ISPosp1
- Family IS4
- Group IS4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007948 | ND | Polaromonas sp. | Polaromonas sp. JS666 |
DNA section
IS Length : 1529 bp
Ends
IR Length : 17/20
IRL : GAATGGCACTTACATAAGCTGCATGACGTTGAAGTCACGTGCGTAAGCAC
IRR : GAATGGCACTTACTTTGGCTCCCAGTCGTGACCATGTATCTTGATTTTCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCACGCAATG | CCCTTAAGGC | CACGTAGCAT | 10 |
| GCTTGGCGAA | CGTTTAAGGG | ACGTTACCGT | 10 |
DNA sequence
GAATGGCACTTACATAAGCTGCATGACGTTGAAGTCACGTGCGTAAGCACGTGAATTTGAATGCAAAAGGAGGTGGCTGACCCGTGGATTGATAGGATGG
TTGTTACTACGCACCCAAACCATCTTTCCGAAGGCCAACCACCATGGATCGTAACGCCCGAAACGCTTTTCATCAACGTCAAGTCCGTATCAAACGCCAG
ACCAAGGCGACGCATGCGGTGGAGTTCTTCAACGTACTGACGAGTCCTGAGTTGCTGGAGACGACCGAGGCGCTGCTGCCCGAGCACCGCGAGCGGCTGT
ATCCGCCCACGGTTGCACTGTCGATGTTCATGCGCCAGGTGCTGGAGGCTGATGGCTCGTGCCAGAAGGCGGTCAATGGCTGGGCCGCGCAACGCGCGGC
CGATGGTTTGAGGCCATGCAGCGTTCGTACCGGCGGGTACTGCCGGGCTCGCCAGCGCCTGCCGCTGGAGATGGTCGGTACGCTGACACGCGAAACAGGC
CGCTTGCTCCACGAGAAGGCGCTGGCGCAATGGCTATGGCGTGGGCGCGCGGTCAAGCTGGTGGACGGCACCGGCATCTCGATGCCCGATACTCCCGAGA
ACCAGGAACGTTATCCCCAGCCCAGCACCCAGGCGCCTGGAGTAGGCTTTCCGCTGGCGCGACTGGTCATGGTGATTTGTCTGGCAACCGGCGCGGCGCT
GGATATGGCGGTAGGGCCACACAGCGGCAAGGGCAGCGGCGAACTCGGGCTTGTGCGCCGTCTGCTCGCAGGCTTCTGCCCAGGTGATGTGATGCTTGCC
GATGCCCTGTACTGCAACTATTTCTTGATCGCCTCCCTGATGGCCGCAGGCGTGGATGTGTTGTTCGAGCAAAACGGATCGCGCATCACCGACTTTCGCC
GTGGTCAGTCGCTGGGACCACGCGATCACATCGTGCGCTGGCCTAAACCGCCCCGCCCAGAGTGGATGACGCCTGAGCAGTACACGGGCTTTCCCGATGA
GCTAACGGTGCGCGAAGTCAAAGTAGCTCACCAGGTATTGGTCACCACACTGCTTGACCATCGCAAGGTCAGCAAGAACGATCTCTCGGCGCTGTACGCG
CGTCGCTGGAACGTCGAACTCGATCTGCGCAACCTCAAGACCACCACGGGCATGGATGTGCTGAGCTGCCAGACGCCGCAGATGAACGAAAAACAGCTAT
GGGTTCATCTGCTGGCCTACAACGTGATTCGGTTGCTCATGGCGCAGGCCGCCCGCAATGTCGGCATAGACCCGCGTGAGCTGAGCTTCAAGCACACCGT
ACAACTGTGGACCGAGTGGGTCTCGCGCGGTCTGTCGGCCACGAAGGAAGGCGGGCGGCTGTTCACATTGATCGCCCAGTGCAGGGTGGGCAATCGATCC
GCACGCATGGAGCCGCGAATGCGAAAGCGACGACCCAAACCCTACCCGTGGCTAAAGGTCCCGCGTGCGTATGCTCGCCGGAAAATCAAGATACATGGTC
ACGACTGGGAGCCAAAGTAAGTGCCATTC
TTGTTACTACGCACCCAAACCATCTTTCCGAAGGCCAACCACCATGGATCGTAACGCCCGAAACGCTTTTCATCAACGTCAAGTCCGTATCAAACGCCAG
ACCAAGGCGACGCATGCGGTGGAGTTCTTCAACGTACTGACGAGTCCTGAGTTGCTGGAGACGACCGAGGCGCTGCTGCCCGAGCACCGCGAGCGGCTGT
ATCCGCCCACGGTTGCACTGTCGATGTTCATGCGCCAGGTGCTGGAGGCTGATGGCTCGTGCCAGAAGGCGGTCAATGGCTGGGCCGCGCAACGCGCGGC
CGATGGTTTGAGGCCATGCAGCGTTCGTACCGGCGGGTACTGCCGGGCTCGCCAGCGCCTGCCGCTGGAGATGGTCGGTACGCTGACACGCGAAACAGGC
CGCTTGCTCCACGAGAAGGCGCTGGCGCAATGGCTATGGCGTGGGCGCGCGGTCAAGCTGGTGGACGGCACCGGCATCTCGATGCCCGATACTCCCGAGA
ACCAGGAACGTTATCCCCAGCCCAGCACCCAGGCGCCTGGAGTAGGCTTTCCGCTGGCGCGACTGGTCATGGTGATTTGTCTGGCAACCGGCGCGGCGCT
