ISCku3

  • Family IS4
  • Group IS231
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CT573073 ND Candidatus Kuenenia
Candidatus Kuenenia stuttgartiensis
DNA section
IS Length : 1384 bp

Ends


IR Length : 21

IRL : CATAAGCGCTAACTTGTTTTATTGACAAATTTTATTCATCATGGTATTTT
IRR : CATAAGCGCTAACTTGTTTTAGGTATGCTCGAAGTATTTTGATATCTGTG

Insertion site


Left flankDirect repeatRight flankDR Length
GCATCCCACCCCCCTTGCCCCCCACTGGGA10
GGAAGGCCAGGGGTAGGTTAACGGCATAATG11
ATTAGCATCCACCCCTTGCCCACCCCCACTG11
GGGATGATACGCCTTCTGCCCCTTCTTGTGC11
CCGCTGGCGGGGGTGCAGGGGGTGGACTGGA11
AAAGGGATTGGGGATTTGCCCCTGAGTATGT11
GAAAACATGGGGGCTGNCCCCAGCCCCCCTT11
CCGCTGGCGGGGGTGCAGGGGGTGGACCGGA11
TCCAGTCCACCCCCTGCACCCCCGCCAGCGG11
TCAGGTTGCAGGGCACCGCCCCCGCAGCTCC11

DNA sequence

CATAAGCGCTAACTTGTTTTATTGACAAATTTTATTCATCATGGTATTTTTATGTTAATTATCCCGCTAATCACTCTATAGGAGGATAAAGCCATGATGA
GAGAAGATTGGGATCTTCTAAGAACTTTCTTCCCAAACGATTGGAAAAGTTTAGCCGTTGATACAAATGCTTTAAAAGGCTTGCGCAAGGATAAATCTGA
AGAAAAGCTTCTTCGAACATTATTAATTCATTTAGGATGTGGCTATTCATTGCGTGAAACAGTAGTTCTAGCCAAGCGTGCTAACTTAGCAGATTTATCC
GATGTTGCCTTATTAAAGCGATTAAAAAAGAGCAAAGAATGGCTATATAAATTATGTTTATCTTTATTCCGTGAGCGTGGCCTCCAAATTAATAAACGGA
ATAATTTTCATCTTCGCTTATTTGATGCAACAACAGTAAAGGAACCTGGGAAAACAGGAAGTCTTTGGCGCATTCATTATAGTATTGAGGTTCCTTCATT
ATCTTGCGATTTCTTTAAACTTACGGGAACTGAAGGAGAAGGCACAGGAGAATCTTTTCGGCAGTTTCCGATGAAAAAAGATGATTATATTATAGCTGAC
AGAGGTTACTGTACTGGCCAAGGAATTCATCATGCAACAAGGAAAGGCGCTTATCTTAGCGTTAGAGTTAATTCGCAATCTCTACGGATATTCGGCGAAG
AAAAGAAACCCTTTCCTTTATTGAAAGAAATCCAATATTTAAAAAGACCCCTTGCTATAAAATCATGGAACGTTTTTATTCCAAACGTTGATAATACTGA
ATATGTCAAAGGTCGTCTTTGTATAATACGCAAAACAGAAGAAGCCATTAAAATAGCTCATAAAAAACTTAAAAGACATGCAAGCAAAAAGGGCATTGAA
CTAAAACCGGAGACCCTTATTTATGCCAAGTACGTAATAGTATTCACAACGTTTCCTGAAAATCAATTTACCGCTTTTGATATCTTAGAATGGTATCGAG
TTCGATGGCAAATTGAACTGGTCTTTAAAAGATTTAAACAAATAGCACAATTTGGACACTTACCTAAATACGATGATGATAGCTCAAAAGCTTGGCTTTA
TGGCAAACTATTCGTTGCTCTTTTGACAGAAAAACTAATAGATTTTGCTACGTCTTTTTCCCCCTGGGGATACTTCATTGTCAAGCAAGAAGACTAAAAG
CAAATGGCGTGAATTTGCTTTTATGCTTAATCAAGTAAAACGGGCTATAGAACCAGCGTTATCATTACAGGAAGTTCTTAAGTGCTGGAATGACATTGCT
TGTTCTTTAGCAGAAAATACAAGAATCCGAAAAACACAGATATCAAAATACTTCGAGCATACCTAAAACAAGTTAGCGCTTATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1101 bp367 aa941194+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MMREDWDLLRTFFPNDWKSLAVDTNALKGLRKDKSEEKLLRTLLIHLGCGYSLRETVVLAKRANLADLSDVALLKRLKKSKEWLYKLCLSLFRERGLQIN
KRNNFHLRLFDATTVKEPGKTGSLWRIHYSIEVPSLSCDFFKLTGTEGEGTGESFRQFPMKKDDYIIADRGYCTGQGIHHATRKGAYLSVRVNSQSLRIF
GEEKKPFPLLKEIQYLKRPLAIKSWNVFIPNVDNTEYVKGRLCIIRKTEEAIKIAHKKLKRHASKKGIELKPETLIYAKYVIVFTTFPENQFTAFDILEW
YRVRWQIELVFKRFKQIAQFGHLPKYDDDSSKAWLYGKLFVALLTEKLIDFATSFSPWGYFIVKQED

 

Blast result :
Comments
ISCku3 is 46% aa similar to IS186B. ISCku3 was found by screening completely sequenced genomes for sequences homologous to the ISPa12 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-57-D(N3)-138-E(C1). The copy number on the Blastopirellula marina DSM 3645 chromosome is 10. The present IS is located on chromosome fragment 3. Some copies lie on chromosome fragment 4 (Accession: CT573072) or 5 (CT573071).
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18
2] Strous,M., Pelletier,E., Mangenot,S., Rattei,T., Lehner,A., Taylor,M.W., Horn,M., Daims,H., Bartol-Mavel,D., Wincker,P., Barbe,V., Fonknechten,N., Vallenet,D., Segurens,B., Schenowitz-Truong,C., Medigue,C., Collingro,A., Snel,B., Dutilh,B.E., Op den Camp,H.J., van der Drift,C., Cirpus,I., van de Pas-Schoonen,K.T., Harhangi,H.R., van Niftrik,L., Schmid,M., Keltjens,J., van de Vossenberg,J., Kartal,B., Meier,H., Frishman,D., Huynen,M.A., Mewes,H.W., Weissenbach,J., Jetten,M.S., Wagner,M. and Le Paslier,D.(2006)Nature 440 (7085), 790-794