ISRsp6

  • Family IS4
  • Group IS4Sa
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
U00090 ND Rhizobium sp.
Rhizobium sp. NGR234
DNA section
IS Length : 1277 bp

Ends


IR Length : 19/20

IRL : CAGGGCTGTCCGGGGAAAGTTGCGGCTGAATCGAAATGTCGCAGGCGCAT
IRR : CAGGGCTGTCCGGGGAAATTCAGACATAGGCGAAGCTCATTTGATGGGGG

Insertion site


Left flankDirect repeatRight flankDR Length
TATCTCCTCCCGCCGCGGCGGGAGGATATA10

DNA sequence

CAGGGCTGTCCGGGGAAAGTTGCGGCTGAATCGAAATGTCGCAGGCGCATGCCCCGTCAGACGGGGATTTTCCGTCTACCTTCTGGTTGTCGAGACTCAG
AAAGAGGACGGGGCATGCGGTTCACACGTAGCATTCTTGGCAAGCTGATTGAACCGGTCAATCGGCGCCGCTTCCAGACGATAGTGGATGATCACGACGG
GGACGCCTACGACAAGGCGTTCCGCAGCTGGGACTGCTGGTGCTGATCTATGCCCAGTTGAGCGGTGCGGAGAGCCTGCGCGGCCTGGAGGCCGGCTGGA
ACGCCAACAGCCATCACCACTATCACCTGGGCAGCGACCGCTTGCCGATGCCAACGCCCGCCGCCCGGTTGCCGTCTTTGCCGAGACCTTCGGCCTGCTT
GCCGGCCAACTCGACCGACAGACGCGCCGCGAGGGCCGGGCCATGCTGCGGCTGATCGATTCCACCCCGATCCCGCTCGGCAAGCTGTGCGGCTGGGCCA
AGTCGAACGGCCGCATCCGCGGCATGAAGATGCATGTCGTCTATGACCCCGACAGCGACTGCCCGCGCCTCCTCGACATCACCGATGCCAACGTCAACGA
CGCCCAGATCGGTCGCACCATCGCCATCGAGAGCGGCGCCACCTATATCTTCGACAAGGGCTATTGCCACTACGGCTGGTGGACGGCGATCGCCGAGGCG
AAGGCGTTCTTCGTGACCCGGCCGAAGTCCAACATGGGCCTCAAGGTGGTGCGCCAACGGCGCATCAAGGTCGCCGAGGGCGACGGCTTCACCGTCATCG
ACGACGCCACCGTGCGCCTGGCCAGCAAGGGTGATTCCAAGCTGCCGATCCCGTTGCGCCGCCTCACCGTCAAGCGCGCCGACGGCGACACCATCACGCT
GCTGACCAATGATCGCAAGCGGCCCGCCGTGGCCATCGCGGCGCTCTACAAAGGCCGCTGGCAGATCGAACTCCTGTTCCGCTGGATCAAGCAGCACCTC
AAGATCCGCAGTTTCCTTGGCAACAACGACAACGCCGTGCGCCTGCAGCTCTTCGCCGCGATGATCGCCTATGCGCTACTGCGCATCGCGGCGCGCCTTA
ACCGCATCACCATGCCGATCCTGCGCTTCACCGACTTGGTTATCCGCTGCCTCTTCGAGCGCCGCGACATCGCCGCCATCGAGCGGCCGCCACCGGTCAA
TCCCAGCCACAGGAGACCCCGGTGCTCCCCCCATCAAATGAGCTTCGCCTATGTCTGAATTTCCCCGGACAGCCCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1068 bp356 aa1881255+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MITTGTPTTRRSAAGTAGADLCPVERCGEPARPGGRLERQQPSPLSPGQRPLADANARRPVAVFAETFGLLAGQLDRQTRREGRAMLRLIDSTPIPLGKL
CGWAKSNGRIRGMKMHVVYDPDSDCPRLLDITDANVNDAQIGRTIAIESGATYIFDKGYCHYGWWTAIAEAKAFFVTRPKSNMGLKVVRQRRIKVAEGDG
FTVIDDATVRLASKGDSKLPIPLRRLTVKRADGDTITLLTNDRKRPAVAIAALYKGRWQIELLFRWIKQHLKIRSFLGNNDNAVRLQLFAAMIAYALLRI
AARLNRITMPILRFTDLVIRCLFERRDIAAIERPPPVNPSHRRPRCSPHQMSFAYV

 

Blast result :
Comments
ISRsp6 is 85% aa similar to ISRm16. ISRsp6 was found by screening completely sequenced genomes for sequences homologous to the ISRso13 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-64-D(N3)-105-E(C1). The copy number on plasmid pNGR234a from Rhizobium sp. NGR234 is 1.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Freiberg,C., Fellay,R., Bairoch,A., Broughton,W.J., Rosenthal,A. and Perret,X.(1997)Nature 387 (6631), 394-401