ISRle3

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008382 ND Rhizobium leguminosarum
Rhizobium leguminosarum bv. viciae 3841 plasmid pRL7
DNA section
IS Length : 2500 bp

Ends


IR Length : 14/15

IRL : GTAAGCGACAAGCTGACCCCATCTGGCGTCGGTAGCGTTGGCCGCTGTAT
IRR : GTAAGCGACAAGCCGGGGCCGTTCAGGCGGCGTAGTTCCATGGCATGAGG

Insertion site


Left flankDirect repeatRight flankDR Length
AGTCGCGGACAATTTGCAGGGTCACGCCTCCG8
AGTCGCGGACAATTTGCAGGGTCACGCCTCCG8

DNA sequence

GTAAGCGACAAGCTGACCCCATCTGGCGTCGGTAGCGTTGGCCGCTGTATTGCGGACAGACGATTCACAAACTGTGAGCTTCTATGTCTGCGCCTAGCTC
TGGAACTAGAAACTTCCATATGATTGAGGCTGTTCCGGAACGGCTTGAGGGCGCCCCACGGCAATTTCGGCGCCGCTGGTCCGATGATTTTAAAGCGCAG
GCTGTGGCTGAGGCAATGGAGCCCGGCGCAAGCGTCTCGGCCATTGCGCATCGCATCGGCATACATCCCTCGCAGCTATTTGGCTGGCGTCGTGATGCTC
GTGACGGACAACGATCCTTCTCGCAAGATCGGGCTGGTCAGACAGGGACCAGGACGATTGGCACACGTGCGATGATCGAGCTTGTGATCGGCGACGTCAT
TATCCGTGCCGACGCAGATATCGGCGAAGCACAGCTGCAGCGGGTGATCCGAGCGGTGCGGTCGGCATGATCCCGTCCGGCGTGAAGGTCTTTCTTGCGA
GCCATCCCATCGACTTCCGAAAGGGGCCGGACAGCTTGCTTTCGCTGGTACGGGATGCTGGCAGTGATCCGTTCAACGGTGCACTTTATGTCTTTCGGGC
CAAGAGAGCAGACCGAGTCAAGATCGTGTGGTGGGATGGCTCCGGCGTTTGCCTCTATGCGAAGCGGCTGGAAAAAGCCCAGTTCTGCTGGCCCCGGATC
GGCCATCATCGCGTCCAGCTCAATCATGCCCAGCTTCTGGCTTTGGTCGACGGAATGGACTGGAAGCGAGTTCGATCCGCGCCGGTGAAGCCTCCCGAGA
TTGTTGGGTAAAACCGCTGCGGCGAAGTGAATCAGGTCCCTGAAAGCATGGGCGGAGCGCGGCAAAATATGCTCTGATCATACCCATGACGCGGCCCGAG
ATTGAGCTCCCGGATGATGTAGAGGCTCTCAAAGCCATGGTTCTTGCCATGGCTGCAAAAGCAGCCCGCGTCGAGGCTTTGGAGAAACAGGTTGACGATC
TAGAGGCCCGCAACGCGGACGCCGATGAGCGCATCGAGCGGCTGACGCAGATCCTGAAGGCTTTCGATCGCGCCCGATTTGGCCGACGCTCGGAAAGGCT
TGCATCATCCACCGTCGATGACGAGCAGCATGCCTTTGTCTTCGAAGAGATCGAGACCGGCATTGCGACGATCAAGGCTCAGGTCACGAAAGGCCGCGGT
AGCGCGGACAGCAAACGCGCTCCGCGGCCACGCAAGGGCTTTGCGCCTCATCTTGAGCGCGTCGAGGTCGTGATCGAACCGGAAGAGCTTCCAGAACATG
CAGGTAAGACCAAGATCCTGATTGGCGAAGACGTCTCCGAGCGGCTGGATGTCGTGGCAGCGAAGTTCCGTGTCATCGTCACCCGCCGGCCGAAGTATGC
CTTTAGGAACGAGGATGGTGTCGTCCAGGCGGCGGCTCCGGCACACATCATCGAAGGTGGCATTCCCACGGAAGCACTTCTGGCCCAGATCGCCGTTTCC
AAATATGCCGACGGGCTGCCACTCTATCGCCAGGAAGCCATCTACGCACGCGACAAGGTCGAACTCGACCGCAAGCTAATGGCTCAATGGATGGGCAAGC
TGGGGTTCGAGCTCAATATCCTCGCCGACTACATCCTCGATGAGATCAAGAAGGCCGAACGGATCTTTGCCGACGAAACGACCTTGCCCACACTTGCTCC
TGGTTCCGGATCAGCGAAGACGGCATGGCTATGGGCGTATGCCAGGGATGACCGGACTTTTGGGGGCAGCGGTCCACCGATGGTAGCCTATCGCTTCGAG
GACAGTCGAGCCACCGAGTGTGTCGCACGACACCTGAACGGCTATCGTGGAATCCTGCAGGTCGATGGATATGCCGCTTATAACAAGCTCGTCCGGAAAG
