ISDha3
- Family IS4
- Group IS4Sa
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007907 | ND | Desulfitobacterium hafniense | Desulfitobacterium hafniense DCB-2 Desulfitobacterium hafniense Y51 |
DNA section
IS Length : 1593 bp
Ends
IR Length : 18/21
IRL : CAGTAGCGTTGCATAAAGATAGCAACGAAAAAGTCAAGGTTTAAATTTGC
IRR : CAGTAGCGTTGCATTGAAATAAAAAGCTTATGTCAATAGAAAAACCCTCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCAAAATCT | ATATCTTTA | TCTTTTGTTC | 9 |
DNA sequence
CAGTAGCGTTGCATAAAGATAGCAACGAAAAAGTCAAGGTTTAAATTTGCTCATATTTAAGCAAATTTCCTCGAAATCACCGGTTATTCCTAATTATTTC
TGTAAACATCATGAAAAAATCCTTATAATAAGAGATAGGGTAGTCCTTGCCCAAATCTCAAATTATAAGGAGATAACAATGCAGGACAAGGATACTACGC
AATCCACATTTATGCAAGCCTTTCAGCCGTTTTTTTCGAAAGATCTATGGGAAACTATTCACCGGGAGGTCCCTGGCCTTGACCTTCGGTCTCAAAAACT
AACAACCAATCAACTCACTCTCCTCATTAGCCATGCTCAACTTCAAGAATACAGAGCTTTGCGAAAGATCAGTACAAGTGTTCACCATGACAGTTTAGGT
CAAGCTATCGGACTCGAAAGCATTAGTCACAGCCAGATTTCCCGAAGGTTAAAGACCTTACCCACTCAAGTTCCGGAAATGCTTTTTAAGGGCACTTTAC
ATAACGTAGCTCAAAAGCAAGGGTATGGGAAGATTCGGCAGCAACTCGGAAAATTGTACATGATTGATGCATCCACCATGAGCCTATGCCTTTCCCGCTA
CCCCTGGGCAGTCTTTAGAAAGAGCAAAGCCGGTGTCAAAATGCATCTGCGTTTAAGCTTTGATGCAATGGCTGTCCCGGATGAAGTGCTCGTTACCCCG
GCCAAGACCGCCGACCGCAAGAAACTGGATGAGCTCATTGTTCAAGACCAAGAGGCTTTGAATATCTTTGATCGGGGTTATGTGGATTATAAGCTCTTTG
ATGATTACTGTGAAAAGGGAATACGCTTTGTGACTCGTCTCAAAAACAACGCGGTGATAGAGTTTACTGGAGTAGAACGTCCAGTCAAAGAAGATGGGCT
CATCGAAGAGGACGTGGATGTCATCTTAGGAGCAGGTTCTCGAAAAATGAAGCATACCCTTCGGGAAGTGACGATAGATGATAACGTCCATGAACCCTTC
ACCATCCTGACGAATGATTTTAACCTAAGTGCCGAAGAACTCGGTGAGATCTATCGCTATCGCTGGCAGATTGAGTTATTTTTTAAATGGCTCAAGCAGC
ATGCTCAAATTAAACACTTTTACGGCACGAGTGAGACCGCAGTCATCAACCAAATCCTCCTAGCCCTGATGATGTATTGCTTATTAGTTCTGCTCAAGCT
TGAGGCGGGCTATCCAAGAGATTTACTCACTTTGCAGCGACTGCTGATCGCCTGTCTGTTTGAGAGGTATGAAGAGTTTTTGGAGAAGCTGCGACGACGA
AGGAGAAAGGGCTCCAAACGAATCCGTTATGAGGAGATCTATAGAATGACGGAGCATTCTATCTTGAAAGAAGAAGAGACCCAATGGCTCGATGATTTAA
CCTATGACCCAGTGATACTGTAAAGCATATGTGTAAAAATGTGGAATAGGACTGCCTTTTAGGTGAATGTTCCTTTTGGCAGAAGAGAGAGAAAGCAAAT
GGTTGTATACACCATATAAGGGATTTATGAACAATTGAAATTATGAGGGTTTTTCTATTGACATAAGCTTTTTATTTCAATGCAACGCTACTG
TGTAAACATCATGAAAAAATCCTTATAATAAGAGATAGGGTAGTCCTTGCCCAAATCTCAAATTATAAGGAGATAACAATGCAGGACAAGGATACTACGC
AATCCACATTTATGCAAGCCTTTCAGCCGTTTTTTTCGAAAGATCTATGGGAAACTATTCACCGGGAGGTCCCTGGCCTTGACCTTCGGTCTCAAAAACT
AACAACCAATCAACTCACTCTCCTCATTAGCCATGCTCAACTTCAAGAATACAGAGCTTTGCGAAAGATCAGTACAAGTGTTCACCATGACAGTTTAGGT
CAAGCTATCGGACTCGAAAGCATTAGTCACAGCCAGATTTCCCGAAGGTTAAAGACCTTACCCACTCAAGTTCCGGAAATGCTTTTTAAGGGCACTTTAC
ATAACGTAGCTCAAAAGCAAGGGTATGGGAAGATTCGGCAGCAACTCGGAAAATTGTACATGATTGATGCATCCACCATGAGCCTATGCCTTTCCCGCTA
