ISSav1
- Family IS4
- Group IS4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003155 | ND | Streptomyces avermitilis | Streptomyces avermitilis MA-4680 |
DNA section
IS Length : 1776 bp
Ends
IR Length : 36/37
IRL : CAAGACCACGGAGTTAAGGCCGATCCGGGGTTGTCAACGGGATCCGGGAT
IRR : CAAGACCACGGAGTTAAGGCCGATCCGCGGTTGTCAAATGGGGCGGGACC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAGCTCATCA | GNGTACNTC | CGGGGAGCAG | 9 |
| CAACTCCAGC | GAGTACTTT | CGGGGAGCAG | 9 |
DNA sequence
CAAGACCACGGAGTTAAGGCCGATCCGGGGTTGTCAACGGGATCCGGGATTTTCGGGGAAGCGCAGGTCATGGCAACGGGGCTCCTGGTAGAGCGAGGAA
GCCGACCAAGGTCTTCCCACTCGAAGGAGCCCCGTTGCCCGTTCAGTGTTCCACCGTCACGCTCACGTCGTCCATCACCGTCGCCGACGGCATCTTCGCT
CCGGGGCATCTTGGCGAGCTGACCCAGCAGTTACCCTTCGAACTCGTCGATGACGTGCTGGAACGCGCCGGCGGCGCCCAGCACAGGCTGCGGCTGCTGC
CGTCGAGGGTGGGCGTGTACTTCGTGCTGGCACTGGCCCTCTTCCCGCAGCTGGGGTACGTGCGCGTGTGGGACAAGCTCACCGCCGGGCTGCGCGGGAT
TCTTCACCGGCGCCCTTCGGAGAAGGCGTTGCGGGAGGTTCGCCGGAGGCTGGGCGTCGCGCCGCTTCGGTTGCTGTTCGAAACGCTGGCCGGCCCTGTG
GCACAGCCGATCACACCCGGGGTGCGATACCGGTGCTGGCGCACGGTCGCCTTCGACGGGTGCAGCTCCACCAAGGCCCCTGACCGGCCGCGAGTCTGTG
CGTGGCTGGGCAAGCACAAGCACCGCTACGGCACCGACGGATACCCAATGCTGAAGATCATGGTGCTGTGCGAGACCGGGACTCGGGCCCTGCTCGGCGC
GGTCTTCGGACCGACGCCAGAGAAGGAGACCGGCTACGCCGAGCAACTGCTACCGCTGCTGGACGGCGGCATGCTGCTGCTCAACGACCGGGGCTTCGAC
TCCGACGACTTCCTCGCGAAGGCTGCGGCCACCGGCGCACAGCTGCTGGTGCGGCTCAAGGGAACCCGGACCCCCGCCCGCTGGGCGCTACTGCCGGACG
GGTCGTTCCTGACCAGGATCAACGGGACCCGGCTGAGGGTCATCGACGCGCACATCGCGGTGACGACCGCCAAAGGGCTGCGGCTGGAAGGCCACTACCG
GCTGGCCACCACGCTGACCGATCACCGCCGCTACCCCGCCGTCGAGCTGGTGGAGCTCTACCACGAGCGATGGGAGATCGAATCGGCGTTCTACTCGCTC
CGCCACACTTTGCAGTGCGGCCTGGTGCTGCGCTCCCAGGACGTGGCCGGGATCCAGCAGGAGCTATGGGCTCATCTGACCGTCTACCAGGCACTGCGCC
GGGCGATGGTCGAGGCCGTCGAGACGCTTCCCGGCACGGATCCGGACAGGGCTTCCTTCACCGTCGCCCTGGAGACCGCCAAGGAACAGCTCATCACCGC
GGCCAATGTGCTGCCAGACGCCGGACCAGGGCGCATCACATCGGCCCTGCTGCACGATCTACTCCCACCCCGCCAGGCCCGCGTCAACCCGCGCCGCGTC
