ISfinder Mirror Site

ISPepr3

Family IS4
Group IS4Sa

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Pelobacter propionicus	Pelobacter propionicus DSM 2379

DNA section

IS Length : 1337 bp

Ends

IR Length : 23/28

IRL : CAGCGTCGTCCGGTTAGGTCTTTGCATGGTTCTTTGAGAATGTAAAAAAG
IRR : CAGTAATTTCCGGTTAGGTCTTTGCATGTCGTCGTTTTTTCTTTAAAAGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGTAAGGTGT	ATGAGGAGT	ACATTACCGG	9

DNA sequence

CAGCGTCGTCCGGTTAGGTCTTTGCATGGTTCTTTGAGAATGTAAAAAAGTAACAAAATCAGCGTGATGCCACATTTTTTTTGCTTGACTTTTAGTAAAA
AATTGGCGGGCCACCATCGTAACTACTTAATATTACACGAGTTACGACATGCGACCTTTCAAGCGACTGAAACAACGACGAAAAGCACGTGCTTTCAAAT
TGCTTCTTACCCCGGTTTTTGAGAGATTCAAGTCCGATAAACAACTTGAATCCAGGGGGTACCGCCCGTTGCAAATGACCTTTGATGATCAGCTAAAGGC
CCTGATTTTCTATCACCTTGAAGAGTTTTCTTCCGGGAGCGAACTGCTGCAGGCTCTGGAACAGAATGACTTTGCCAAGGAGTGCGTTGCTCCCCCCAAG
GGGATCAAAAAGAGCGCCTTCTTCGAGGCGATCAATAACCGTGGCCTTGAGCAGCTAAGTGAGGTATTCGGACATCTGGTCAAGCAAGCAGGCAAAGTGC
TTCCGGCCGAATACGCTCACCTTGGCAACCTGGTATCCATAGATGGCTCTTTGATTGATGCTGTGCTGTCCATGGAATGGGCTGATTACCGAAGCGGCTC
AAAGAAGGCCAAGGCTCATGTCGGCTTCGATATCAACCGGGGCATTCCCAGGAAGATATACCTCAGCGACGGTAAAGAGGGTGAGCGCCCCTTTGTTGAC
AAGATCATCGACAAAGGCGAAACCGGCGTTATGGATCGCGGGTATCAGTCCCATGACCACTTTGACAAGTGGCAGGCCGCCGAGAAGTTCTTTGTCTGTC
GTATCAGGGAGAATACGATCAAGATAGTCATCAGAGAGAATGCCGTCAATCCTGACAGTATTATCTTCTATGACCGGATCGTAATGCTCGGCACCAAAGG
CGTAAACCAGACTGAGAAAGAACTGCGCCTTGTTGGTTACCGTGTTGACGGTAAAGATTACTGGATCGCTACCAACAGGTACGACCTGACCGCTGAACAA
GTCGCCGAAGTCTATAAGCTCCGCTGGAACATCGAGACCTTCTTTGGCTGGTGGAAACGCCATCTCAAGGTGTACCACCTGATCGCCAGGAGCAAGTACG
GGCTGATGGTCCAGCTTCTTGGCGGCCTTATAACCTATCTGCTTCTTGCTATTTACTGCCGGGAGCAGCACAACGAACCAGTCAGCATCACTCGGGTACG
GGAATTACGCAACCAAATCGCCAATGAGGCAGCAGAAGAACTAGAGCAAAAACGGATGCGAACACAGCAAAAATCCCACAAAAACAAACTTTTAAAGAAA
AAACGACGACATGCAAAGACCTAACCGGAAATTACTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1173 bp	391 aa	149	1321	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRPFKRLKQRRKARAFKLLLTPVFERFKSDKQLESRGYRPLQMTFDDQLKALIFYHLEEFSSGSELLQALEQNDFAKECVAPPKGIKKSAFFEAINNRGL
EQLSEVFGHLVKQAGKVLPAEYAHLGNLVSIDGSLIDAVLSMEWADYRSGSKKAKAHVGFDINRGIPRKIYLSDGKEGERPFVDKIIDKGETGVMDRGYQ
SHDHFDKWQAAEKFFVCRIRENTIKIVIRENAVNPDSIIFYDRIVMLGTKGVNQTEKELRLVGYRVDGKDYWIATNRYDLTAEQVAEVYKLRWNIETFFG
WWKRHLKVYHLIARSKYGLMVQLLGGLITYLLLAIYCREQHNEPVSITRVRELRNQIANEAAEELEQKRMRTQQKSHKNKLLKKKRRHAKT

Blast result :

Comments

ISPepr3 is 46% aa similar to ISDre1. ISPepr3 was found by screening completely sequenced genomes for sequences homologous to the ISPg4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-63-D(N3)-99-E(C1). The sequencing of the Pelobacter propionicus DSM 2379 chromosome is currently in progress. Accession: NZ_AAJH00000000.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2005) Unpublished