ISPto3

  • Family IS4
  • Group ISPepr1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Psychroflexus torquis
Psychroflexus torquis ATCC 700755
DNA section
IS Length : 1572 bp

Ends


IR Length : 17/19

IRL : CTCAAGTTTCGCACCCATTATTCACAGGGTTTAATTGGAAAAAAATAGCA
IRR : CTCAAGTTTCGCACCTAATCAATTTCACTTAACACCTTGATGATTAAAGC

Insertion site


Left flankDirect repeatRight flankDR Length
CTTTTTTTTATTAAAAATAATTTCAAT7

DNA sequence

CTCAAGTTTCGCACCCATTATTCACAGGGTTTAATTGGAAAAAAATAGCATGAAAACCAAGGGAAACCCCTGGTTTTACTGTATCTTTAAGTAACTAAAC
ACAAAGTACAAAACATGCTACAACACGAATATATTGCAAAAGTTCAAGAAATAAAAGGGAAGTTTAATAAGGTTTGGTTTAATTCAGACTATTTACGGGC
ACATCTGAATATTTTAGGCTTCAATAGAATAAAAAAACAATTCAGTTGGTGCAAAAAGGCGGGTTTTTCATTTGAGGATTTGATCGCTACGCTCTTGATT
TTGCCCCTTATTGGAATTAATTCTATTTATGGACTTACAACTGACAAAGATCCAGAACTTAATAAATGTGGAAAAGATTCATACTATCGAATCTTGGCAA
ATCAAAAAATTAATTGGAGAGCTTTTTTGGCCCAGTTTGTAAAGCAATATCTATTGAAAGATGAACTCTTCACCCCCTCAGCAGACCCTACAAGATGCTT
GATCTTTGATGATACTGATCTTTCAAAAACAGGAAAGACTATTGAAGGAGTCTCAAAGATCTACAACCATGTGTCAAAGACCTACTATTTAGGTTTCAAG
CTACTTGTGGCTGGGTATTGGAATGGAAGTGTTTTTATCCCCATTGATTTTAGTCTGCACCGTGAGAGCAAAACATCAAGATTAAAATATGGTCTAACCG
CAAAGCAGCGTAAGGCCCAAAAGAAGACACCAAGATGTAGTAAAACAGTTGCGGCAAAGAGGTATAGGGAACTCAATAAAAAGAAAACAGACCTAGTAGT
GCAAATGTTTTCCAGGGTTGTAAAGCGTAAAATTCCTGTAGACTATATTTTAATAGATACCTGGTTTACAAGTGTGGGATTACTTAAAAAGTTACGAAGT
ATTTGCAGTTCGACCCATATTATTGGAATGTATAAATACAATAGTAAAATTGAAGTCAGATCAAAGGTCAAAACTTTAGCACAACTTAAAAAACAGAAAG
CAAAGCCCAAGCGCTGTCGTAAATTCAATTACTACTACCATCATTACATAGCTGAAATTGATGGGCTCAAGGTTGCTCTCTTTATCTCAAAACGTGGGAA
GAACGGCAAATGGCACACCTTAATAACCACCGATACATCACTCAAATTTGTAAAAGCAATTGAAGTATATAGTATTCGATGGTCAATTGAAGTCTTTTTT
AAAGAAGCCAAGCAGCTCTTTGGCCTTGGAAAGTGTCAGTCTACCAATTTTGATGTCCAGATTGCACAAATAACAATTGCAATGACCCAATATCTGCTTA
CAAGTATTAGGTACAGAATGGAGGCTTATGAAACCATAGGCGGATTATTTAAAGATTTAAAACAGGATTATATTGAAAATAAGCTGAATATCAGAATATT
GGCAGTTGTCAACTTAATTTTAACCAATTTGGAAAAACTAGTAGAATCAATTGATATTGAACTTATTACATCCAAAATAATAGAGGATATTGAGAGTTAT
GGGTTTCTAACAAATACCCATAGCTTTAATCATCAAGGTGTTAAGTGAAATTGATTAGGTGCGAAACTTGAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1431 bp477 aa1151545+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLQHEYIAKVQEIKGKFNKVWFNSDYLRAHLNILGFNRIKKQFSWCKKAGFSFEDLIATLLILPLIGINSIYGLTTDKDPELNKCGKDSYYRILANQKIN
WRAFLAQFVKQYLLKDELFTPSADPTRCLIFDDTDLSKTGKTIEGVSKIYNHVSKTYYLGFKLLVAGYWNGSVFIPIDFSLHRESKTSRLKYGLTAKQRK
AQKKTPRCSKTVAAKRYRELNKKKTDLVVQMFSRVVKRKIPVDYILIDTWFTSVGLLKKLRSICSSTHIIGMYKYNSKIEVRSKVKTLAQLKKQKAKPKR
CRKFNYYYHHYIAEIDGLKVALFISKRGKNGKWHTLITTDTSLKFVKAIEVYSIRWSIEVFFKEAKQLFGLGKCQSTNFDVQIAQITIAMTQYLLTSIRY
RMEAYETIGGLFKDLKQDYIENKLNIRILAVVNLILTNLEKLVESIDIELITSKIIEDIESYGFLTNTHSFNHQGVK

 

Blast result :
Comments
ISPto3 is 53% aa similar to ISBf8. ISPto3 was found by screening completely sequenced genomes for sequences homologous to the ISRm16 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-114-D(N3)-110-E(C1). The sequencing of the Psychroflexus torquis ATCC 700755 chromosome is currently in progress. Accession: NZ_AAPR00000000.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Bowman,J., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Unpublished