ISfinder Mirror Site

ISMmg3

Family IS4
Group IS10

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Magnetospirillum magnetotacticum	Magnetospirillum magnetotacticum MS-1

DNA section

IS Length : 1189 bp

Ends

IR Length : 17/18

IRL : CTCACCACCCATCAAATTTCCTTGACCTGAAGAATCGACGTGTGATTCGA
IRR : CTCACCACCCATCAGATTCTCCAAACGGCCCAGAGTGGTGGCAGGCCCCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGGCGTCTAT	CGGTGACCAC	GACTTGGGGA	10

DNA sequence

CTCACCACCCATCAAATTTCCTTGACCTGAAGAATCGACGTGTGATTCGAAGAGTCCGCCGGATTTCATGGGGGCGGAGGAGCGATGAAGGGTGGGATCG
AAGTTATCGCGGAGACGATCTGCGAGGACATGCGTGTGCGGCTTCCCCGGCAGAACAAGAAGCAACGGGAAGGTCTGGCGCTTCTGGCTGCGACGATGCT
TGATGTTCGCAGCGCCAACCTGATGGACGTGGCGGCGTCGTTGCCTCGACAGGCCGAGCGGCTGGACATGCGTTACCAATGGATCAGCCGCGTGTTGGGC
AACGCGCTGATCGATGTCGACGAGGTGATGGCTCCTTACGTTCGGGACATTCTGGGGCGTCTGGTGGGAGACGGGCGGCGGTTGGTGTTGATCATCGACC
AGACCCAGGCGAACGAGGTGCAGCAGGCGGTCGTGGTGGCCGTCCGTGTCGGCGAACGATCTTTGCCCCTGGCGTGGCGGGTCAAGAAGACGCAAGGGGC
GATTGGTTTTGCCGAACAGCGCGAAGCCCTTGAGGTCGTGGCGGGGCTGTTGCCCGAGGGCGTGCGTCCGGTGCTGATGGGCGACCGGTTCTACGGCAGC
CCCGACCTGATTGCCTGGTGCCGGACGCAGAGCTGGGATTGGCGCTTGCGGCTGAAGCAGGATCTTCTGGTCTTCGAAGACGGCGGCGAAAGCACCTTGG
CCGAGTGCTTCGCGCGCGGCGAGCGCATGCTGACCGGCGTCGAGTTGACCGGGAAACGGGTGCCGACCAACGTCGCCATGGTTCATGAAGCGGGCCATCC
GGAGCCGTGGATTATTGCCTTGTCCGAAGCCCCGACCGTCCATCGCGCCTTCGATTACGGCCTGCGCTGGGGTATCGAGGCCATGTTCTCCGACTTCAAG
ACCAGGGGTTTCGGGCTGGAAGACAGCCATATTCAGCGCGCTGACCGGATGGATCGTCTCATCATGGTGATGGCGCTCGCCCTGTTCTGGGCGGTCTCCA
CTGGGATGTGGGCGGCCGTCCATGCGGCATCACCCGACGAAAAAAAGACAGGCCAAACCAGCCCAAGAACGTCATCCGAAGCATGATGTCGTTCTTCAAG
CGCGGCATCCGCCGTATCCAGACTTGCCTTCAGCGCCTATGGGGCCTGCCACCACTCTGGGCCGTTTGGAGAATCTGATGGGTGGTGAG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
999 bp	333 aa	85	1083	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKGGIEVIAETICEDMRVRLPRQNKKQREGLALLAATMLDVRSANLMDVAASLPRQAERLDMRYQWISRVLGNALIDVDEVMAPYVRDILGRLVGDGRRL
VLIIDQTQANEVQQAVVVAVRVGERSLPLAWRVKKTQGAIGFAEQREALEVVAGLLPEGVRPVLMGDRFYGSPDLIAWCRTQSWDWRLRLKQDLLVFEDG
GESTLAECFARGERMLTGVELTGKRVPTNVAMVHEAGHPEPWIIALSEAPTVHRAFDYGLRWGIEAMFSDFKTRGFGLEDSHIQRADRMDRLIMVMALAL
FWAVSTGMWAAVHAASPDEKKTGQTSPRTSSEA

Blast result :

Comments

ISMmg3 is 42% aa similar to IS1481.
ISMmg3 was found by screening completely sequenced genomes for sequences homologous to the IS10R transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-61-D(N3)-97-E(C1).
Genome in progress : Accession number : NZ_AAAP01003868.1

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] NCBI Microbial Genomes Annotation Project(2006)Unpublished