ISfinder Mirror Site

ISLre1

Family IS4
Group ISPepr1

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Lactobacillus reuteri	Lactobacillus reuteri 100-23

DNA section

IS Length : 1543 bp

Ends

IR Length : 14

IRL : CTGAAACTTGAAAGCGCAGAATTGCAGTAATTAACCGCCATTCTGCGCTT
IRR : CTGAAACTTGAAAGTACTCAAAAGGACCGGAGCCGCTATCAGCTCTGGCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAAAAATAGA	TTAG/TT/GAGA/T	AAAAAGAATA	14

DNA sequence

CTGAAACTTGAAAGCGCAGAATTGCAGTAATTAACCGCCATTCTGCGCTTTTTCCTTATAAAAATAGAAAACGCAAGAACACTCGCGTTATACTTTAAGT
GACCAAACCAAAGTAAAGGAAGTTCTTACGCAATGAAAAGTATAAACCAAATTCAAGCAGAAAACGATACTAAAAAACTAATTTCTTCATTTATTCGGCT
AATTGGTCTAGGTCAGATTAGTAACCGAATTAATTTTAAACGACATTCAACTATTTCTTTAACCGCCATCATTACTTGGCTGTTTGAAGTCACCTTTAGT
TGCCGTTCTCTTTATCGAGCACAACCCAGTCAGCTATTTAGCCCGCGTACCGCTCGAAATGTGCTAAATGATGGAAGAATCAATTGGCAAAAACTACTAT
GTCTTGTGGCAGTTAAAATAATTTCATTACTTCGTCCATTAATTGATCGACGGCGACGACTGGCCTTTATCGTTGACGATACGTTAATTTCTCGTCCATA
CTCAACTAAAACTGAACTCTTGGCAAAAGTATATGATCATAATCAGGACAAATATATAACTGGTTACCGTAATTTAACAATTGGCTGGAGCGATGGTAAC
ACTTTTTTGCCAGTTAATTTTGCTTTAATGTCAACTAAAAATAGAGCCAACTTAGTTGGCACCAAGGCTTGTGTAACTGATCAACGAACGATTGCTGGTC
AACGACGCAACCAAGCGCAAAGAAAAATGAATGATGTGGTAATTGAATTGATTCATCAAGCTCTTAAGTTTGGTATATCAGCTAAATACGTTCTTTTTGA
TAGCTGGTATAGTTCTCCACGAATGTTTTGGCGGCTTAAAGAACTAGGATTAGATAGTGTAGCTATGCTTAAGCGAAGCTCTAAAGTTTATTACCGATAT
CGTGGACGTGCCTATAGTATCAAAGCATTATATTTACGACTACTTCATTCAAAACGCCATCAAGCTGAGAACTATCTTTATAGCAGTAACGTTGAAGCTA
ACTTTCAGGGATATAGTTTTCCGCTAAAAGTTGTTTTCGTAGCTAAAAAAGGTACCAAGAATCAATATTTGGTTCTAGCCTCAACTAACACTAAGCTAAC
ACCACAGAAAATTATTCAATTATATAATCGCCGATGGTCAATTGAGACTTACTTCAAAACAGCTAAACAATACTTACGACTTAATAAATCGCAAATTCAA
AGTTATGATGGTCAGGTTGCACAAATTACGATTACTGCGCTGACTTTTATCCTATTAGCTTGGCAAGAACGTCAAAGTAAGGACGATCGTACTTTAGGTG
ATTTGTTTTACCTGATGAATGATGCCCTTCCAGAAATTAAATTTATTGAAGCATTGGTCTACCTATTAAAAACACTAGAATCACACGAAACTGTTTTTCT
TAATCAAACAATTAGTCAATTTATGAATTACTTACCTGAAAATATTCAAAACGCTCTTCATGAAGCTGTTTGAAACTAATTTTAGTAAAGAAAAGCCAGA
GCTGATAGCGGCTCCGGTCCTTTTGAGTACTTTCAAGTTTCAG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1338 bp	446 aa	133	1470	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKSINQIQAENDTKKLISSFIRLIGLGQISNRINFKRHSTISLTAIITWLFEVTFSCRSLYRAQPSQLFSPRTARNVLNDGRINWQKLLCLVAVKIISLL
RPLIDRRRRLAFIVDDTLISRPYSTKTELLAKVYDHNQDKYITGYRNLTIGWSDGNTFLPVNFALMSTKNRANLVGTKACVTDQRTIAGQRRNQAQRKMN
DVVIELIHQALKFGISAKYVLFDSWYSSPRMFWRLKELGLDSVAMLKRSSKVYYRYRGRAYSIKALYLRLLHSKRHQAENYLYSSNVEANFQGYSFPLKV
VFVAKKGTKNQYLVLASTNTKLTPQKIIQLYNRRWSIETYFKTAKQYLRLNKSQIQSYDGQVAQITITALTFILLAWQERQSKDDRTLGDLFYLMNDALP
EIKFIEALVYLLKTLESHETVFLNQTISQFMNYLPENIQNALHEAV

Blast result :

Comments

ISLre1 is 54% aa similar to ISGur1. ISLre1 was found by screening completely sequenced genomes for sequences homologous to the ISGur1 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-106-D(N3)-114-E(C1). The sequencing of the Lactobacillus reuteri 100-23 chromosome is currently in progress. Accession: NZ_AAPZ00000000.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Bruce,D., Pitluck,S. and Richardson,P. (2006) Unpublished