ISfinder Mirror Site

ISFac10

Family IS4
Group IS4Sa

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Ferroplasma acidarmanus	Ferroplasma acidarmanus Fer1

DNA section

IS Length : 1354 bp

Ends

IR Length : 17/19

IRL : CATACCAGTTTCCTATATTTAACCATTATGCATGACCATGTCATATTTAT
IRR : CATACAGATTTCCAATATTTATATCTGATCATTTGCAATTTCATAAAGAT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TATTAATTAT		TCATAATAAT	0

DNA sequence

CATACCAGTTTCCTATATTTAACCATTATGCATGACCATGTCATATTTATTCTATGATTTAATTTTTAATTATGGATGAGAATGTCAATAAAATCCTTAA
AAAACACTGCAATAAAATATCTAAAGAATCAAAGGTTGAAGGAATAGGCATAGACAGCTATTCCAGGAAATTTAACCTATCAGCACATCTATCCATTTTA
GCAACTGGAATAATTAAAAAGAATGACCTTACAGATATAGCATATAACAATGGAATAAGCAAATCACAGCTGTCAAAGCTTAACAATAAAAGGCCATACA
GTATATTTGAAAAGGTATTTTACAGTATTCTGAGGCCCTTTATAAAGGCACACAGGTATGATATATACCATGATTATATTGACAGATTATACAGTGTTCT
GGCAATAGATTCTACATTTATAGAAACAATGGTTAAGGGTTCAGGCATCTATCAGAGGGGGGAAAGGAGAAATGGAATAAAAATACATACTGCAGCCATT
GCAAGCCCCTATCCTTTGCCATTGAAGGCAATAATTACACCTGCAAATGTACATGATTCTAAAGTATTTGATGATCTGCTGGAATATATCAATGAATACA
TATCTGGCAATACAGTATTAACATTTGATTTAGGGTACTATAATCTTGGCAGGTTCATGGAGTTAAAGGAAAAAGGTATAAATTTTGTTTCAAGGATAAA
GAAGAATGCTGATTATACTGTAATAAAGGAGGAAACATTCAATTCTAAGATTGTAAGGTTTAGAAATGGATTGGAATTAAGATTGGTATCACTGGATATT
AATAATAGGAAAAGGGAATATATAACAGATATACTGGACCTTCCTGAAATATACATTTATTATATCTACATGAGAAGATGGGTAATAGAGAAAATATTTG
AAAATATGAAAAGGATTTTAAAAATAACACATTTAATATCAAGGGATTTAAATGGAATTATAAACCAGGTATTTGCAACTCTTATTTCCTATATTGTACT
GTTGATATTGCAATCATCAATGAATATATACCATACTCCTGCGGAAATAGTAAGGTCAATAAGGAACAGTAAAGAGCTTGATATTGGCCATGATTACATG
AGCAATTTCAATAAAATATAATAGCAATAAATTAAATCGGGCTTACTTAATAGTAAGCCCTTACCCCTTCCATCAGGCAATATTATGATCTTAATTTTTT
ATAATAATTTTTCCATTGGATTTACAACCAGCAATCAGAGAATTTTAATTATAATTCCATTGCTCGACAATAATTGTCATAGCAACACTGTGTAAATTGA
TGTTATCTTTATGAAATTGCAAATGATCAGATATAAATATTGGAAATCTGTATG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1047 bp	349 aa	72	1118	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDENVNKILKKHCNKISKESKVEGIGIDSYSRKFNLSAHLSILATGIIKKNDLTDIAYNNGISKSQLSKLNNKRPYSIFEKVFYSILRPFIKAHRYDIYH
DYIDRLYSVLAIDSTFIETMVKGSGIYQRGERRNGIKIHTAAIASPYPLPLKAIITPANVHDSKVFDDLLEYINEYISGNTVLTFDLGYYNLGRFMELKE
KGINFVSRIKKNADYTVIKEETFNSKIVRFRNGLELRLVSLDINNRKREYITDILDLPEIYIYYIYMRRWVIEKIFENMKRILKITHLISRDLNGIINQV
FATLISYIVLLILQSSMNIYHTPAEIVRSIRNSKELDIGHDYMSNFNKI

Blast result :

Comments

ISFac10 is 44% aa similar to IS4Bsu1. ISFac10 was found by screening completely sequenced genomes for sequences homologous to the ISAva1 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-72-D(N3)-86-E(C1). The sequencing of the Ferroplasma acidarmanus Fer1 chromosome is currently in progress. Accession number: NZ_AABC00000000.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2005) Unpublished.