ISfinder Mirror Site

ISBlma2

Family IS4
Group IS4

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Blastopirellula marina	Blastopirellula marina DSM 3645

DNA section

IS Length : 1439 bp

Ends

IR Length : 21

IRL : GAACGCCGTTGCGCATCTGGGTAGGCGACAAACTCTAAAATTGATCTAGC
IRR : GAACGCCGTTGCGCATCTGGGCTATGGCGGCCGCATAAACTGTTGGGCCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGATGGCATG		CGTGCCATGC	0
GGGTGGCACG		AACTATTCCT	0

DNA sequence

GAACGCCGTTGCGCATCTGGGTAGGCGACAAACTCTAAAATTGATCTAGCAAAGAACTTCGAGCCTGTGCTTCAAGGGAGTTGGACGACTTCCACAAGCA
GCAGGGAGGCTCGAAGGTGGCCCGAGGACCACATCGCGTATGCTATCAGCGCGAGCTGACGGCGGCGATATCGCAATTCTTGCCGCCGCAATTTTTTGCG
CGCTGGCGATTTCATGGCAACGCAAATTGGACGCCTCAGCGGTTGGCTTGGGCGGCATGCCTGATGAGTTGGAGCACCGATTCCACACTCACCGATCGAC
TGGAGAGCGTCGGCGCTTTACTGGGCGAACTCTTTCCGCGTTGGCGCGTCGGCTTGGCGTTGGGCGGATTCGGCCGCGCATGCATTCGTGAGACGCCGCG
GATGCTTGACGAAGTGCGTCGCCAACTGCGCAGTTCGGTCGCTGGTTGGTTGGAGCAATATCGCGTGTTCGGTTGGGTGGTGATGGCGGTCGACGGTTCG
CGCTTCGAGGCTCCGCGTACGCGCGCCAACGAAGCAGGCCTCGGCTGCGCAGGACGCGAGAAGACGACGCCGCAAATTTATCAAACCACGTTGCAGCATG
TCGGCACGTCGTTGCCGTGGGACTTTCGCATCGGCCCCGGCACGGCCAGCGAACGACGTCAGTTAGACGAAATGCTCCCTGATTTACCGGGTAAATCGCT
GCTAATCGCCGACGCCGGCTTCATCAGTTACGATCTCTGTCGCGTGCTGCTGATGGGGCGGCATGACTTTCTACTGCGCGTCGGCGGAAATACGCACCTG
CTGGAAAAGCTCGGCTTCGCTTGCGAAACGCGCGAGCGAACCGTCTATCTATGGCCGTTGAGATTTCATGCGATTCCGCCGGTCGTGTTGCGACTGATCG
TGCTCCGCGACGCGAACAAAGAACCGGTTTATCTGGTGACAAATATTGATTCGGAGTCGCTTCCCGAAGAAATCGCGAGTCAAATTTATCGTCTTCGCTG
GGGACTCGAAACTCACTTCCGCGGGTTGAAACAAACGCTTGGTCGCGATCGCGTGTTAAGTCGAACTCCAGCCACGGCGCTCGCCGAACAGGGCTGGCTC
AACATCGGGGCTTGGTTGCTGCAGTTGATGACGACCGCCGCGCTGATCGCGGCGAACGAACAGCCGCGGCAATGGTCGGTAGCCAAGGCAAGTCGCGAAG
TTCGCCGCACGATGCGCGGCTGTCCGTCACGCTGTATCCGCCAACCGCTCTGCCAACGCTTGGGCTGCGCCAAGATCGACGGATACCAACGAAAAGGCCC
CAAACAAACCCGCCGTGGCGTGCAAAAGAAGCGAGAAAAGCCGCCAGGACCACCGAAAATACGACAGGCTACGAAGAAGGAACAACAACTGGCCCAACAG
TTTATGCGGCCGCCATAGCCCAGATGCGCAACGGCGTTC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1152 bp	384 aa	264	1415	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSWSTDSTLTDRLESVGALLGELFPRWRVGLALGGFGRACIRETPRMLDEVRRQLRSSVAGWLEQYRVFGWVVMAVDGSRFEAPRTRANEAGLGCAGREK
TTPQIYQTTLQHVGTSLPWDFRIGPGTASERRQLDEMLPDLPGKSLLIADAGFISYDLCRVLLMGRHDFLLRVGGNTHLLEKLGFACETRERTVYLWPLR
FHAIPPVVLRLIVLRDANKEPVYLVTNIDSESLPEEIASQIYRLRWGLETHFRGLKQTLGRDRVLSRTPATALAEQGWLNIGAWLLQLMTTAALIAANEQ
PRQWSVAKASREVRRTMRGCPSRCIRQPLCQRLGCAKIDGYQRKGPKQTRRGVQKKREKPPGPPKIRQATKKEQQLAQQFMRPP

Blast result :

Comments

ISBlma2 is 38% aa similar to IS4. ISBlma2 was found by screening completely sequenced genomes for sequences homologous to the ISPa12 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-72-D(N3)-98-E(C1). The sequencing of the Blastopirellula marina DSM 3645 chromosome is currently in progress. Accession number: NZ_AANZ00000000.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Amann,R., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Unpublished