ISMmy3
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Mycoplasma mycoides | Mycoplasma mycoides subsp mycoides LC |
DNA section
IS Length : 1442 bp
Ends
IR Length : 17/24
IRL : TGCATAGTACCCCCTTGTCCGGACAAGGGGGTACTATTTTTATGGCTAAA
IRR : TGCTCGGTACCCCGAAATCCGGAATTTTTTAAGCGGTAGCCTGTCCGGTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNNNNNNN | TTT | NNNNNNNNNN | 3 |
DNA sequence
TGCATAGTACCCCCTTGTCCGGACAAGGGGGTACTATTTTTATGGCTAAATATTTATACGAGATTAAAAAGAAAATCATAAACGATTTAGTGAATGGTAA
GACTATTACTCAAGTGTCTAGAGAGTATGGCATAAACATCAATACAATAGTGAATTGAAAGAACCAATATGAATCTTTTGGTATTAGAGGTATTAGAAAA
AGTAAGAATAAAACTATATATGCTTCTGAATTTAAAATTGCTGTTATAAAATATAAAAACGAAAATAATTTAGGTTTTTTAGAAACAGCAAAACATTTTA
ATATAAAGAATGCATCAACAATAGCTTTATGAGAAAACAAATATTATAAACACGGTGTTGCTGGTATTTCTAAATTAATAGGAAATGCAGGAGATATTGA
TAGCATGCCGTCGAACAAAAACAAATCAATAGAAACAGAAGATAAAACTAAAGAACAATTAGCTCATGAACTAGAATTAGCAAAAGCTGAGATTGCATAT
TTAAAAAAGTTGAGAGCTCTAATTCATTCAAAACCAAAGAGAAAGTAAAATCAATTTTAGAATTAGAGCAAAACTATCCAAATTTAAAAACATCTAATTG
ATTAGAAATTGCTAATATCAAAAAATCTACATTTTATGAGTGAAAGCAAAAATTAAGGCAAAATATCCATAATAACGATAAATATGGTGAAATAACTTTA
AAAATAGAAGAGATTTTCTACGATAATGACCGTGATTTTGGCCATAAACGCATACATAGTCAATTAAAGTTTCTAGGTTATAAAATCAGCAGAAAGAAAG
TATTGCAAATAATGAAAGAAAACGGCTGGATTTCTATTTATAACGCTTGTAGGCGTCGAAAATACAGTTCATACAAAGGAAATGTAGGAACTATTGCTAA
TAACGTAATAAAAAGAGATTTCAAAGCATATAAGCCGTTTTCAAAAATATTTGGTGATTTAACAGAATTAAAGATTGGCGAACAAAAACTATATTTACAA
ATCTATAAAGATACATTTAACAATCAAATTGTTGGTTATAGTTATGGAACTAGACCAACTGTTGAAATGACAAATAAAGCTCTTGATAGTTTTATAGACA
AAATAATTCCTGGCGAAACTATAATACATACTGATCAAGGAATTCATTACCAACATGGAACATTTGTTGAAAAATTAAAAAAAGCAGGTGCTATTCAAAG
TATGTCTAGAAAGGGAAATTGTCTAGATAACTCACCTGCAGAAAATTTCTTTAGTATATTAAAAAGAGAAATGTTCTATAATAGAAAATATAAAAATATT
GATCAAGTTATTGAGAAATTAAACTGATATATTCATTGATATAATAATAGAAGAATTAGCTTAAAACTAAGCGGATTAACACCTGTTCAATATACCGGAC
AGGCTACCGCTTAAAAAATTCCGGATTTCGGGGTACCGAGCA
GACTATTACTCAAGTGTCTAGAGAGTATGGCATAAACATCAATACAATAGTGAATTGAAAGAACCAATATGAATCTTTTGGTATTAGAGGTATTAGAAAA
AGTAAGAATAAAACTATATATGCTTCTGAATTTAAAATTGCTGTTATAAAATATAAAAACGAAAATAATTTAGGTTTTTTAGAAACAGCAAAACATTTTA
