ISPa1635

  • Family IS4
  • Group IS4
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AY539834 ND Pseudomonas aeruginosa
Pseudomonas aeruginosa isolate 290
DNA section
IS Length : 1635 bp

Ends


IR Length : 14/15

IRL : CAATGTCATCAACTTTGGAAATTATCCATAAATATCATATAATTAGCGCT
IRR : CAATGTCATCAACCTAAGCTGCAGCCCTGCCCAAGGCAAGCCCCCCGAAG

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNNNGGCGGTCGGTNNNNNNNNNN10

DNA sequence

CAATGTCATCAACTTTGGAAATTATCCATAAATATCATATAATTAGCGCTCAAATCAGTGCATGGGAGAAGCCCTTTTGACTACCTATACCGCATCGCTT
GACACCGCCAGCCAAACCCTGGCTTGAAAATTGTACAAAAAATAACCAGCCCGCTCGATTGTCCCGCCTTCATCGCCGCCCACCGCCAGAATCCTCAAGA
CTTTACCCGCCGACGCCAGCTCACCTTCAAGAACCTCGTCCTGTTCCTGCTCAATCAGCCGCGCACGGCCCTGCAAACCGAACTCGATCAGTTCTATCGC
GTACTCAATCAGGCGTCGACTGAGACGCAAATGGTCACTGCGCAGGCCTTCTGCAAAGCGCGCAAAAAGCTCAACCCCGAGGTATTTGAAAGTCTCAATC
GCCTCCTGCAGCAACAAATTGACTGCTTCGGGCTGCGTCAGAAGTGGCGTGGGCTGAGGGTGCTCGCGGTGGATGGCTCGACCGTGCATTTACCCCTGGA
GTCGACCATGGCGACCTTCTTTGGCAGTCACTCTGGCTTTCCCATGGCTCGCTTGTCCACACTCTACGAAGTCGCTGACGGTCAAACCTTGCATAGCCTG
ATCGTGCCCCTGACCGTCGGCGAGCGCGACTGTGCCCATCTGCACCTTGAGCACCTGCCGGCTGACAGCCTGACGCTGTTTGACCGTGGTTACCCTGGGC
ACTGGTTATTCGCGCTGTTCGCGCAGCAACAGCGGCACTTTCTGATGCGCCTGCCCTGTGGCTATAACGCCCAGGTCAAAGCGTTTCTACACTCTGGCCA
AGTTGAAGACACACAGCTTTTCGTGGCCAACCATCCTGAAGCACGTCTGTTCTGCTCCGAGGCTGGCGTTGATCCTGCCAGCCAGATCGAGCTGAGACTG
ATCCGGGTCGAGTTGGCCAACGGCGAAAGCGAAGTATTGCTGACCTCGCTGCTGGATCGAGAAGCCTTTCCCGCTGAGGTGTTTGCCGAGCTTTACCACC
GCCGCTGGGGCATCGAAACCGATTTCCGTCGCCTCAAACAAACCCTGACGCTGGACAACTTCAGCGGCCGCAGCGTGACCGCTGTAAAGCAGGACTTTCA
TGCCGCTCAACTGTTGAAGAACCTGGCGCTGTTGATGCAGCACCTGCTACAGCCGGTCATCGAACAGCGCCACAAAGGTCGCAAGCTGCGATGGAAAGTC
AACTTCACCCAAGGCGTGTCACGGCTCAAAAATACCCTGGTCGAGCTGCTGGTGCGGCACTGTGCTCAGGGGCTGAGCAATGTGCTGGCCTTGATGGCTA
AAAGCCTCAGTGCCGTACGCTCAGGCCGTAGCTTTGCGCGCCAACGCAAACGCGCAGCCAGCCGAGGTTGTGAGGGCTACAAACCGACGCGCTGAAGAAA
CACCACCGCCCAACCTCCGTGCAAAAAAGCTGGCCACAAGCGGTCGGCATAGGCTCGTCCGGAGGACGAGAAATCTCGCCTAGACAGCCGGAACAGACGA
TGATCACATCACCGCCAGCCCAGCTAGACCGCCTGATTGAGCGGCAGAGGCCTGGTCATCGCCCGACGGCCATGGCGGCTGGCTGCTTCGGGGGGCTTGC
CTTGGGCAGGGCTGCAGCTTAGGTTGATGACATTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1062 bp354 aa3311392+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MVTAQAFCKARKKLNPEVFESLNRLLQQQIDCFGLRQKWRGLRVLAVDGSTVHLPLESTMATFFGSHSGFPMARLSTLYEVADGQTLHSLIVPLTVGERD
CAHLHLEHLPADSLTLFDRGYPGHWLFALFAQQQRHFLMRLPCGYNAQVKAFLHSGQVEDTQLFVANHPEARLFCSEAGVDPASQIELRLIRVELANGES
EVLLTSLLDREAFPAEVFAELYHRRWGIETDFRRLKQTLTLDNFSGRSVTAVKQDFHAAQLLKNLALLMQHLLQPVIEQRHKGRKLRWKVNFTQGVSRLK
NTLVELLVRHCAQGLSNVLALMAKSLSAVRSGRSFARQRKRAASRGCEGYKPTR

 

Blast result :
Comments
ISPa1635 is 74% aa similar to ISHch2. ISPa1635 caused carbapenem resistance through insertional inactivation of the oprD gene from Pseudomonas aeruginosa. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-69-D(N3)-110-E(C1).
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Wolter,D.J., Hanson,N.D. and Lister,P.D.(2004)FEMS Microbiol. Lett. 236 (1), 137-143