ISBce9
- Family IS4
- Group IS231
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_007103 | ND | Bacillus cereus | Bacillus cereus E33L |
DNA section
IS Length : 1597 bp
Ends
IR Length : 17
IRL : CATCCATCAACTTAAGGTTTTAGACTATTTTTGAAGTTAAAAGCACGTTA
IRR : CATCCATCAACTTAAGGGATATTTTTAAACAAAATGTGGTTTTTTTATAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCCCACCACA | TGTGACGATA | 0 |
DNA sequence
CATCCATCAACTTAAGGTTTTAGACTATTTTTGAAGTTAAAAGCACGTTACTATTTTCTTATGTGAATGAGAAAGGGTGACGTGCTTTTTATGAATATGA
ATCAAAAACAAGAATTATCTTTATTCGCTGAAGAGTTATATCGATATATGTCTCCCGCTACACTTAATCAATTAGCTATAGAAGCAGGCGGGATGAAACG
AAAACGTAAGTGCCACGGGCATCATTTTTTATCTTTGTGTGTATGGTTAAATCAACAAGTCGCTACTACCTCTCTTACTCAACTATGTAGTCAATTAGAA
ACTTCAACAGGAGTTTTATTAAGTCCGGAAGGACTTAATCGGCGATTTAACTCGGCTTCTGTAGCCTTTTTTCGTACTGTATTTACTACACTTCTACAAG
CTAAGATTGGAGGCGTATCTAAGATTTCTCATTCTCTTTCTTCCTACTTTGAACGCATTCGCATCCTTGATTCTACAACCTTTCAAGTTCCAGATCGATT
CGCAGCTATTTATCCTGGTGCCGGAGGATGTAGTCATAAAGCTGGTGTGAAAATTGAACCAGGAAAGCGAAGTGATCAGGCGTATGGTGCGACTCGAACA
GACATGATACAAAAGAATGAGCTGTATATCCGTGACTTAGGATATTTTCGTCTACAAGACTTTAAGTCTATTCAAGACAAGCAGGGGTATTATTTATCCC
GTCTTAAGTTACCAACTAAAATATATAGAAAAGAGTTTGAAACAGTCGTATTTAAAACAAAACCTCCTCAATTGAAACCAGTATATACACAAATTCATTT
GGAAAACATTATGAAACAACTACAACCTGGCCAAGTTTATGAATTACACGATGTATATGTAGGGAGTAAAGACAAACTGCCTACTCGTATTGTAGTTTAT
AGATGTACCGAGGAGCAAAAACAGAAACGCCTACGCGATCAAACTATTCGTGAAAAGAAAAAAGGAATTACATATACAGAGCGCACGAAGCTTTTACAAG
GTATCACGGTATATATGACAAACATTCCTACGGAATGGGTACCAAAAGAGAAAATCTATGCCTTATATTCATTACGTTGGCAAATTGAGCTGTTATTTAA
AATATGGAAATCTTGGTTTCAAATTCATCGTTGTAAATCTATTAAACAAGAGCGATTAGAATGTCATCTTTATGGGCAACTTATTAGCATTCTATTTTGT
TCTTCTACTATGTTTAAAATGCGAGAATTCCTATTACGTAAGAAACAGAAAGAACTAAGTGAATATAAAGCGATGTATATAATTAAGGATTATTTTTCAC
TTTTTTACCAATCATTACACAAAAACACCCAAGAATTATCAAAGGTTCTCCTTCGTCTGTTTAACCTCCTACAGCACAACGGACAAAAATCTCATCGGTA
CGAGAAAAAGACAGTCTTTGATATTTTGGGTGTGGTTTATGAGTACACCACTTCTAATCATCAGGTAGCATAGTAAAAAATTGAAACCCGTCAGGGTTTA
TTTGATATGCCTACTTTTATAAAATCTACAAAAGATAATAAAAAATTATATAAAAAAACCACATTTTGTTTAAAAATATCCCTTAAGTTGATGGATG
ATCAAAAACAAGAATTATCTTTATTCGCTGAAGAGTTATATCGATATATGTCTCCCGCTACACTTAATCAATTAGCTATAGAAGCAGGCGGGATGAAACG
AAAACGTAAGTGCCACGGGCATCATTTTTTATCTTTGTGTGTATGGTTAAATCAACAAGTCGCTACTACCTCTCTTACTCAACTATGTAGTCAATTAGAA
ACTTCAACAGGAGTTTTATTAAGTCCGGAAGGACTTAATCGGCGATTTAACTCGGCTTCTGTAGCCTTTTTTCGTACTGTATTTACTACACTTCTACAAG
CTAAGATTGGAGGCGTATCTAAGATTTCTCATTCTCTTTCTTCCTACTTTGAACGCATTCGCATCCTTGATTCTACAACCTTTCAAGTTCCAGATCGATT
CGCAGCTATTTATCCTGGTGCCGGAGGATGTAGTCATAAAGCTGGTGTGAAAATTGAACCAGGAAAGCGAAGTGATCAGGCGTATGGTGCGACTCGAACA
