ISPto2

  • Family IS4
  • Group IS4Sa
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Psychroflexus torquis
Psychroflexus torquis ATCC 700755
DNA section
IS Length : 1389 bp

Ends


IR Length : 17

IRL : CACTAGCATCCGAAAGTCGGGATTTTAAAAAGCCCTCAGAATCACGTATT
IRR : CACTAGCATCCGAAAGTTTTTAGAAAGAAGTAAGAAACATGTTTCTACCC

Insertion site


Left flankDirect repeatRight flankDR Length
TGATGTAGACTTAAAATATAACAAACTGG9

DNA sequence

CACTAGCATCCGAAAGTCGGGATTTTAAAAAGCCCTCAGAATCACGTATTTTTGAGTTCTACCAAAAACTTTTCAAATATGGGACTCTTCAGGCGAAACA
AAAATAACAATAAACCAGTCATTCGACAAATTTTAGATTTAGTACCCCATTGGTTATTTAGAAGTTGCACCAATACCTACAAAACAGATAAAGGTGTTCA
TAAGTATAGAACTTACGACCAGTTTGTTGCTTTAACTTTCGGACAGCTTAATAAGTGCCAGAGCCTCAATGATATATCAGCAGGAATTGGAGTTAGCGAG
ATTTTTATAAGCGACTTAGGTTTAACCCAAAGCCCTGCTCGTTCTACAATGAGCGATGGCAATAAAAAGCGTGACTGGCAGGTGTTTGAGAGCTTGTATT
ATCGTTTATTAAGTCACTATAAATCGGTGCTAAAACAGCATCATAACACCCATATTATAGAAGAGATTAAGGGCAAGGTAGTTAAACTAATAGATAGCTC
AACGATTTCCTTGTGTCTGGCTATGTTTGATTGGGCAGAGTTTCGGACAGCTAAAGGAGGTATTAAATTGCACACTAGCTGGGATTATAATTTAATGATA
CCTGATGTAGTAAATATAACAGAAGCAAAGGTTCATGACAGATATGGATTAAAGCAACTGATTTTCCCTAAAGACACTATTATAGTTGAAGACAGAGCTT
ATTTCGATTTTGAATTAATGCTCAATAGAATAAAAGCAGAAAATGTATTTGTTACTAGAATTAAGTCCAACACTTTGTATGAAACGATAGAGGAGCTGGA
GCTTGCTGATGATGTTGACCAACATATTCTTAAAGATGAAATAATACAACTTACATCAGGTAGGGCGATAGAAACGGGTATTTCAAAGCATAAACTAAGA
TTGGTACATGTTTATAAAGAAGATGAAAACAAAGTAATTGCCATTATTACCAACCAACTTGATTGGGAATACAATACCATTGCAGCATTATATAAGAAAC
GTTGGGATATTGAATTGTTTTTTAAAGCTTTAAAACAAAACCTACAAGTAAAAACCTTCTGGGGAACCAGTGAGAATGCAGTAAAATCGCAAATATATGT
AGCGCTGATAAATTATCTACTTTTAGAGCTTATAAAGCGAACTATTTCCAAAAAAGCAGTTGCTTTTTCTAATTTGTCAGAAAAAGTGAGATTTTGCTTA
TATCACTACTTATCACTAGACTATGTGTGCAATGAAGTCAGAGAAGGTGTTCGTAAAGTGACAATTTCACCACAAAAAGTATTAGTGTTAGAGAGGGACT
TATTTTCTGGGTAAAGTGAGCTTTTTAATGTAAACATTGGGGTAGAAACATGTTTCTTACTTCTTTCTAAAAACTTTCGGATGCTAGTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1233 bp411 aa791311+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGLFRRNKNNNKPVIRQILDLVPHWLFRSCTNTYKTDKGVHKYRTYDQFVALTFGQLNKCQSLNDISAGIGVSEIFISDLGLTQSPARSTMSDGNKKRDW
QVFESLYYRLLSHYKSVLKQHHNTHIIEEIKGKVVKLIDSSTISLCLAMFDWAEFRTAKGGIKLHTSWDYNLMIPDVVNITEAKVHDRYGLKQLIFPKDT
IIVEDRAYFDFELMLNRIKAENVFVTRIKSNTLYETIEELELADDVDQHILKDEIIQLTSGRAIETGISKHKLRLVHVYKEDENKVIAIITNQLDWEYNT
IAALYKKRWDIELFFKALKQNLQVKTFWGTSENAVKSQIYVALINYLLLELIKRTISKKAVAFSNLSEKVRFCLYHYLSLDYVCNEVREGVRKVTISPQK
VLVLERDLFSG

 

Blast result :
Comments
ISPto2 is 76% aa similar to ISCph2. ISPto2 was found by screening completely sequenced genomes for sequences homologous to the ISAva1 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-65-D(N3)-106-E(C1). The sequencing of the Psychroflexus torquis ATCC 700755 chromosome is currently in progress. Accession: NZ_AAPR00000000.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Bowman,J., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Unpublished