ISPca1
- Family IS4
- Group IS4Sa
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007498 | ND | Pelobacter carbinolicus | Pelobacter carbinolicus DSM 2380 |
DNA section
IS Length : 1251 bp
Ends
IR Length : 19
IRL : CACTGCTGTCCCATAGTTTGATCAATAAATAGAAGGCTCGGAACCCGTTA
IRR : CACTGCTGTCCCATAGTTTTAAATGAGCGACAACTGTTTGTCGCATAACA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTTTGTTTTG | CTAATGAGT | ATGAAGTCTT | 9 |
DNA sequence
CACTGCTGTCCCATAGTTTGATCAATAAATAGAAGGCTCGGAACCCGTTAGTGTTATAATGATTTCGCCAAAAAACACAACAACACCGAGGAGTTCCAAG
CCATGCCACATTGTAGCACCGTTCTTTCTCAAATAGTACGCATTTTCCCGAGACATGAATTTCAAGCTCTGGCGAAAAAGTATCACGCTGGACAAAAATT
CCGCTCATTTTCCCGCTGGACTCAGTTTGTTTCCTTGCTGACCGCTCAATTGACTGGTCGTGACAGCCTGCGAGATATCGTAGAGAACATGTCGGCTCAA
ACCAGCAAGCTTTATCACCTCGGGATCAAGCCGTTCAGTCGGTCAACTTTGGCCAGGACCAACGAAACGCAACCTCACCAGATGTACGAGGCTTTGTTTC
AACGTTTGCTGACCCGCTGCCAGGGCCTTGCCCCGACAAAACGCTTCAAATTCAAGGAGAAACTCTATTTACTTGATGCCTCCATGATTGACCTGACCTT
ATCGCTTTTTCCTTGGGCCAAGTACCGCAAGAGCAAGGGAGCCGCAAAGCTCCACATCGGATTGGATGCCGATGGTTACTTGCCTGCGTTTGTCGATATG
ACCGAAGGCAAAGAACATGAAATCAATAGGGCCAGAGAGCTTGAACTGCCCAAGGGCTCATACGTTGTTTTCGACCGTGGTTACACCGACTACACTTGGT
ATCAGGACCTCTGCGAAGAGGATATCCGCTTTGTTACTCGGCTCAAGCGCAACGCCGTGGCGATTTCCGGACCGAAGCGCAGAGGGCGTAGAAGTCCCGG
CGTGCTACGGGATCAACAGATTCAACTCAAGGGTGTCGCTGGAACCTTCCGCAAGGTTGTGTATCTGGATGCTGAAACCAATATCACCTACGAGTTTTTG
ACCAATGCGCTGGATATCCCGGCAGCAACGGTTGCCGAATTATACAAAGAGCGCTGGCAGATCGAGCTGTTCTTTAAGTGGATCAAACAAAACCTGCGCG
TGAAGAGCTTTCTGGGGACTTCTCGCAATGCTGTGATGACCCAACTCTGGATCGCTCTGTGTGCCTATCTGGTGCTGGCGTTTTTGAAGTTTCAATCGAA
ACTTGGGCACTCCATGCAGCAGATGTTGCGGTTATTACAGTTAAATTTGTTCGAGCGACGCGATTTTATGGAGCTGTTTGCGCCGCCCAGACCAAAAAGG
TTGTTATGCGACAAACAGTTGTCGCTCATTTAAAACTATGGGACAGCAGTG
CCATGCCACATTGTAGCACCGTTCTTTCTCAAATAGTACGCATTTTCCCGAGACATGAATTTCAAGCTCTGGCGAAAAAGTATCACGCTGGACAAAAATT
CCGCTCATTTTCCCGCTGGACTCAGTTTGTTTCCTTGCTGACCGCTCAATTGACTGGTCGTGACAGCCTGCGAGATATCGTAGAGAACATGTCGGCTCAA
ACCAGCAAGCTTTATCACCTCGGGATCAAGCCGTTCAGTCGGTCAACTTTGGCCAGGACCAACGAAACGCAACCTCACCAGATGTACGAGGCTTTGTTTC
AACGTTTGCTGACCCGCTGCCAGGGCCTTGCCCCGACAAAACGCTTCAAATTCAAGGAGAAACTCTATTTACTTGATGCCTCCATGATTGACCTGACCTT
ATCGCTTTTTCCTTGGGCCAAGTACCGCAAGAGCAAGGGAGCCGCAAAGCTCCACATCGGATTGGATGCCGATGGTTACTTGCCTGCGTTTGTCGATATG
