ISfinder Mirror Site

ISArch11

Family IS4
Group ISH8

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
AY714815	ND	uncultured archaeon	uncultured archaeon GZfos11A10

DNA section

IS Length : 1410 bp

Ends

IR Length : 17/18

IRL : CATGCGTGTTCGGATAAGAGATTGACATTCTAACTATTAATATTTTTTAT
IRR : CATGCGTGTTCGGTTAAGTAACCCATCGCTCCATCAACCTCTCCCGCTTA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AACGTCATGT	AAATATAGTT	AGTTGTGATA	10

DNA sequence

CATGCGTGTTCGGATAAGAGATTGACATTCTAACTATTAATATTTTTTATATAGCCTAAAAGCGATTCTTTTGCCTGGTGAGCCAATGTATGATTTCAGA
TAAGGAAAATAGTGTAGATCCTACGGTACAGACCATTGTTGAGATGTTTCCAGAGGATTTTTTGAGAAACACTGCAAGAGAAACAGGGGTTGTTAAACGG
GAGCGTAAAATTGACGTAGTCATCCTTTTTTGGGTAACAACGCTCGGGTTTGGGGTACGTTTTTTGAGTACTATCAGAGGGCTAAAACGGAAATACGAGG
AAAAAGCGAAGACCACGTTGAGCATAAGCAGTTTCCACGACCGATTCACGCCTGAGATGGCCGAGTTTCTCAGAAAGTGTGTGCTTCATGCGATTGCGTT
TCAAGCACAGCAAACCGGTCGTGTTTTGGATGACAAATTGAAGCGTTTTAAGGATTTGGTTATCCAAGACAGCACGATTATCCGGCTGCATGAATCTCTT
GCGAAGATATGGCCTGCTGCAAGGACAAAGAAAATCGCTGCTGGCGTTAAAGTCTCATGTATCGTTAGTGCTGTTGCCGATAGCCCTAAATCGGTGAGGA
TATACCCGGAAAGGACTTCAGAGGCTAAAATACTTCGATTGGGACCCTGGCTCAGGGACCGTATTCTGCTCATCGACCTTGGATACTTCAAATACCTATT
CTTTGACAGAATTGACGGTTATGGCGGCTATTTCGTCAGCAGGCTGAAAGGAAACGCCAATCCATTGATTGTTAGAGTAAATCGGAAATGTCGAGGAAAC
TCGGTAGATGTTGTGGGAAAGAAATTGAGAGATGTTCTTCCTCGCTTGAAGCGGGAGATACTTGATGTTGAAGTGGAGGTAGAATTCAAGAGGAGAAAGT
ATAAGGGAAAACAAAGCACGGTGAAGAGAAGATTCAGAATGGTATGTGCTTTCAATTCTGACTCAGGGAAGTATCACTCGTATTTGACTAATATTCGCGT
TGATATCTTATCCGCCGAGGAGATCGCACTCCTGTACGGAGCGAGATGGGAAATAGAACTGATATTCAAGGAGTTAAAAAGTCACTATCGTATGGATCAG
ATACCAAGTGCAAACCCAAACATTGTGAAATGCTTGATTTGGATTGCTATTCTGACGTTAATGTGCTCACGCAGAATACTACGGTTGATTCGGAATGCGA
ATCCTGAAAACGCTAATCGATACACGCCTTTACGCTGGGCAAAGGTGTTCTCCGAACAAGCAGACCGATTGTTAACCGAAGTGCTCGAATGCATGGGGCT
GAAACTTGATATGCTTACCTTATACGATATTTATCTCGGTCAAGGATGCGATCCTAATGTTAAGCGGGAGAGGTTGATGGAGCGATGGGTTACTTAACCG
AACACGCATG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1305 bp	435 aa	90	1394	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MISDKENSVDPTVQTIVEMFPEDFLRNTARETGVVKRERKIDVVILFWVTTLGFGVRFLSTIRGLKRKYEEKAKTTLSISSFHDRFTPEMAEFLRKCVLH
AIAFQAQQTGRVLDDKLKRFKDLVIQDSTIIRLHESLAKIWPAARTKKIAAGVKVSCIVSAVADSPKSVRIYPERTSEAKILRLGPWLRDRILLIDLGYF
KYLFFDRIDGYGGYFVSRLKGNANPLIVRVNRKCRGNSVDVVGKKLRDVLPRLKREILDVEVEVEFKRRKYKGKQSTVKRRFRMVCAFNSDSGKYHSYLT
NIRVDILSAEEIALLYGARWEIELIFKELKSHYRMDQIPSANPNIVKCLIWIAILTLMCSRRILRLIRNANPENANRYTPLRWAKVFSEQADRLLTEVLE
CMGLKLDMLTLYDIYLGQGCDPNVKRERLMERWVT

Blast result :

Comments

ISArch11 is 70% aa similar to ISMba6. ISArch11 was found by screening completely sequenced genomes for sequences homologous to the ISPa12 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-68-D(N3)-126-E(C1). The sequencing of the Uncultured archaeon GZfos11A10 chromosome is currently in progress. Accession: AY714815.

References

1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Hallam,S.J., Putnam,N., Preston,C.M., Detter,J.C., Rokhsar,D., Richardson,P.M. and DeLong,E.F.(2004) Science 305 (5689), 1457-1462