ISIlo1
- Family IS4
- Group IS4
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_006512 | ND | Idiomarina loihiensis | Idiomarina loihiensis L2TR |
DNA section
IS Length : 1440 bp
Ends
IR Length : 15/18
IRL : TAATGCCGATCAGTTAAGAAAAATTTTAGCGATCGGTTGACCGATCCGGC
IRR : TAATGCTTGTCAGTTAAGCTAACTGGCTGTCGGTTTAGAAGGCGGTCGCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTAAAAAGGC | TCCCGAAGGA | GCCTTTTAAA | 10 |
| ACAAAAAAGG | CGCCAAACGGC | GCCTTTTTTA | 11 |
| ATTAAAAGGC | CTCCCTTGCGG | AGGCTTTTTT | 11 |
| AACAAAACCC | GGCCTTCGCGCC | GGGTTTTTTG | 12 |
| AAAAAAACCC | GGCACGGTGGCC | GGGTTTCAGT | 12 |
DNA sequence
TAATGCCGATCAGTTAAGAAAAATTTTAGCGATCGGTTGACCGATCCGGCAGAGACTTCAAAATCCGTAGTTAGGTTTTCTAACTACGGATTTTTTATGT
TTCTGAGTCAAGCACTGGACCAACTGCAGGAGTTTACGCCGGATGATTTTAGTAAACTACCGGATCTGCTGGAGCCTGAGTTAATCGAACAATGCCTTGA
AGACGCGGGCGTTACAACGGTACGCAAACGCCGTTTGCCAATGGACTTTATGGTCTGGGCGGTCGTAGGAATGGCATTATTTCGGGAAATGCCGATGCGT
CAGCTCGTTTCACACCTTGATTTAATGTTGCCCGGCAAAAGACCTTACGTTGCGCCGAGTGCCGTAGTTCAGGCTCGTCAGCGTTTGGGGGCTAATGTTA
TGAAGCGTATGTTTGAGCTCACTAATGAGCTGTGGTTCGATAAACTACCGCTTTCTCACTGGAATAATCTATCGGTTCTGGCTGTCGACGGCACCGTCTG
GCGTACACCGGACACACCGGAGAATGATGCTTCCTATGGGCGAACGGCTAACCAGCGGGCTGAATCCGACTATCCTCAAATTCGGATGGTGAATCAAATG
GAAGTAAGCAGCCATTTAGTCACCTCGACGGAGTTCGGCAGTATTGCAGAGGTTAGTGAAGTAGACCTGGCAGCCCGGCTGATTGAACAGACGCCGGACC
ACTCACTGACGCTGTTCGATAAAGGGTTTTATGCGCTGGGTTTACTGGATCAATGGCATCGTGCCGGTGAGGAGCGCCATTGGCTTATTCCCCTCAGAAA
AGGCGCACAATACTCTGTCAAGAAGTCTTTTAGCGACACGGACAAATTGGTAGAAATTAGGCTCTCACCACAAGCTAAGAAGAAGTGGGATAATGCACCG
GACACTGTTCAGGCGCGTTTAGTGTGTAAGAAAATAGCTAAAAAAGAGGTTGCGATATTAACCTCAATGACAGACACGATGCGTTATCCTGAAGCCGATA
TCGTTGACTTATATGCTCATCGCTGGGAAATCGAGCAGGCTTACCGTGAAATGAAACAATATATGCTGAAGAGTGAGCTGACCCTGCGCAGCAAACGGCC
TGACCTGGTTGAGCAGGAGTTGTGGGGCATGCTGGTCGCTTATAACTTATTGCGGTATATGATGGCGCAAATGGCCTATAGCCTGAAGCATATTGAGCCG
AATCAAATAAGTTTCAAACAAGCCTCTCATTATCTGGTGGGGCAGTTACATATACTGGTACACATCTCACCCGGAAAAATACCCAAAGTCATGGAGCAAA
TGATGGAAATGGCCAGCGCATTCGTGCTGCCCGAAAGACGAGAGCGATATTACCCTCGTTCAATAAAAAGGCGGCCGCAACGTTATCCGATGCGACCGCC
TTCTAAACCGACAGCCAGTTAGCTTAACTGACAAGCATTA
TTCTGAGTCAAGCACTGGACCAACTGCAGGAGTTTACGCCGGATGATTTTAGTAAACTACCGGATCTGCTGGAGCCTGAGTTAATCGAACAATGCCTTGA
AGACGCGGGCGTTACAACGGTACGCAAACGCCGTTTGCCAATGGACTTTATGGTCTGGGCGGTCGTAGGAATGGCATTATTTCGGGAAATGCCGATGCGT
CAGCTCGTTTCACACCTTGATTTAATGTTGCCCGGCAAAAGACCTTACGTTGCGCCGAGTGCCGTAGTTCAGGCTCGTCAGCGTTTGGGGGCTAATGTTA
TGAAGCGTATGTTTGAGCTCACTAATGAGCTGTGGTTCGATAAACTACCGCTTTCTCACTGGAATAATCTATCGGTTCTGGCTGTCGACGGCACCGTCTG
GCGTACACCGGACACACCGGAGAATGATGCTTCCTATGGGCGAACGGCTAACCAGCGGGCTGAATCCGACTATCCTCAAATTCGGATGGTGAATCAAATG