GGATATGGCGGTAGGGCCACACAGCGGCAAGGGCAGCGGCGAACTCGGGCTTGTGCGCCGTCTGCTCGCAGGCTTCTGCCCAGGTGATGTGATGCTTGCC
GATGCCCTGTACTGCAACTATTTCTTGATCGCCTCCCTGATGGCCGCAGGCGTGGATGTGTTGTTCGAGCAAAACGGATCGCGCATCACCGACTTTCGCC
GTGGTCAGTCGCTGGGACCACGCGATCACATCGTGCGCTGGCCTAAACCGCCCCGCCCAGAGTGGATGACGCCTGAGCAGTACACGGGCTTTCCCGATGA
GCTAACGGTGCGCGAAGTCAAAGTAGCTCACCAGGTATTGGTCACCACACTGCTTGACCATCGCAAGGTCAGCAAGAACGATCTCTCGGCGCTGTACGCG
CGTCGCTGGAACGTCGAACTCGATCTGCGCAACCTCAAGACCACCACGGGCATGGATGTGCTGAGCTGCCAGACGCCGCAGATGAACGAAAAACAGCTAT
GGGTTCATCTGCTGGCCTACAACGTGATTCGGTTGCTCATGGCGCAGGCCGCCCGCAATGTCGGCATAGACCCGCGTGAGCTGAGCTTCAAGCACACCGT
ACAACTGTGGACCGAGTGGGTCTCGCGCGGTCTGTCGGCCACGAAGGAAGGCGGGCGGCTGTTCACATTGATCGCCCAGTGCAGGGTGGGCAATCGATCC
GCACGCATGGAGCCGCGAATGCGAAAGCGACGACCCAAACCCTACCCGTGGCTAAAGGTCCCGCGTGCGTATGCTCGCCGGAAAATCAAGATACATGGTC
ACGACTGGGAGCCAAAGTAAGTGCCATTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1374 bp | 458 aa | 144 | 1517 | + | No |
Chemistry : DDE
ORF sequence :
MDRNARNAFHQRQVRIKRQTKATHAVEFFNVLTSPELLETTEALLPEHRERLYPPTVALSMFMRQVLEADGSCQKAVNGWAAQRAADGLRPCSVRTGGYC
RARQRLPLEMVGTLTRETGRLLHEKALAQWLWRGRAVKLVDGTGISMPDTPENQERYPQPSTQAPGVGFPLARLVMVICLATGAALDMAVGPHSGKGSGE
LGLVRRLLAGFCPGDVMLADALYCNYFLIASLMAAGVDVLFEQNGSRITDFRRGQSLGPRDHIVRWPKPPRPEWMTPEQYTGFPDELTVREVKVAHQVLV
TTLLDHRKVSKNDLSALYARRWNVELDLRNLKTTTGMDVLSCQTPQMNEKQLWVHLLAYNVIRLLMAQAARNVGIDPRELSFKHTVQLWTEWVSRGLSAT
KEGGRLFTLIAQCRVGNRSARMEPRMRKRRPKPYPWLKVPRAYARRKIKIHGHDWEPK
RARQRLPLEMVGTLTRETGRLLHEKALAQWLWRGRAVKLVDGTGISMPDTPENQERYPQPSTQAPGVGFPLARLVMVICLATGAALDMAVGPHSGKGSGE
LGLVRRLLAGFCPGDVMLADALYCNYFLIASLMAAGVDVLFEQNGSRITDFRRGQSLGPRDHIVRWPKPPRPEWMTPEQYTGFPDELTVREVKVAHQVLV
TTLLDHRKVSKNDLSALYARRWNVELDLRNLKTTTGMDVLSCQTPQMNEKQLWVHLLAYNVIRLLMAQAARNVGIDPRELSFKHTVQLWTEWVSRGLSAT
KEGGRLFTLIAQCRVGNRSARMEPRMRKRRPKPYPWLKVPRAYARRKIKIHGHDWEPK
Blast result :
Comments
ISPosp1 is 45% aa similar to IS4.
ISPosp1 was found by screening completely sequenced genomes for sequences homologous to the ISPpu8 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-78-D(N3)-104-E(C1).The copy number in the chromosome of Polaromonas sp. JS666 is 2.
ISPosp1 was found by screening completely sequenced genomes for sequences homologous to the ISPpu8 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-78-D(N3)-104-E(C1).The copy number in the chromosome of Polaromonas sp. JS666 is 2.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Munk,A.C., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Anderson,I. and Richardson,P. (2006) Unpublished
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Munk,A.C., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Anderson,I. and Richardson,P. (2006) Unpublished