CCCCTGGGCAGTCTTTAGAAAGAGCAAAGCCGGTGTCAAAATGCATCTGCGTTTAAGCTTTGATGCAATGGCTGTCCCGGATGAAGTGCTCGTTACCCCG
GCCAAGACCGCCGACCGCAAGAAACTGGATGAGCTCATTGTTCAAGACCAAGAGGCTTTGAATATCTTTGATCGGGGTTATGTGGATTATAAGCTCTTTG
ATGATTACTGTGAAAAGGGAATACGCTTTGTGACTCGTCTCAAAAACAACGCGGTGATAGAGTTTACTGGAGTAGAACGTCCAGTCAAAGAAGATGGGCT
CATCGAAGAGGACGTGGATGTCATCTTAGGAGCAGGTTCTCGAAAAATGAAGCATACCCTTCGGGAAGTGACGATAGATGATAACGTCCATGAACCCTTC
ACCATCCTGACGAATGATTTTAACCTAAGTGCCGAAGAACTCGGTGAGATCTATCGCTATCGCTGGCAGATTGAGTTATTTTTTAAATGGCTCAAGCAGC
ATGCTCAAATTAAACACTTTTACGGCACGAGTGAGACCGCAGTCATCAACCAAATCCTCCTAGCCCTGATGATGTATTGCTTATTAGTTCTGCTCAAGCT
TGAGGCGGGCTATCCAAGAGATTTACTCACTTTGCAGCGACTGCTGATCGCCTGTCTGTTTGAGAGGTATGAAGAGTTTTTGGAGAAGCTGCGACGACGA
AGGAGAAAGGGCTCCAAACGAATCCGTTATGAGGAGATCTATAGAATGACGGAGCATTCTATCTTGAAAGAAGAAGAGACCCAATGGCTCGATGATTTAA
CCTATGACCCAGTGATACTGTAAAGCATATGTGTAAAAATGTGGAATAGGACTGCCTTTTAGGTGAATGTTCCTTTTGGCAGAAGAGAGAGAAAGCAAAT
GGTTGTATACACCATATAAGGGATTTATGAACAATTGAAATTATGAGGGTTTTTCTATTGACATAAGCTTTTTATTTCAATGCAACGCTACTG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1242 bp | 414 aa | 179 | 1420 | + | No |
Chemistry : DDE
ORF sequence :
MQDKDTTQSTFMQAFQPFFSKDLWETIHREVPGLDLRSQKLTTNQLTLLISHAQLQEYRALRKISTSVHHDSLGQAIGLESISHSQISRRLKTLPTQVPE
MLFKGTLHNVAQKQGYGKIRQQLGKLYMIDASTMSLCLSRYPWAVFRKSKAGVKMHLRLSFDAMAVPDEVLVTPAKTADRKKLDELIVQDQEALNIFDRG
YVDYKLFDDYCEKGIRFVTRLKNNAVIEFTGVERPVKEDGLIEEDVDVILGAGSRKMKHTLREVTIDDNVHEPFTILTNDFNLSAEELGEIYRYRWQIEL
FFKWLKQHAQIKHFYGTSETAVINQILLALMMYCLLVLLKLEAGYPRDLLTLQRLLIACLFERYEEFLEKLRRRRRKGSKRIRYEEIYRMTEHSILKEEE
TQWLDDLTYDPVIL
MLFKGTLHNVAQKQGYGKIRQQLGKLYMIDASTMSLCLSRYPWAVFRKSKAGVKMHLRLSFDAMAVPDEVLVTPAKTADRKKLDELIVQDQEALNIFDRG
YVDYKLFDDYCEKGIRFVTRLKNNAVIEFTGVERPVKEDGLIEEDVDVILGAGSRKMKHTLREVTIDDNVHEPFTILTNDFNLSAEELGEIYRYRWQIEL
FFKWLKQHAQIKHFYGTSETAVINQILLALMMYCLLVLLKLEAGYPRDLLTLQRLLIACLFERYEEFLEKLRRRRRKGSKRIRYEEIYRMTEHSILKEEE
TQWLDDLTYDPVIL
Blast result :
Comments
ISDha3 is 59% aa similar to IS4Bsu1.
ISDha3 was found by screening completely sequenced genomes for sequences homologous to the ISPpu8 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-67-D(N3)-100-E(C1).
Genome in progress : Accession number : NZ_AAAW04000002.1
ISDha3 was found by screening completely sequenced genomes for sequences homologous to the ISPpu8 transposase using BLASTP. Multiple alignments revealed a conserved DDE motif : D(N2)-67-D(N3)-100-E(C1).
Genome in progress : Accession number : NZ_AAAW04000002.1
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P. and Richardson,P.(2006)Unpublished
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P. and Richardson,P.(2006)Unpublished