ISBce8

  • Family IS4
  • Group IS231
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_003909 ND Bacillus cereus
Bacillus cereus ATCC 10987
DNA section
IS Length : 2470 bp

Ends


IR Length : 16/18

IRL : CATCGCTATCAAGCTTAGATTTTAGAGTACCCCCATAACAAAAATTTCAC
IRR : CATTGCTATCAAGCTAAGCTCATACAACGTCAATTAAAGTAGAGGTTGTT

Insertion site


Left flankDirect repeatRight flankDR Length
GGTAGCACCATGGTGCTACC0

DNA sequence

CATCGCTATCAAGCTTAGATTTTAGAGTACCCCCATAACAAAAATTTCACCTTATATACTCCAAGATAGATTAAAAATATAATAGTTATTTAAAAAAGAT
GGTGTGAATTTAGAAATCATTCCATCTTTTATTATTATCTTATACAATAAAGAAAGAATATTTTGGTAATTAAATTAAGAAAAGGGTAGAAAGGGAGAGA
AACATATGGTTAAATTGGAGTATTTTACAGAAGAAGATTTTCAACAATTAATTGATTGGATACCAAATGCGGAATTTAGTTTACAATGGGGTGGACCTGC
TTTTACATTTCCATTAACAAATGCACAATTAAAAAATTATTTACAAAACGCTAATAAAGATAATGCTATTAAATATGTATTTAAAGCAATAGACGAAACA
AATAGTGAAGTCATTGGTCATATTTCATTGGGAAACGTCGACAAAACTAATGAAAGTGCAAGGATAGGAAAAGTATTAATTGGCTCAACAAATTCAAGAG
GTAAAGGTTATGGAACACAAATGATGACAGCTGTTTTAAAATTTGCTTTTGAAGAATTAAAATTACATAAAGTAACTCTAGGTGTATTCGATTTTAATGA
ATCTGCAATTAAATGTTATAAAAAAGTCGGCTTTCAACAAGAAGGATTCCTACGTGATGCTAGAAAAAGTGGTCAAGAATATTGGAATTTAATTGAAATG
GGTATCTTATACAGCGAGTGGAAAATAAATAATTAAAAATAAGAAGGACTCTTAACTATTCACTTATCAAATTAAATATTTTTAAAATATTTTTATATAC
GGATTATATCTTTAATAAAAAAGTTAGTTTGGTAGCAAAAAGAAATATCCTCAAAATAAAAAATACGTTATTCTTTCTGTAGAATCATAGGAAAGGATGG
CGTTTTTTGTATGTCTATTTCTGTATCTGACAAACTACAACTATTTGCACAGGAGATTCAGAGTCTTTTATCCCCAAATACCTTACAAAATCTAGCTAGA
GATGTTGGGTTTGTACAGCGAGCAAGTAAGTATCGAGCACAAGATTTAATAGCTTTATGTGTATGGATAAGTCAAAGTGTAGCAAAAACTTCTTTAACTC
AGTTATGTAGCTGTTTAGAAGCATCAACAGAAGTTCTCATCAGTCCCGAAGGACTGAATCAACGATTTAATGCCACAGCCGTACAATTTCTACAACAAGT
GTTAGCCGAACTTCTCAACCAAAAGTTATCTTCAACTAAGCTGTTCTCTTCTCCACACACTTCTGTTTTCAAGCGTATTCGTATTTTAGATTCAACTGCA
TTTCAACTTCCAGATTCTTTTCCATTCGTTTATCCAGGTGCAGGAGGATGCAGCCATACAGCGGGAGTAAAGATTCAGCTTGAGTATGATTTATTAAGTG
GACAGTTCCTTCATATTCATACAGGTCCAGGTAAACAACATGATCGAACCTACGGTTCCTTGTGTGTCCCAACTGTAACAGCGAATGATTTATGTATCCG
AGATTTAGGTTATTTTCATTTGAAAGATCTTCAACATATACAAGATAAAAAGGCTTATTATATCTCACGTATTAAATCGAATACACGTATTTATCAAAAA
AATCCTAAACCCGATTATTTTCAAGATGGCAGAATCAAGAAAGGTACAGAGTATATCCAGATAGATATGGAGATTTTAATGAACTCTCTTCAGCCAGGAC
AAACATGCGAAATATCCAACGCTTATGTAGGAATGACTGATAAAGTCCCAACTCGTGTGATTGTTCATCGACTAACAAAAGAACAACAACAAAAACGATT
ACGCGATCAAACTGTAAGAGAAAAAAAGAAAGGAATGAAGTATTCTGCTCGTAGTAAACGGCTTAGTGGTATCAATGTATATATGACAAATACTCCTATA
GATATTGTCCCAATGGGACAAGTACATGATTGGTATTCTTTACGTTGGCAAATCGAGATTTTATTTAAAACGTGGAAATCATTCTTTCATATACATCATT
GTAAAAAGATAAAACGAGAACGATTGGAATGCCATTTGTATGGACAACTGATTGCCATTTTACTTTGTTCTTCTACCATGTTTCAAATGCGACAATTGCT
TCTTATGAAAAAGAAACGAGAACTGAGTGAATATAAGTCCATATATATGATTAAAGATTACTTTCTTCTTCTTTTCCAAGCAATACAGAAAGACACCCAA
GAGCTATCAAAGATTCTAATTCGCCTGTTCAACCTCCTACAGCAAAACGGGAGAAAATCTCATCGATATGAGAAGAAAACAGTCTTTGATATATTAGGTG
TCGTTTACAATTGTACCATGTCTGATAATCAAGCGGCTTAATTCAAAAAAATGAAACCCGTTAGGGTTTATTTCGTGTGCAAATCTTTAGGTTCCCTACA
CATAAATTTTATAAAAAAGAAACAACCTCTACTTTAATTGACGTTGTATGAGCTTAGCTTGATAGCAATG
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1428 bp476 aa9112338+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSISVSDKLQLFAQEIQSLLSPNTLQNLARDVGFVQRASKYRAQDLIALCVWISQSVAKTSLTQLCSCLEASTEVLISPEGLNQRFNATAVQFLQQVLAE
LLNQKLSSTKLFSSPHTSVFKRIRILDSTAFQLPDSFPFVYPGAGGCSHTAGVKIQLEYDLLSGQFLHIHTGPGKQHDRTYGSLCVPTVTANDLCIRDLG
YFHLKDLQHIQDKKAYYISRIKSNTRIYQKNPKPDYFQDGRIKKGTEYIQIDMEILMNSLQPGQTCEISNAYVGMTDKVPTRVIVHRLTKEQQQKRLRDQ
TVREKKKGMKYSARSKRLSGINVYMTNTPIDIVPMGQVHDWYSLRWQIEILFKTWKSFFHIHHCKKIKRERLECHLYGQLIAILLCSSTMFQMRQLLLMK
KKRELSEYKSIYMIKDYFLLLFQAIQKDTQELSKILIRLFNLLQQNGRKSHRYEKKTVFDILGVVYNCTMSDNQAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
528 bp176 aa206733+No
ORF function : Passenger Gene
Annotation : Description :