ACCGAAGGCAAAGAACATGAAATCAATAGGGCCAGAGAGCTTGAACTGCCCAAGGGCTCATACGTTGTTTTCGACCGTGGTTACACCGACTACACTTGGT
ATCAGGACCTCTGCGAAGAGGATATCCGCTTTGTTACTCGGCTCAAGCGCAACGCCGTGGCGATTTCCGGACCGAAGCGCAGAGGGCGTAGAAGTCCCGG
CGTGCTACGGGATCAACAGATTCAACTCAAGGGTGTCGCTGGAACCTTCCGCAAGGTTGTGTATCTGGATGCTGAAACCAATATCACCTACGAGTTTTTG
ACCAATGCGCTGGATATCCCGGCAGCAACGGTTGCCGAATTATACAAAGAGCGCTGGCAGATCGAGCTGTTCTTTAAGTGGATCAAACAAAACCTGCGCG
TGAAGAGCTTTCTGGGGACTTCTCGCAATGCTGTGATGACCCAACTCTGGATCGCTCTGTGTGCCTATCTGGTGCTGGCGTTTTTGAAGTTTCAATCGAA
ACTTGGGCACTCCATGCAGCAGATGTTGCGGTTATTACAGTTAAATTTGTTCGAGCGACGCGATTTTATGGAGCTGTTTGCGCCGCCCAGACCAAAAAGG
TTGTTATGCGACAAACAGTTGTCGCTCATTTAAAACTATGGGACAGCAGTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1128 bp | 376 aa | 103 | 1230 | + | No |
Chemistry : DDE
ORF sequence :
MPHCSTVLSQIVRIFPRHEFQALAKKYHAGQKFRSFSRWTQFVSLLTAQLTGRDSLRDIVENMSAQTSKLYHLGIKPFSRSTLARTNETQPHQMYEALFQ
RLLTRCQGLAPTKRFKFKEKLYLLDASMIDLTLSLFPWAKYRKSKGAAKLHIGLDADGYLPAFVDMTEGKEHEINRARELELPKGSYVVFDRGYTDYTWY
QDLCEEDIRFVTRLKRNAVAISGPKRRGRRSPGVLRDQQIQLKGVAGTFRKVVYLDAETNITYEFLTNALDIPAATVAELYKERWQIELFFKWIKQNLRV
KSFLGTSRNAVMTQLWIALCAYLVLAFLKFQSKLGHSMQQMLRLLQLNLFERRDFMELFAPPRPKRLLCDKQLSLI
RLLTRCQGLAPTKRFKFKEKLYLLDASMIDLTLSLFPWAKYRKSKGAAKLHIGLDADGYLPAFVDMTEGKEHEINRARELELPKGSYVVFDRGYTDYTWY
QDLCEEDIRFVTRLKRNAVAISGPKRRGRRSPGVLRDQQIQLKGVAGTFRKVVYLDAETNITYEFLTNALDIPAATVAELYKERWQIELFFKWIKQNLRV
KSFLGTSRNAVMTQLWIALCAYLVLAFLKFQSKLGHSMQQMLRLLQLNLFERRDFMELFAPPRPKRLLCDKQLSLI
Blast result :
Comments
ISPca1 is 79% aa similar to ISGsu1. ISPca1 was found by screening completely sequenced genomes for sequences homologous to the ISAva1 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-65-D(N3)-96-E(C1). The copy number on the Pelobacter carbinolicus DSM 2380 chromosome is 1.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Chertkov,O., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Ivanova,N., DiDonato,R., Blunt-Harris,E., Lovley,D. and Richardson,P.(2006) Unpublished
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Chertkov,O., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Ivanova,N., DiDonato,R., Blunt-Harris,E., Lovley,D. and Richardson,P.(2006) Unpublished