ISfinder Mirror Site

ISFsp5

Family IS4
Group IS4

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Frankia sp.	Frankia sp. EAN1pec

DNA section

IS Length : 1617 bp

Ends

IR Length : 14/16

IRL : CAATGCCACTAAGTTAGAGGCCCGGTTCCGCCGTCAATCACGACACGCCA
IRR : CAATGCCGCTTAGTTAAGCCAGTCGCGCGAGCGTGATCGTCGGTGGTTGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCGGGTTCGG	GGTTCNAGTC	CCTCCGAGCG	10

DNA sequence

CAATGCCACTAAGTTAGAGGCCCGGTTCCGCCGTCAATCACGACACGCCAACGGACTATCATAGAATCGGCGCATTTCCCACGATGCGGAGAGCAGCTTC
TACAACATGCGCCGACAGTTCGTTGATCCCGGACGTCGCTAGAATGCCGGTGTGGCGCGTCTCCCGGGGGCTCTTGAACTGCCCGCAGTTACCAGCGACC
GACTGACAGATCGCATTTCTCTCGGCGTTCTCGCGCGTATCGTTCCTCGAGACCTGGTCGACGAGGTGCTCGCGGAGACGAGACGGCTGGAACAGCGGAA
GAGGCTGCTACCAGCGAGAGTCGTCGTGTATTTCACGATGGCCATGTGCCTGTTCTTCGACGACGACTACGACGAAGTGATGCGCAGGCTCGTGGGCACG
CTGCGCTGGCTCGGCTCCTGGAAAGGAGACTGGAAGGTCCCCAGCACCGGAGCGATCTCCCAGGCCCGGACACGTCTCGGCCCGGAACCGTTGAAGCTGC
TGTTCGAACGGGTCGCCGTGCCAGTCGCCGGCCTGGGCACGAAAGGCGCCTGGCTCGGCTCCCGCCGACTTGTCGCCGTGGACGGCGTCCACCTCGACAC
CGCGGACACCCCCGAGAACGCCGACGCGTTCGGCCGTTTCAGCCACGGACCGAAGACCGCCGCCTTTCCCCAGGTACACGTCGTCGCGCTCGCCGAATGC
GGTACCCATGCGGTCTTCGCCGCCGCCATCGGCGCCTACACCAGCGACGAACGGTCGCTCGCTGCCACGCTGTTCGACGCCTGCGAGCCGGGCATGCTCC
TCACCGCCGACCGCAACTTCTACGGCTACGGCCTGTGGCAACAGGCCCTCGCGACCGGTGCGGACCTGCTCTGGCGAGTCAACGCGAACCTCACGCTCCC
CGTCATCCGAGCCCTGCCCGACGGCTCCTACCTCTCTCTCCTGATCGACCCCAAGATCCCCGTCGCCAGACGTGGCCAGCTCATCGCCGACGCCCGTGCC
GGCCACGCCCCACCCACAGAGTCAGCCCTACCAGTCCGAGTCATCGAGTACTCCGTACCAGACCACGAAGAAAACGGCACGAGCGAACTCATCTGTCTGA
TCACCAACATCCTCGACCCCACCGACGTGGCCGCCATCGAACTCGCGACCGCCTACCACGAACGATGGGAAATAGAAAGCACCTTCGATGAAATCAAAAC
GCACCAGCGAGGCGAGAAACGGGTCCTACGCTCCAAAAATCCCGAACTCGTCAAACAAGAGATCTGGGCCCTCCTGCTCACGCACTACGCCATACGAAGC
CTCATGATCGAGGCCGCCGACGAGGCCGATATCGACCCCGACAGACTCTCCTTCATGAGAACACTACGGATCACACGCCGCCAGGTCACCGACCAGGCGG
ATTTTTCCCCCTGACCGCCACACCCGCGCGTGGGAGCAGACCCGCACCGAGATCCTCGAACGGATCAACCCCGCCCGCCGCCACCGCACCTACCCGCGCG
CTGTCCGACGAACCAGGTACAACGCCTACCGGATCAAAAAACCAACGGACAGCGGAACCCACCACCGCCAACCACCGACGATCACGCTCGCGCGACTGGC
TTAACTAAGCGGCATTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1260 bp	420 aa	152	1411	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARLPGALELPAVTSDRLTDRISLGVLARIVPRDLVDEVLAETRRLEQRKRLLPARVVVYFTMAMCLFFDDDYDEVMRRLVGTLRWLGSWKGDWKVPSTG
AISQARTRLGPEPLKLLFERVAVPVAGLGTKGAWLGSRRLVAVDGVHLDTADTPENADAFGRFSHGPKTAAFPQVHVVALAECGTHAVFAAAIGAYTSDE
RSLAATLFDACEPGMLLTADRNFYGYGLWQQALATGADLLWRVNANLTLPVIRALPDGSYLSLLIDPKIPVARRGQLIADARAGHAPPTESALPVRVIEY
SVPDHEENGTSELICLITNILDPTDVAAIELATAYHERWEIESTFDEIKTHQRGEKRVLRSKNPELVKQEIWALLLTHYAIRSLMIEAADEADIDPDRLS
FMRTLRITRRQVTDQADFSP

Blast result :

Comments

ISFsp5 is 61% aa similar to ISMfl1. ISFsp5 was found by screening completely sequenced genomes for sequences homologous to the ISRso13 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-75-D(N3)-121-E(C1). The sequencing of the Frankia sp. EAN1pec chromosome is currently in progress. Accession number: NZ_AAII00000000.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2005) Unpublished.