ISGme2
- Family IS4
- Group IS4Sa
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007515 | ND | Geobacter metallireducens | Geobacter metallireducens GS-15 |
DNA section
IS Length : 1314 bp
Ends
IR Length : 15/17
IRL : TACTACTGTCCGGTAAACTTTGATTGTACAGCTGTAACTTGTTGAACTTT
IRR : CACTACTGTCCGGTTAATAGTTGAATAAGTTCAGTTGGTTGCGCTCAGGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATATTTTTAT | AGTGGAATA | CACTATTAAC | 9 |
| TTCGTTTGTT | GATCGGCAT | CCAATTTTGA | 9 |
| CCGAATCGTT | ATCCAAAAT | AGGAAATTCT | 9 |
| GATTAAATAT | AAATAAAAT | ATATAATATT | 9 |
| AAGGATTACT | ATACTGATT | ATGAAAGAAG | 9 |
| GTTATTTATT | AGTCGCCCG | TTTTTTTCTC | 9 |
| GCGATTAAAT | ATCTTTATA | CACATTTCTT | 9 |
| AAGTTTTCAG | ATATCGAAA | CGAAAAAATT | 9 |
| AGCACACAAC | GGGGACAAA | AGCAAAAAGC | 9 |
| CGGTATTTAT | GGTGATCAT | GGTTTTTGCT | 9 |
DNA sequence
TACTACTGTCCGGTAAACTTTGATTGTACAGCTGTAACTTGTTGAACTTTATTGCTCTTTGGCACAATTGTTCTTTTTTCGACATGAATTCGTTCTGAGT
GTAGCCTTCCTCCCTTTACAGGAGGGGAGCAATGCACACCGGTCCGACCGTTTTCAAACAACTTCTGCAGTTTCTGCCCCGGTACGAATTCAATCTCTGC
GTTCGCCGACACCGTGGCGAGTATCGGGAGAAGAAGTTCTCGACCTATGACCAGTTCCTCTGCCTGGCTTATGCCCAGATGGCTGGCCGTGAGAGCTTGC
GGGATATCGAGACCTGCCTGAACTCCCACCAGGAGAAGCTGTACCACATCGGCTTTCGTGGTGATGTCTCCCGCACGACCCTTGCCGACGCGAACGAGCG
CAGGGACTGGCGTATCTTCCAAGACTTCGGTCATGTACTGATCGGCATAGCTCAGCAGCTGTACCAGGCTGATGCCATCTCCGTTGAGCTTACGCAACCG
CTCTACGCCTTTGACTCGACCACTATCGACCTGTGCCTTACGCTGTTCCCATGGGCCGAGTTCCGCAAAACCAAGGCGGCGGTCAAGATGCATACGCTCA
TTGATCTGCGTGGTCCCATTCCGACCTGGGTCACTATTACCACTGGCAAGGTCCACGATGTCAGGATGCTGGACCATCTGCCGGTTGCAAAGGATGCCAT
TTACACGATGGACAGAGGGTATGTCGATTTTGCCCGGCTCCACTCCATCCACAAGCAGGGAGCATTCTTCGTAGTTCGGGCAAAGGACAACCTGAAATGC
CAGCGGTTATATTCCCATCCGAAGGACAAGGAATCAGGTGTACGGGCAGACCAGATTATCACCCTGGTGACGCAGAAGTCGAAAAAGGGGTATCCGGAGA
AACTGCGCCGGGTCAGCTATGTTGACAAAGAACGGAACAAGCGACTCGTATTTCTCACGAACAACTTCGAGATTCCGGCAGCGACGGTGGCTGCGATTTA
CAAGCAGCGCTGGCAGGTGGAGCTGTTTTTCAAATGGATCAAACAGCACCTGCGGATCAAGTCGTTCATCGGAACGTCAGTCAATGCCGTGAAGAGCCAG
ATATGGGTTGCCTTGTGCATCTATCTGCTGGTGGCGATCACGAAAAAGAAGTTGGGGGTTCCGTGTTCGCTCTACACTTTTCTACAGATTCTGGAGGTCA
ACTTGTTCGAGAAAAAGCCCATTTCATCGCTGGTTGCGGAGGCTCTCAAGCGAAATGCTGATTATCCTGAGCGCAACCAACTGAACTTATTCAACTATTA
ACCGGACAGTAGTG
GTAGCCTTCCTCCCTTTACAGGAGGGGAGCAATGCACACCGGTCCGACCGTTTTCAAACAACTTCTGCAGTTTCTGCCCCGGTACGAATTCAATCTCTGC
GTTCGCCGACACCGTGGCGAGTATCGGGAGAAGAAGTTCTCGACCTATGACCAGTTCCTCTGCCTGGCTTATGCCCAGATGGCTGGCCGTGAGAGCTTGC
