ISNpu8

  • Family IS4
  • Group ISH8
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Nostoc punctiforme
Nostoc punctiforme PCC 73102
DNA section
IS Length : 1412 bp

Ends


IR Length : 18

IRL : CATTGGTGTCAACTTAAGCAAAAAGTAAGCAAAAGATGTAATCTAAGAAC
IRR : CATTGGTGTCAACTTAAGAAGATTTTGCCCAAATTTGTTGGGTGTAAGCG

Insertion site


Left flankDirect repeatRight flankDR Length
ACTAGGGGCGCACATCTGTGTGCCCCTAC9

DNA sequence

CATTGGTGTCAACTTAAGCAAAAAGTAAGCAAAAGATGTAATCTAAGAACATTACTAATGATGTTCTTAGAGATACATCATGGCAAGAAAACGCCCAGCG
CGAACAGGAAACCCAGACCTGCGGCAACAAAAACAAGTACCGATGCCGCCAATAGAGGAAATTGAACAAAAAATCTACTCATTACTATCTCCGCTCAACT
TTAAACCCTTGAAATTGTACGAAACGGAAGAAAAGAAACCATTCCGAGACAGAATACTGACCTTATCGGTAATGATGGCGCTCGTTGTGAGTTTAGTATA
TCGTCAGATTCCTGGATTAAGAGAAGTACAAAGAGTCCTATGCGAAGAAGGATTATTGTGGGCAGGTCGGATTGAAGTGAGTGCCCAAGCAGTTTCAAAA
CGATTGAGAACACTACCAATAGAATTATTTGCACAAATTTTCGAGCAAGTAATGGAAAGAATGAATGTGCAGCCACAAAATCAGGCAGTACCAGAGAATT
GGCAGCCAGTATGCGCCAAATTTACAGCCATCTGGATTGCTGACGGTTCAACCCTAGAAGCACTAAGACGCAAACTCAAAGTACTACAAGAACAAGAAAA
AACACTTGCTGGCAAAATTATGATGGTGGTAGAAGCATTTAGCCATCACCCAGTCACAACCTGGTATACGCAAAACAGCAAAGCCAATGATAAAACTTGG
TGCGAACAATTACTTGAACGCCTACCAATTGGTGGATTGCTGATTTTTGATTTAGGATTTTTTAAGTTTCCTTGGTTTGATGCGTTCACAGAAGCTGATA
AGTTTTTTTTGACAAGACTGCGAGAAAAAACTTCTTACAAGGTGATACGTTGTTTGACCAATGCCTCATTCTACCGTAATGAAATTATATCTATGGGGGA
ATATCGCTCTAATCCTTGCCAGCACCAAGTACGTTTAGTTTCTGTTTTGTGGGGTTCAACTTGGTACTATTACCTGACAAATGTACTCGACCCACAAATG
TTATCTGCTCATCTTGTCTGTGAATTAGGGAGCAGGCGCTGGCGCGTTGAAGATGCTTTTCTGCTCACAAAAAGACTTTTAGGTTTAGCTTATATTTGGG
TTGGGGACAACAATGGCGTACAAATCCAGATTTTTGCCACTTGGATTTTTTATGCTGTTCTTAACCAATTATGTATCGATATAGCGATCGCCCTCAACCA
ACCTTTAGACCGAATTTCTACAGAAATGGTATTTCGTGCTTTATACCATTTTTCACAAGCTGTTCTCCGTGGTGATGCACATGAGGCTGTTCCTTATCTT
GTGGAACGTCAAAAGCTGTTTGGATTAGTTAAAGCAAGGCGTAAGCGTCATCGAGAGATTGACGCTTACACCCAACAAATTTGGGCAAAATCTTCTTAAG
TTGACACCAATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1317 bp439 aa801396+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARKRPARTGNPDLRQQKQVPMPPIEEIEQKIYSLLSPLNFKPLKLYETEEKKPFRDRILTLSVMMALVVSLVYRQIPGLREVQRVLCEEGLLWAGRIEV
SAQAVSKRLRTLPIELFAQIFEQVMERMNVQPQNQAVPENWQPVCAKFTAIWIADGSTLEALRRKLKVLQEQEKTLAGKIMMVVEAFSHHPVTTWYTQNS
KANDKTWCEQLLERLPIGGLLIFDLGFFKFPWFDAFTEADKFFLTRLREKTSYKVIRCLTNASFYRNEIISMGEYRSNPCQHQVRLVSVLWGSTWYYYLT
NVLDPQMLSAHLVCELGSRRWRVEDAFLLTKRLLGLAYIWVGDNNGVQIQIFATWIFYAVLNQLCIDIAIALNQPLDRISTEMVFRALYHFSQAVLRGDA
HEAVPYLVERQKLFGLVKARRKRHREIDAYTQQIWAKSS

 

Blast result :
Comments
ISNpu8 is 57% aa similar to ISAva1. ISNpu8 was found by screening completely sequenced genomes for sequences homologous to the ISAva1 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-68-D(N3)-99-E(C1). The sequencing of the Nostoc punctiforme PCC 73102 chromosome is currently in progress. Accession: NZ_AAAY00000000.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] NCBI Microbial Genomes Annotation Project (2004) Unpublished