ATGGAGGCAATAACAGCGCCATCCTGGCCGGCTGTTGGTCGCACAGCCGGAGAAAGTTCTACGAGTTGCATGTCGCAAAGAGCTCGAAAGTCGCTACCGA
CACCGTCGAGCGTATGGCGAGGTTATGGGAGATCGAGGAGCGTGTGCGCGGCCAAAGCCCTGAAGCTCGTGTCGCTGCACGCCAGGAGATCTCCGCAGCG
ATCGTCCGTGACCTCTTCACTCTCTGGCAGGCGACCCTGCCGCGGGTCTCTGGAAAATCCAAACTCGCCGAGGCTCTGCGTTATGCCATCTCGCGTCGAG
ACATCTTCGAGCGCTTCCTGACCGACGGCCGCATCGAACTCGACTCCAACATAGTCGAGCGAGCAATCAGACCCCAAGCGATCACGAGAAAAAATAGTCT
ATTCGCTGGAAGCGACGGTGGCGGCCGGACTTGGGCGACCATCGCGACGCTCCTGCAAACGGCAAAGATGAATGCCGTCGATCCGCAAGCCTGGTTGACC
CAGACGCTTGAGCGCCTTGCGAATGGATGGCCCAACAGTGAAATCGACGCCCTCATGCCATGGAACTACGCCGCCTGAACGGCCCCGGCTTGTCGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
387 bp128 aa84470+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MSAPSSGTRNFHMIEAVPERLEGAPRQFRRRWSDDFKAQAVAEAMEPGASVSAIAHRIGIHPSQLFGWRRDARDGQRSFSQDRAGQTGTRTIGTRAMIEL
VIGDVIIRADADIGEAQLQRVIRAVRSA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
345 bp114 aa467811+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIPSGVKVFLASHPIDFRKGPDSLLSLVRDAGSDPFNGALYVFRAKRADRVKIVWWDGSGVCLYAKRLEKAQFCWPRIGHHRVQLNHAQLLALVDGMDWK
RVRSAPVKPPEIVG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1593 bp530 aa8862478+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTRPEIELPDDVEALKAMVLAMAAKAARVEALEKQVDDLEARNADADERIERLTQILKAFDRARFGRRSERLASSTVDDEQHAFVFEEIETGIATIKAQV
TKGRGSADSKRAPRPRKGFAPHLERVEVVIEPEELPEHAGKTKILIGEDVSERLDVVAAKFRVIVTRRPKYAFRNEDGVVQAAAPAHIIEGGIPTEALLA
QIAVSKYADGLPLYRQEAIYARDKVELDRKLMAQWMGKLGFELNILADYILDEIKKAERIFADETTLPTLAPGSGSAKTAWLWAYARDDRTFGGSGPPMV
AYRFEDSRATECVARHLNGYRGILQVDGYAAYNKLVRKDGGNNSAILAGCWSHSRRKFYELHVAKSSKVATDTVERMARLWEIEERVRGQSPEARVAARQ
EISAAIVRDLFTLWQATLPRVSGKSKLAEALRYAISRRDIFERFLTDGRIELDSNIVERAIRPQAITRKNSLFAGSDGGGRTWATIATLLQTAKMNAVDP
QAWLTQTLERLANGWPNSEIDALMPWNYAA

 

Blast result :
Comments
ISRle3 is 91%(ORF1), 84%(ORF2) and 93%(ORF3) aa similar to IS292.
References
1] Young,J.W., Crossman,L.C., Johnston,A.W.B., Thomson,N.R., Ghazoui,Z.F., Hull,K.H., Wexler,M., Curson,A.R.J., Todd,J.D., Poole,P.S., Mauchline,T.H., East,A.K., Quail,M.A., Churcher,C., Arrowsmith,C., Cherevach,A., Chillingworth,T., Clarke,K., Cronin,A., Davis,P., Fraser,A., Hance,Z., Hauser,H., Jagels,K., Moule,S., Mungall,K., Norbertczak,H., Rabbinowitsch,E., Sanders,M., Simmonds,M., Whitehead,S. and Parkhill,J. (2006) Genome Biol. 7, R4-R4