ORF sequence :

MVKLEYFTEEDFQQLIDWIPNAEFSLQWGGPAFTFPLTNAQLKNYLQNANKDNAIKYVFKAIDETNSEVIGHISLGNVDKTNESARIGKVLIGSTNSRGK
GYGTQMMTAVLKFAFEELKLHKVTLGVFDFNESAIKCYKKVGFQQEGFLRDARKSGQEYWNLIEMGILYSEWKINN

 

Blast result :
Comments
ISBce8 is 95% aa similar to IS231Y.
ISBce8 was found by screening completely sequenced genomes for sequences homologous to the IS4 transposase using BLASTP. Multiple sequence alignments revealed a conserved DDE motif : D(N2)-70-D(N3)-150-E(C1). The copy number in Bacillus cereus ATCC 10987 is 1.
In addition to the transposase, this element carries an ORF (ORF2) coding for a protein similar to members of the GCN5-related histone N-acetyltransferase (GNAT) superfamily, which possibly inactivates antibiotics of the aminoglycoside family.
References
1] Rasko,D.A., Ravel,J., Okstad,O.A., Helgason,E., Cer,R.Z., Jiang,L., Shores,K.A., Fouts,D.E., Tourasse,N.J., Angiuoli,S.V., Kolonay,J., Nelson,W.C., Kolsto,A.-B., Fraser,C.M. and Read,T.D.(2004) Nucleic Acids Res. 32(3), 977-988
2] De Palmenaer D, Siguier P, Mahillon J (2008) BMC Evol Biol , 8(1):18