GAAGTAAGCAGCCATTTAGTCACCTCGACGGAGTTCGGCAGTATTGCAGAGGTTAGTGAAGTAGACCTGGCAGCCCGGCTGATTGAACAGACGCCGGACC
ACTCACTGACGCTGTTCGATAAAGGGTTTTATGCGCTGGGTTTACTGGATCAATGGCATCGTGCCGGTGAGGAGCGCCATTGGCTTATTCCCCTCAGAAA
AGGCGCACAATACTCTGTCAAGAAGTCTTTTAGCGACACGGACAAATTGGTAGAAATTAGGCTCTCACCACAAGCTAAGAAGAAGTGGGATAATGCACCG
GACACTGTTCAGGCGCGTTTAGTGTGTAAGAAAATAGCTAAAAAAGAGGTTGCGATATTAACCTCAATGACAGACACGATGCGTTATCCTGAAGCCGATA
TCGTTGACTTATATGCTCATCGCTGGGAAATCGAGCAGGCTTACCGTGAAATGAAACAATATATGCTGAAGAGTGAGCTGACCCTGCGCAGCAAACGGCC
TGACCTGGTTGAGCAGGAGTTGTGGGGCATGCTGGTCGCTTATAACTTATTGCGGTATATGATGGCGCAAATGGCCTATAGCCTGAAGCATATTGAGCCG
AATCAAATAAGTTTCAAACAAGCCTCTCATTATCTGGTGGGGCAGTTACATATACTGGTACACATCTCACCCGGAAAAATACCCAAAGTCATGGAGCAAA
TGATGGAAATGGCCAGCGCATTCGTGCTGCCCGAAAGACGAGAGCGATATTACCCTCGTTCAATAAAAAGGCGGCCGCAACGTTATCCGATGCGACCGCC
TTCTAAACCGACAGCCAGTTAGCTTAACTGACAAGCATTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1323 bp | 441 aa | 97 | 1419 | + | No |
Chemistry : DDE
ORF sequence :
MFLSQALDQLQEFTPDDFSKLPDLLEPELIEQCLEDAGVTTVRKRRLPMDFMVWAVVGMALFREMPMRQLVSHLDLMLPGKRPYVAPSAVVQARQRLGAN
VMKRMFELTNELWFDKLPLSHWNNLSVLAVDGTVWRTPDTPENDASYGRTANQRAESDYPQIRMVNQMEVSSHLVTSTEFGSIAEVSEVDLAARLIEQTP
DHSLTLFDKGFYALGLLDQWHRAGEERHWLIPLRKGAQYSVKKSFSDTDKLVEIRLSPQAKKKWDNAPDTVQARLVCKKIAKKEVAILTSMTDTMRYPEA
DIVDLYAHRWEIEQAYREMKQYMLKSELTLRSKRPDLVEQELWGMLVAYNLLRYMMAQMAYSLKHIEPNQISFKQASHYLVGQLHILVHISPGKIPKVME
QMMEMASAFVLPERRERYYPRSIKRRPQRYPMRPPSKPTAS
VMKRMFELTNELWFDKLPLSHWNNLSVLAVDGTVWRTPDTPENDASYGRTANQRAESDYPQIRMVNQMEVSSHLVTSTEFGSIAEVSEVDLAARLIEQTP
DHSLTLFDKGFYALGLLDQWHRAGEERHWLIPLRKGAQYSVKKSFSDTDKLVEIRLSPQAKKKWDNAPDTVQARLVCKKIAKKEVAILTSMTDTMRYPEA
DIVDLYAHRWEIEQAYREMKQYMLKSELTLRSKRPDLVEQELWGMLVAYNLLRYMMAQMAYSLKHIEPNQISFKQASHYLVGQLHILVHISPGKIPKVME
QMMEMASAFVLPERRERYYPRSIKRRPQRYPMRPPSKPTAS
Blast result :
Comments
ISIlo1 is 70% aa similar to IS4.
ISIlo1 was found by screening completely sequenced genomes for seqences homologous to the IS4 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-76-D(N3)-104-E(C1). The copy number in Idiomarina loihiensis L2TR is 5.
ISIlo1 was found by screening completely sequenced genomes for seqences homologous to the IS4 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-76-D(N3)-104-E(C1). The copy number in Idiomarina loihiensis L2TR is 5.
References
1] Hou,S., Saw,J.H., Lee,K.S., Freitas,T.A., Belisle,C., Kawarabayasi,Y., Donachie,S.P., Pikina,A., Galperin,M.Y., Koonin,E.V., Makarova,K.S., Omelchenko,M.V., Sorokin,A., Wolf,Y.I., Li,Q.X., Keum,Y.S., Campbell,S., Denery,J., Aizawa,S.I., Shibata,S., Malahoff,A. and Alam,M.(2004)Proc. Natl. Acad. Sci. U.S.A. 101,18036-18041