ISRba1
- Family IS4
- Group IS4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_005027 | ND | Rhodopirellula baltica | Rhodopirellula baltica SH 1 |
DNA section
IS Length : 1481 bp
Ends
IR Length : 16
IRL : GAATGGCACTTACTTTGCGCTAAATTCTTGACGAACTGCACTTTAGGCAC
IRR : GAATGGCACTTACTTTAGATCCGAGTCTCTCATTTTAGACGGTGGTTTCG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCCAACCGG | GGCTAACGCC | CATCGGCTAA | 10 |
| TTAGCCGATG | GGCGTTAGCC | CCGGTTGGCG | 10 |
| CGGTCCTCGG | GGCTGACACC | CCGAGCTACT | 10 |
| CATAGCTCGG | GGCGGAAGCC | CCGAGAACGC | 10 |
DNA sequence
GAATGGCACTTACTTTGCGCTAAATTCTTGACGAACTGCACTTTAGGCACATGGTTTGCGCATAGAGGCTTCTTTTCCCAATAAGGAGCCATTTTGGCAT
GAACTATACCGGTCCAAATCACGAAGCTGACCTCCAGTTCGACCGCTCTTTTGAGTTGTTGCAGCAGCTAGTCAACTTTGAAGATGCCAACAAGCTCTTC
GAGCAACAAGCTCATACCGTCTATACGGCATGTGTTGTCCTGTGGATGCTCGTTTACCAGCGACTCAAGCCGGATGCCTCGCTTGAAAACGCAGTCAAGC
ATTTACTCGATACGCGACCGACTTATCTTCCCGAAAACAAACGCCTGGAAGACAACACGCTCTCGGTCGCAACGGGTGGCTACAGTCGTGCTAGATCTCG
CTTACCGTTAGAAGTCGTTCGTTGGTTTGCCGAGGAAGTGAGCTCGGGGATCCTTTCTGCCACCGAGCCGGCGGTGGACGAACAACGCGTGTTCTTGATC
GATGGAACCACGTTGGCACTGGCCCCGGAAAAAGAACTCCAGCAAGCGTTTCCACCGGCAAGCAATCAGCTCGGTGAAGGCGTGTGGCCTTGTGTGCTGT
TGACGGTCTTTCACGAACTCGCCAGCGGTGCAGCGATGCTTCCACAAGTGGGACCGATGTACGGTCCTGAGGCGATCTCCGAAACGCAACTTGCTCGGCA
GGGGTTTGAGCAGCTTCCGGAGAATTCGATCATCATGTCCGATGCAGGATTTGGGATCTTTGGAATCGCACATGGGGCAATCGACGCAGGTCACGACATC
CTGCTGCGAATGAAGAAGGTGAACTTTCAGTCGCTGCAGAAGGATGCCGAATTGATCGAACAATCCGAGCACCACAAAACTTATCGGCACACCTGGAAAC
CCACGAAGAAGAATCGCCAGACGCAGCCAGATCTCCCGTCCGACTGCCAGTTGGATGTCTATTTGCATGAAGTCCAGGCGACCGACACGCTGACACTTTA
TCTGGTCAGTACGCTTGCGCAAGATGCTTTCGCGTCTGCCTCGCTATTTGAGCGACGCTACGACGTAGAAATTGACATTCGCAACTTCAAGGTGGTCATG
GACGCCGAGAACATTCGCGCCAAAAGCGTCGACACATTCATGAAAGAACTCTATACGTCGGTGGTGGCTTACAACCTGACCAGCCAGCTTCGGATCGAAG
CGGCGGCCTTCGAGAAAGTTCCGCCGCGTCGAATGAGCTTCAAACGAACGTGGACCACGTTTCAAACCTTCCTCCTGCGGCACATGCACACGGAGCCTGA
GCGTTGGAGAATGGCCTACCAAACAGCATTGTCGATCGCCCGCAAGGACAAGCTACCGATTCGCAGCAAACGTAGTTATCGCCGCGAGGCCTACCGGAAA
CGCCCCAAAAACATGCAGTTTGAAAAACGCACGAAACCACCGTCTAAAATGAGAGACTCGGATCTAAAGTAAGTGCCATTC
GAACTATACCGGTCCAAATCACGAAGCTGACCTCCAGTTCGACCGCTCTTTTGAGTTGTTGCAGCAGCTAGTCAACTTTGAAGATGCCAACAAGCTCTTC
GAGCAACAAGCTCATACCGTCTATACGGCATGTGTTGTCCTGTGGATGCTCGTTTACCAGCGACTCAAGCCGGATGCCTCGCTTGAAAACGCAGTCAAGC
ATTTACTCGATACGCGACCGACTTATCTTCCCGAAAACAAACGCCTGGAAGACAACACGCTCTCGGTCGCAACGGGTGGCTACAGTCGTGCTAGATCTCG
CTTACCGTTAGAAGTCGTTCGTTGGTTTGCCGAGGAAGTGAGCTCGGGGATCCTTTCTGCCACCGAGCCGGCGGTGGACGAACAACGCGTGTTCTTGATC
GATGGAACCACGTTGGCACTGGCCCCGGAAAAAGAACTCCAGCAAGCGTTTCCACCGGCAAGCAATCAGCTCGGTGAAGGCGTGTGGCCTTGTGTGCTGT