AAGTGCCCGATCTCCCGCTACGCCGCCCCGCCCGACCAGGCCCAAGCCCTCGGGGCCTCCAGGATCACCAGCATCGCCGTCACCGTGCACTCGTCCGCCG
GCACCGGCTCTGACGGGCGTCGCGACCACACGCTGCAACTCCTGCGCACCAACCCTCTCCGCACCTGGCGCGCATGCGAAATCGCCCGCGGTATCGGACT
GGACGACGCTCGAGGACTACGCGCGGAACTGGGGCGCTGGGTCCGCGAAGGAATCCTTCGCCGCACCGGGCGAGGCATCTACACCCTCGAACCCGAGTGG
ATCACACCCGACCTCCACCACCCCTCGGTCCCGCCCCATTTGACAACCGCGGATCGGCCTTAACTCCGTGGTCTTG
GCCGACCAAGGTCTTCCCACTCGAAGGAGCCCCGTTGCCCGTTCAGTGTTCCACCGTCACGCTCACGTCGTCCATCACCGTCGCCGACGGCATCTTCGCT
CCGGGGCATCTTGGCGAGCTGACCCAGCAGTTACCCTTCGAACTCGTCGATGACGTGCTGGAACGCGCCGGCGGCGCCCAGCACAGGCTGCGGCTGCTGC
CGTCGAGGGTGGGCGTGTACTTCGTGCTGGCACTGGCCCTCTTCCCGCAGCTGGGGTACGTGCGCGTGTGGGACAAGCTCACCGCCGGGCTGCGCGGGAT
TCTTCACCGGCGCCCTTCGGAGAAGGCGTTGCGGGAGGTTCGCCGGAGGCTGGGCGTCGCGCCGCTTCGGTTGCTGTTCGAAACGCTGGCCGGCCCTGTG
GCACAGCCGATCACACCCGGGGTGCGATACCGGTGCTGGCGCACGGTCGCCTTCGACGGGTGCAGCTCCACCAAGGCCCCTGACCGGCCGCGAGTCTGTG
CGTGGCTGGGCAAGCACAAGCACCGCTACGGCACCGACGGATACCCAATGCTGAAGATCATGGTGCTGTGCGAGACCGGGACTCGGGCCCTGCTCGGCGC
GGTCTTCGGACCGACGCCAGAGAAGGAGACCGGCTACGCCGAGCAACTGCTACCGCTGCTGGACGGCGGCATGCTGCTGCTCAACGACCGGGGCTTCGAC
TCCGACGACTTCCTCGCGAAGGCTGCGGCCACCGGCGCACAGCTGCTGGTGCGGCTCAAGGGAACCCGGACCCCCGCCCGCTGGGCGCTACTGCCGGACG
GGTCGTTCCTGACCAGGATCAACGGGACCCGGCTGAGGGTCATCGACGCGCACATCGCGGTGACGACCGCCAAAGGGCTGCGGCTGGAAGGCCACTACCG
GCTGGCCACCACGCTGACCGATCACCGCCGCTACCCCGCCGTCGAGCTGGTGGAGCTCTACCACGAGCGATGGGAGATCGAATCGGCGTTCTACTCGCTC
CGCCACACTTTGCAGTGCGGCCTGGTGCTGCGCTCCCAGGACGTGGCCGGGATCCAGCAGGAGCTATGGGCTCATCTGACCGTCTACCAGGCACTGCGCC
GGGCGATGGTCGAGGCCGTCGAGACGCTTCCCGGCACGGATCCGGACAGGGCTTCCTTCACCGTCGCCCTGGAGACCGCCAAGGAACAGCTCATCACCGC
GGCCAATGTGCTGCCAGACGCCGGACCAGGGCGCATCACATCGGCCCTGCTGCACGATCTACTCCCACCCCGCCAGGCCCGCGTCAACCCGCGCCGCGTC
AAGTGCCCGATCTCCCGCTACGCCGCCCCGCCCGACCAGGCCCAAGCCCTCGGGGCCTCCAGGATCACCAGCATCGCCGTCACCGTGCACTCGTCCGCCG
GCACCGGCTCTGACGGGCGTCGCGACCACACGCTGCAACTCCTGCGCACCAACCCTCTCCGCACCTGGCGCGCATGCGAAATCGCCCGCGGTATCGGACT
