ISCku4

  • Family IS4
  • Group ISPepr1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CT573073 ND Candidatus Kuenenia
Candidatus Kuenenia stuttgartiensis
DNA section
IS Length : 1971 bp

Ends


IR Length : 16/19

IRL : CTTGAGAGTACCGAGTTCTCCATGTTGTGAATCTATTTAATCATATCTAC
IRR : CTTGAGAGTACCGTATTTTACAGTAGGCACTAATCCCATTCTCACGAGTA

Insertion site


Left flankDirect repeatRight flankDR Length
AACATTTGTTTATTGGAGAAAAGAGGC7
TTAAAAATAGTATTAAAGAAATTAAA6
ACCAATATTATTTCTGAAGAAATTAAA7
ATTGAATGTCTGTTTTACATATTCAAC7

DNA sequence

CTTGAGAGTACCGAGTTCTCCATGTTGTGAATCTATTTAATCATATCTACAATTCTTATTTTTTACAGTACAATTTTAAACTTATTTCGGTTTGTCACGT
TTCGCTAAATGGGCCTTCAATCGTGAATGTTCAATAGAAAGATCTATCCATAATTCAATCAATTTTTTAAACTCTTTATATGTTTTTAACAGTTGCTTTG
TCTCATCGACAAACGAAGGCCTGACGTATTCCGTCCTGCTTTTCATTTTATGGGTAAAACTGAGTTGATAATATGCCCCCTTTTTCCCCGCAGGGTCCTT
ATATTGTTTACTCAGACTACCTGGTCTCATTTCATTAATTGACTGAAGTTTCTTTTTGATTTTTTTAATCTTCTCTTCAATTTGTTGTAGCTTTTTTATT
GACATATATGTAGCATATACAGGCTACATCAATACGTCAACAATAATTTTAAGAGGAGGAAGATTTATGAAGCTTTGGATTCTGTGGTGGAGCACGGTCT
GGCAACTACGCCCAGCCTGCTCTCGCCTGAGAAACTTTTTATGGTTTGCTACAGTTTTAGCCGGAATTACCGTTCGGCCTGATTTGCTCGGGGTGTCTAG
TATTATACGTGGACTCGGCCTTAAGGGGTATTGTTATGACCGCCTTTTGGATTTTTTTCATAGCAATGCACTAAATGTTGAGAAACTAACCAAACTATGG
ACAAAGCTTATTATAAAAACACATCCGCAGGTAAAGCGTTTTCGCGGGAAATTGCTTTTATGCGGGGATGGCCTTAAGGTGCCAAAAGAAGGCAAAAAGA
TGCCTGGTGTAAAGTCATTGCATCAGGAATCTGACTCGAATAACAAGGCAGAGTACATTATGGGCCATTCCTGTCAGGTTGTTTCTCTTTTAGCTGAAGC
CGGGAAAAGTTGTTTTGCTATTCCACTGGTGAGCAGAATTCATGAAGGCGTTGTCTTTTCAAACAGAGACCAAAGAACCTTACTCGACAAAATGGTTTTG
TTGATTAATTCTCTGGAGCTGAAAGAATTGTTCTACTTTATTGCAGACGCCTATTACGCATCCCATGCTATTATTAATGGCGTAGTTGCCCGGGGCAGCC
ACTTGATTAGCAGGGTAAGGAGTAATGCCGTTGCATACTTTCCCGTGGAACCGACTCCTGAAAAGAAAGGACGTGGACGACCCAAAAAATATGGTATGAA
AGTAAAATTAAAAACATTGTTGAATGATCGCGCATCAATGAAGGAGGCGGAAAGTCCGGTTTATGGAGAGCAAGGCATAAAAATAAATTATAGAACCCTT
GATCTGCTTTGGAAACCTGTGGGTATTCTTATTAGATTTGTATTGGTGGATCACCCTCAAAGAGGGAAAATTATTTTAATGAGTACGGATTTAACTATTA
GTGCAATGGAAATTATCTGCCTGTATGGATTACGTTTCAAAATCGAAGTGTCTTTCAAACAGGCGCTTAGAACCTTGGGCACATATGCCTACCATTTCTG
GATGAGAAACATGCAGCCAATTAAAAGGCGTTCCGGCAATCAACATGTACACAAAAGAAGCGCTGAATACAGAAATGCAGTTCGCAGAAAACTTGCAGCC
TATCACAGACATATACAGGCCGGCGTTATTGCACAGGGGCTTTTGCAATATATTTCTTCAGCATTTCCATCGCTTGTTTGGAATTCCTTCGGGTCTTGGT
TGCGGACTATGAGGCCGGGAATTTGTCCTTCAGAACAAGTAACGGCCATTGCAATGAGAAATTGCCTTCCTGAATTTCTCGTGGATTCATCTCAAAAGTC
AATCCTTACAAAATTTATTCTTGAACGAATCGATTTTTCCAGGGCAGAAGGGGCTCGATTGGTCGCATAGTTGCTTTTACTACACTTTTTAACTATTTTA
TTTTCACTCACAAGGTATTTTTACTCGTGAGAATGGGATTAGTGCCTACTGTAAAATACGGTACTCTCAAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1401 bp467 aa4671867+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKLWILWWSTVWQLRPACSRLRNFLWFATVLAGITVRPDLLGVSSIIRGLGLKGYCYDRLLDFFHSNALNVEKLTKLWTKLIIKTHPQVKRFRGKLLLCG
DGLKVPKEGKKMPGVKSLHQESDSNNKAEYIMGHSCQVVSLLAEAGKSCFAIPLVSRIHEGVVFSNRDQRTLLDKMVLLINSLELKELFYFIADAYYASH
AIINGVVARGSHLISRVRSNAVAYFPVEPTPEKKGRGRPKKYGMKVKLKTLLNDRASMKEAESPVYGEQGIKINYRTLDLLWKPVGILIRFVLVDHPQRG
KIILMSTDLTISAMEIICLYGLRFKIEVSFKQALRTLGTYAYHFWMRNMQPIKRRSGNQHVHKRSAEYRNAVRRKLAAYHRHIQAGVIAQGLLQYISSAF
PSLVWNSFGSWLRTMRPGICPSEQVTAIAMRNCLPEFLVDSSQKSILTKFILERIDFSRAEGARLVA

 

Blast result :
Comments
ISCku4 is 46% aa similar to ISDra7. ISCku4 was found by screening completely sequenced genomes for sequences homologous to the ISGur1 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-92-D(N3)-132-E(C1). The copy number on the Candidatus Kuenenia stuttgartiensis chromosome is 4. One copy lies on fragment 5 (Accession: CT573071).
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Strous,M., Pelletier,E., Mangenot,S., Rattei,T., Lehner,A., Taylor,M.W., Horn,M., Daims,H., Bartol-Mavel,D., Wincker,P., Barbe,V., Fonknechten,N., Vallenet,D., Segurens,B., Schenowitz-Truong,C., Medigue,C., Collingro,A., Snel,B., Dutilh,B.E., Op den Camp,H.J., van der Drift,C., Cirpus,I., van de Pas-Schoonen,K.T., Harhangi,H.R., van Niftrik,L., Schmid,M., Keltjens,J., van de Vossenberg,J., Kartal,B., Meier,H., Frishman,D., Huynen,M.A., Mewes,H.W., Weissenbach,J., Jetten,M.S., Wagner,M. and Le Paslier,D.(2006)Nature 440 (7085), 790-794