TGACGGTCTTTCACGAACTCGCCAGCGGTGCAGCGATGCTTCCACAAGTGGGACCGATGTACGGTCCTGAGGCGATCTCCGAAACGCAACTTGCTCGGCA
GGGGTTTGAGCAGCTTCCGGAGAATTCGATCATCATGTCCGATGCAGGATTTGGGATCTTTGGAATCGCACATGGGGCAATCGACGCAGGTCACGACATC
CTGCTGCGAATGAAGAAGGTGAACTTTCAGTCGCTGCAGAAGGATGCCGAATTGATCGAACAATCCGAGCACCACAAAACTTATCGGCACACCTGGAAAC
CCACGAAGAAGAATCGCCAGACGCAGCCAGATCTCCCGTCCGACTGCCAGTTGGATGTCTATTTGCATGAAGTCCAGGCGACCGACACGCTGACACTTTA
TCTGGTCAGTACGCTTGCGCAAGATGCTTTCGCGTCTGCCTCGCTATTTGAGCGACGCTACGACGTAGAAATTGACATTCGCAACTTCAAGGTGGTCATG
GACGCCGAGAACATTCGCGCCAAAAGCGTCGACACATTCATGAAAGAACTCTATACGTCGGTGGTGGCTTACAACCTGACCAGCCAGCTTCGGATCGAAG
CGGCGGCCTTCGAGAAAGTTCCGCCGCGTCGAATGAGCTTCAAACGAACGTGGACCACGTTTCAAACCTTCCTCCTGCGGCACATGCACACGGAGCCTGA
GCGTTGGAGAATGGCCTACCAAACAGCATTGTCGATCGCCCGCAAGGACAAGCTACCGATTCGCAGCAAACGTAGTTATCGCCGCGAGGCCTACCGGAAA
CGCCCCAAAAACATGCAGTTTGAAAAACGCACGAAACCACCGTCTAAAATGAGAGACTCGGATCTAAAGTAAGTGCCATTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1371 bp | 457 aa | 99 | 1469 | + | No |
Chemistry : DDE
ORF sequence :
MNYTGPNHEADLQFDRSFELLQQLVNFEDANKLFEQQAHTVYTACVVLWMLVYQRLKPDASLENAVKHLLDTRPTYLPENKRLEDNTLSVATGGYSRARS
RLPLEVVRWFAEEVSSGILSATEPAVDEQRVFLIDGTTLALAPEKELQQAFPPASNQLGEGVWPCVLLTVFHELASGAAMLPQVGPMYGPEAISETQLAR
QGFEQLPENSIIMSDAGFGIFGIAHGAIDAGHDILLRMKKVNFQSLQKDAELIEQSEHHKTYRHTWKPTKKNRQTQPDLPSDCQLDVYLHEVQATDTLTL
YLVSTLAQDAFASASLFERRYDVEIDIRNFKVVMDAENIRAKSVDTFMKELYTSVVAYNLTSQLRIEAAAFEKVPPRRMSFKRTWTTFQTFLLRHMHTEP
ERWRMAYQTALSIARKDKLPIRSKRSYRREAYRKRPKNMQFEKRTKPPSKMRDSDLK
RLPLEVVRWFAEEVSSGILSATEPAVDEQRVFLIDGTTLALAPEKELQQAFPPASNQLGEGVWPCVLLTVFHELASGAAMLPQVGPMYGPEAISETQLAR
QGFEQLPENSIIMSDAGFGIFGIAHGAIDAGHDILLRMKKVNFQSLQKDAELIEQSEHHKTYRHTWKPTKKNRQTQPDLPSDCQLDVYLHEVQATDTLTL
YLVSTLAQDAFASASLFERRYDVEIDIRNFKVVMDAENIRAKSVDTFMKELYTSVVAYNLTSQLRIEAAAFEKVPPRRMSFKRTWTTFQTFLLRHMHTEP
ERWRMAYQTALSIARKDKLPIRSKRSYRREAYRKRPKNMQFEKRTKPPSKMRDSDLK
Blast result :
Comments
ISRba1 is 39% aa similar to IS1271.
ISRba1 was found by screening completely sequenced bacterial genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-79-D(N3)-108-E(C1). The copy number in Rhodopirellula baltica SH 1 is 4.
ISRba1 was found by screening completely sequenced bacterial genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-79-D(N3)-108-E(C1). The copy number in Rhodopirellula baltica SH 1 is 4.
References
1] Gloeckner,F.O., Kube,M., Bauer,M., Teeling,H., Lombardot,T., Ludwig,W., Gade,D., Beck,A., Borzym,K., Heitmann,K., Rabus,R., Schlesner,H., Amann,R. and Reinhardt,R. (2003) Proc. Natl. Acad. Sci. U.S.A. 100(14), 8298-8303.
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18