GGGATATCGAGACCTGCCTGAACTCCCACCAGGAGAAGCTGTACCACATCGGCTTTCGTGGTGATGTCTCCCGCACGACCCTTGCCGACGCGAACGAGCG
CAGGGACTGGCGTATCTTCCAAGACTTCGGTCATGTACTGATCGGCATAGCTCAGCAGCTGTACCAGGCTGATGCCATCTCCGTTGAGCTTACGCAACCG
CTCTACGCCTTTGACTCGACCACTATCGACCTGTGCCTTACGCTGTTCCCATGGGCCGAGTTCCGCAAAACCAAGGCGGCGGTCAAGATGCATACGCTCA
TTGATCTGCGTGGTCCCATTCCGACCTGGGTCACTATTACCACTGGCAAGGTCCACGATGTCAGGATGCTGGACCATCTGCCGGTTGCAAAGGATGCCAT
TTACACGATGGACAGAGGGTATGTCGATTTTGCCCGGCTCCACTCCATCCACAAGCAGGGAGCATTCTTCGTAGTTCGGGCAAAGGACAACCTGAAATGC
CAGCGGTTATATTCCCATCCGAAGGACAAGGAATCAGGTGTACGGGCAGACCAGATTATCACCCTGGTGACGCAGAAGTCGAAAAAGGGGTATCCGGAGA
AACTGCGCCGGGTCAGCTATGTTGACAAAGAACGGAACAAGCGACTCGTATTTCTCACGAACAACTTCGAGATTCCGGCAGCGACGGTGGCTGCGATTTA
CAAGCAGCGCTGGCAGGTGGAGCTGTTTTTCAAATGGATCAAACAGCACCTGCGGATCAAGTCGTTCATCGGAACGTCAGTCAATGCCGTGAAGAGCCAG
ATATGGGTTGCCTTGTGCATCTATCTGCTGGTGGCGATCACGAAAAAGAAGTTGGGGGTTCCGTGTTCGCTCTACACTTTTCTACAGATTCTGGAGGTCA
ACTTGTTCGAGAAAAAGCCCATTTCATCGCTGGTTGCGGAGGCTCTCAAGCGAAATGCTGATTATCCTGAGCGCAACCAACTGAACTTATTCAACTATTA
ACCGGACAGTAGTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1167 bp | 389 aa | 114 | 1280 | + | No |
Chemistry : DDE
ORF sequence :
MHTGPTVFKQLLQFLPRYEFNLCVRRHRGEYREKKFSTYDQFLCLAYAQMAGRESLRDIETCLNSHQEKLYHIGFRGDVSRTTLADANERRDWRIFQDFG
HVLIGIAQQLYQADAISVELTQPLYAFDSTTIDLCLTLFPWAEFRKTKAAVKMHTLIDLRGPIPTWVTITTGKVHDVRMLDHLPVAKDAIYTMDRGYVDF
ARLHSIHKQGAFFVVRAKDNLKCQRLYSHPKDKESGVRADQIITLVTQKSKKGYPEKLRRVSYVDKERNKRLVFLTNNFEIPAATVAAIYKQRWQVELFF
KWIKQHLRIKSFIGTSVNAVKSQIWVALCIYLLVAITKKKLGVPCSLYTFLQILEVNLFEKKPISSLVAEALKRNADYPERNQLNLFNY
HVLIGIAQQLYQADAISVELTQPLYAFDSTTIDLCLTLFPWAEFRKTKAAVKMHTLIDLRGPIPTWVTITTGKVHDVRMLDHLPVAKDAIYTMDRGYVDF
ARLHSIHKQGAFFVVRAKDNLKCQRLYSHPKDKESGVRADQIITLVTQKSKKGYPEKLRRVSYVDKERNKRLVFLTNNFEIPAATVAAIYKQRWQVELFF
KWIKQHLRIKSFIGTSVNAVKSQIWVALCIYLLVAITKKKLGVPCSLYTFLQILEVNLFEKKPISSLVAEALKRNADYPERNQLNLFNY
Blast result :
Comments
ISGme2 is 59% aa similar to ISGsu1.
ISGme2 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-65-D(N3)-102-E(C1). The copy number in Geobacter metallireducens GS-15 is 10.
ISGme2 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-65-D(N3)-102-E(C1). The copy number in Geobacter metallireducens GS-15 is 10.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., lavina,T., Hammon,N., Israni,S., Pitluck,S., Di Bartolo,G., Chain,P., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Ivanova,N. and Richardson,P.
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18