GGACGACGCTCGAGGACTACGCGCGGAACTGGGGCGCTGGGTCCGCGAAGGAATCCTTCGCCGCACCGGGCGAGGCATCTACACCCTCGAACCCGAGTGG
ATCACACCCGACCTCCACCACCCCTCGGTCCCGCCCCATTTGACAACCGCGGATCGGCCTTAACTCCGTGGTCTTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1626 bp | 542 aa | 135 | 1760 | + | No |
Chemistry : DDE
ORF sequence :
MPVQCSTVTLTSSITVADGIFAPGHLGELTQQLPFELVDDVLERAGGAQHRLRLLPSRVGVYFVLALALFPQLGYVRVWDKLTAGLRGILHRRPSEKALR
EVRRRLGVAPLRLLFETLAGPVAQPITPGVRYRCWRTVAFDGCSSTKAPDRPRVCAWLGKHKHRYGTDGYPMLKIMVLCETGTRALLGAVFGPTPEKETG
YAEQLLPLLDGGMLLLNDRGFDSDDFLAKAAATGAQLLVRLKGTRTPARWALLPDGSFLTRINGTRLRVIDAHIAVTTAKGLRLEGHYRLATTLTDHRRY
PAVELVELYHERWEIESAFYSLRHTLQCGLVLRSQDVAGIQQELWAHLTVYQALRRAMVEAVETLPGTDPDRASFTVALETAKEQLITAANVLPDAGPGR
ITSALLHDLLPPRQARVNPRRVKCPISRYAAPPDQAQALGASRITSIAVTVHSSAGTGSDGRRDHTLQLLRTNPLRTWRACEIARGIGLDDARGLRAELG
RWVREGILRRTGRGIYTLEPEWITPDLHHPSVPPHLTTADRP
EVRRRLGVAPLRLLFETLAGPVAQPITPGVRYRCWRTVAFDGCSSTKAPDRPRVCAWLGKHKHRYGTDGYPMLKIMVLCETGTRALLGAVFGPTPEKETG
YAEQLLPLLDGGMLLLNDRGFDSDDFLAKAAATGAQLLVRLKGTRTPARWALLPDGSFLTRINGTRLRVIDAHIAVTTAKGLRLEGHYRLATTLTDHRRY
PAVELVELYHERWEIESAFYSLRHTLQCGLVLRSQDVAGIQQELWAHLTVYQALRRAMVEAVETLPGTDPDRASFTVALETAKEQLITAANVLPDAGPGR
ITSALLHDLLPPRQARVNPRRVKCPISRYAAPPDQAQALGASRITSIAVTVHSSAGTGSDGRRDHTLQLLRTNPLRTWRACEIARGIGLDDARGLRAELG
RWVREGILRRTGRGIYTLEPEWITPDLHHPSVPPHLTTADRP
Blast result :
Comments
ISSav1 is 41% aa similar to IS4.
ISSav1 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-76-D(N3)-97-E(C1). The copy number in Streptomyces avermitilis MA-4680 is 2.
ISSav1 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-76-D(N3)-97-E(C1). The copy number in Streptomyces avermitilis MA-4680 is 2.
References
1] Ikeda,H., Ishikawa,J., Hanamoto,A., Shinose,M., Kikuchi,H., Shiba,T., Sakaki,Y., Hattori,M. and Omura,S. (2003) Nat. Biotechnol. 21(5), 526-531.
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18