTAACTT
TTATATCGGTCTTTATGCACAGAACTGGGTTAATTTCATTGGTGATTGTTGGAGTTTAGTTCAAGATTTAATGCGATTAAGTCGTCATAAACTAGAGAAT
TATTTATTTACAAGGGATGAGAGCTATGAAGCTTATACAGTCAGCTTTATAGGCTTCATGTCGCCCCTTCAG
TGAAAATGTTACCTGAATTATACCAGAAGCATCTACAAAGTTTACTTTCACAGTCCGAATTAATATTTCTGACGCTGGTGATTAACGTAGTACAAAATAT
CAAAGATGTGAAACTAGAGAAAATATCAGAGTCGCTACCATTATTCATTCAATGCCAGTCGAGACGGAAGAAGTTACAAAGGTTTCTATTATTACCAATA
TTGAATATAGAAGAATTATGGTTTCCCATAATTGAACGATGGTTAGCTCAAATTTTCCTGGGAAATCACCGAATCTATTTAGCAATTGATAGGACGAATT
GGAAAAGAAAAAATCTACTAATGATTAGTGTAATTTTTCAAAAAAGAGCTATACCGATATACTTTAAGCTTTTAGCAAAATTAGGTAGTAGTAACTTATC
AGAACAAACTAAGGCATTAACAAAGATAATTCCCTTATTTAAAAACTATAAAACGGTAGTGTTAGGAGATAGAGAGTTTTGTTCAGTGAGCTTGGCGAAA
TGGCTTGATGAACAAGGGTTTGAGTTTTGTTTGAGACTCAAGAAAAATGAGAATATTGAGTTGAAAGCTCATTTATGGTGCGAAATAAAAGATTTAGGTT
TAAAACCAGGAACATCTTTTTTTGTATCAGATGCAACGGTAACAAAAACTAAACAAGTGAAGGGATTTAATGTGGCTTGCAAATGGAAAAAGAATTACCG
TCAAAACAAAGCCAAAGAAGGATGGTTTATTTTAACTAACATGAATAGTAAAATAACGGCGATTCAGGCATATCAAAAGCGCTTTGATATAGAAGAGATG
TTTAGAGATTTTAAAAGTGGCGGTTACAATTTAGAGAAGACAAATGTGGAAGGTAAGCGGTTTATTGCTTTAGTTTTAATCATCTCATTAGCTGACACTA
TTGCTACATTACAAGGTCAGAATATTAAGAGTAAAGGAATTGCAAAATATCTGGCACGTCCCAAAGAATATGGACGTTCTCACAGAAGGCATAGTAACTT
TTATATCGGTCTTTATGCACAGAACTGGGTTAATTTCATTGGTGATTGTTGGAGTTTAGTTCAAGATTTAATGCGATTAAGTCGTCATAAACTAGAGAAT
TATTTATTTACAAGGGATGAGAGCTATGAAGCTTATACAGTCAGCTTTATAGGCTTCATGTCGCCCCTTCAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1167 bp | 389 aa | 106 | 1272 | + | No |
Chemistry : DDE
ORF sequence :
MLPELYQKHLQSLLSQSELIFLTLVINVVQNIKDVKLEKISESLPLFIQCQSRRKKLQRFLLLPILNIEELWFPIIERWLAQIFLGNHRIYLAIDRTNWK
RKNLLMISVIFQKRAIPIYFKLLAKLGSSNLSEQTKALTKIIPLFKNYKTVVLGDREFCSVSLAKWLDEQGFEFCLRLKKNENIELKAHLWCEIKDLGLK
PGTSFFVSDATVTKTKQVKGFNVACKWKKNYRQNKAKEGWFILTNMNSKITAIQAYQKRFDIEEMFRDFKSGGYNLEKTNVEGKRFIALVLIISLADTIA
TLQGQNIKSKGIAKYLARPKEYGRSHRRHSNFYIGLYAQNWVNFIGDCWSLVQDLMRLSRHKLENYLFTRDESYEAYTVSFIGFMSPLQ
RKNLLMISVIFQKRAIPIYFKLLAKLGSSNLSEQTKALTKIIPLFKNYKTVVLGDREFCSVSLAKWLDEQGFEFCLRLKKNENIELKAHLWCEIKDLGLK
PGTSFFVSDATVTKTKQVKGFNVACKWKKNYRQNKAKEGWFILTNMNSKITAIQAYQKRFDIEEMFRDFKSGGYNLEKTNVEGKRFIALVLIISLADTIA
TLQGQNIKSKGIAKYLARPKEYGRSHRRHSNFYIGLYAQNWVNFIGDCWSLVQDLMRLSRHKLENYLFTRDESYEAYTVSFIGFMSPLQ
Blast result :
Comments
ISNpu4 is 58% aa similar to IS8402.
ISNpu4 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-59-D(N3)-107-E(C1).
Genome in progress : Accession number : NZ_AAAY02000041.1
ISNpu4 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-59-D(N3)-107-E(C1).
Genome in progress : Accession number : NZ_AAAY02000041.1
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] NCBI Microbial Genomes Annotation Project(2006)Unpublished.
2] NCBI Microbial Genomes Annotation Project(2006)Unpublished.