GACATGATACAAAAGAATGAGCTGTATATCCGTGACTTAGGATATTTTCGTCTACAAGACTTTAAGTCTATTCAAGACAAGCAGGGGTATTATTTATCCC
GTCTTAAGTTACCAACTAAAATATATAGAAAAGAGTTTGAAACAGTCGTATTTAAAACAAAACCTCCTCAATTGAAACCAGTATATACACAAATTCATTT
GGAAAACATTATGAAACAACTACAACCTGGCCAAGTTTATGAATTACACGATGTATATGTAGGGAGTAAAGACAAACTGCCTACTCGTATTGTAGTTTAT
AGATGTACCGAGGAGCAAAAACAGAAACGCCTACGCGATCAAACTATTCGTGAAAAGAAAAAAGGAATTACATATACAGAGCGCACGAAGCTTTTACAAG
GTATCACGGTATATATGACAAACATTCCTACGGAATGGGTACCAAAAGAGAAAATCTATGCCTTATATTCATTACGTTGGCAAATTGAGCTGTTATTTAA
AATATGGAAATCTTGGTTTCAAATTCATCGTTGTAAATCTATTAAACAAGAGCGATTAGAATGTCATCTTTATGGGCAACTTATTAGCATTCTATTTTGT
TCTTCTACTATGTTTAAAATGCGAGAATTCCTATTACGTAAGAAACAGAAAGAACTAAGTGAATATAAAGCGATGTATATAATTAAGGATTATTTTTCAC
TTTTTTACCAATCATTACACAAAAACACCCAAGAATTATCAAAGGTTCTCCTTCGTCTGTTTAACCTCCTACAGCACAACGGACAAAAATCTCATCGGTA
CGAGAAAAAGACAGTCTTTGATATTTTGGGTGTGGTTTATGAGTACACCACTTCTAATCATCAGGTAGCATAGTAAAAAATTGAAACCCGTCAGGGTTTA
TTTGATATGCCTACTTTTATAAAATCTACAAAAGATAATAAAAAATTATATAAAAAAACCACATTTTGTTTAAAAATATCCCTTAAGTTGATGGATG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1380 bp | 460 aa | 91 | 1470 | + | No |
Chemistry : DDE
ORF sequence :
MNMNQKQELSLFAEELYRYMSPATLNQLAIEAGGMKRKRKCHGHHFLSLCVWLNQQVATTSLTQLCSQLETSTGVLLSPEGLNRRFNSASVAFFRTVFTT
LLQAKIGGVSKISHSLSSYFERIRILDSTTFQVPDRFAAIYPGAGGCSHKAGVKIEPGKRSDQAYGATRTDMIQKNELYIRDLGYFRLQDFKSIQDKQGY
YLSRLKLPTKIYRKEFETVVFKTKPPQLKPVYTQIHLENIMKQLQPGQVYELHDVYVGSKDKLPTRIVVYRCTEEQKQKRLRDQTIREKKKGITYTERTK
LLQGITVYMTNIPTEWVPKEKIYALYSLRWQIELLFKIWKSWFQIHRCKSIKQERLECHLYGQLISILFCSSTMFKMREFLLRKKQKELSEYKAMYIIKD
YFSLFYQSLHKNTQELSKVLLRLFNLLQHNGQKSHRYEKKTVFDILGVVYEYTTSNHQVA
LLQAKIGGVSKISHSLSSYFERIRILDSTTFQVPDRFAAIYPGAGGCSHKAGVKIEPGKRSDQAYGATRTDMIQKNELYIRDLGYFRLQDFKSIQDKQGY
YLSRLKLPTKIYRKEFETVVFKTKPPQLKPVYTQIHLENIMKQLQPGQVYELHDVYVGSKDKLPTRIVVYRCTEEQKQKRLRDQTIREKKKGITYTERTK
LLQGITVYMTNIPTEWVPKEKIYALYSLRWQIELLFKIWKSWFQIHRCKSIKQERLECHLYGQLISILFCSSTMFKMREFLLRKKQKELSEYKAMYIIKD
YFSLFYQSLHKNTQELSKVLLRLFNLLQHNGQKSHRYEKKTVFDILGVVYEYTTSNHQVA
Blast result :
Comments
ISBce9 is 93% aa similar to IS231S.
ISBce9 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-54-D(N3)-150-E(C1). ISBce9 is located on plasmid pE33L466. It's copy number in Bacillus cereus E33L is 1.
ISBce9 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-54-D(N3)-150-E(C1). ISBce9 is located on plasmid pE33L466. It's copy number in Bacillus cereus E33L is 1.
References
1] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18