ATATAAAGAATGCATCAACAATAGCTTTATGAGAAAACAAATATTATAAACACGGTGTTGCTGGTATTTCTAAATTAATAGGAAATGCAGGAGATATTGA
TAGCATGCCGTCGAACAAAAACAAATCAATAGAAACAGAAGATAAAACTAAAGAACAATTAGCTCATGAACTAGAATTAGCAAAAGCTGAGATTGCATAT
TTAAAAAAGTTGAGAGCTCTAATTCATTCAAAACCAAAGAGAAAGTAAAATCAATTTTAGAATTAGAGCAAAACTATCCAAATTTAAAAACATCTAATTG
ATTAGAAATTGCTAATATCAAAAAATCTACATTTTATGAGTGAAAGCAAAAATTAAGGCAAAATATCCATAATAACGATAAATATGGTGAAATAACTTTA
AAAATAGAAGAGATTTTCTACGATAATGACCGTGATTTTGGCCATAAACGCATACATAGTCAATTAAAGTTTCTAGGTTATAAAATCAGCAGAAAGAAAG
TATTGCAAATAATGAAAGAAAACGGCTGGATTTCTATTTATAACGCTTGTAGGCGTCGAAAATACAGTTCATACAAAGGAAATGTAGGAACTATTGCTAA
TAACGTAATAAAAAGAGATTTCAAAGCATATAAGCCGTTTTCAAAAATATTTGGTGATTTAACAGAATTAAAGATTGGCGAACAAAAACTATATTTACAA
ATCTATAAAGATACATTTAACAATCAAATTGTTGGTTATAGTTATGGAACTAGACCAACTGTTGAAATGACAAATAAAGCTCTTGATAGTTTTATAGACA
AAATAATTCCTGGCGAAACTATAATACATACTGATCAAGGAATTCATTACCAACATGGAACATTTGTTGAAAAATTAAAAAAAGCAGGTGCTATTCAAAG
TATGTCTAGAAAGGGAAATTGTCTAGATAACTCACCTGCAGAAAATTTCTTTAGTATATTAAAAAGAGAAATGTTCTATAATAGAAAATATAAAAATATT
GATCAAGTTATTGAGAAATTAAACTGATATATTCATTGATATAATAATAGAAGAATTAGCTTAAAACTAAGCGGATTAACACCTGTTCAATATACCGGAC
AGGCTACCGCTTAAAAAATTCCGGATTTCGGGGTACCGAGCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 507 bp | 168 aa | 42 | 548 | + | No |
Description : First part of the transposase
ORF sequence :
MAKYLYEIKKKIINDLVNGKTITQVSREYGININTIVNWKNQYESFGIRGIRKSKNKTIYASEFKIAVIKYKNENNLGFLETAKHFNIKNASTIALWENK
YYKHGVAGISKLIGNAGDIDSMPSNKNKSIETEDKTKEQLAHELELAKAEIAYLKKLRALIHSKPKRK
YYKHGVAGISKLIGNAGDIDSMPSNKNKSIETEDKTKEQLAHELELAKAEIAYLKKLRALIHSKPKRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 966 bp | 321 aa | 449 | 1414 | + | No |
Description : Second part of the transposase
ORF sequence :
RTISSWTRISKSWDCIFKKVESSNSFKTKEKVKSILELEQNYPNLKTSNWLEIANIKKSTFYEWKQKLRQNIHNNDKYGEITLKIEEIFYDNDRDFGHKR
IHSQLKFLGYKISRKKVLQIMKENGWISIYNACRRRKYSSYKGNVGTIANNVIKRDFKAYKPFSKIFGDLTELKIGEQKLYLQIYKDTFNNQIVGYSYGT
RPTVEMTNKALDSFIDKIIPGETIIHTDQGIHYQHGTFVEKLKKAGAIQSMSRKGNCLDNSPAENFFSILKREMFYNRKYKNIDQVIEKLNWYIHWYNNR
RISLKLSGLTPVQYTGQATAZ
IHSQLKFLGYKISRKKVLQIMKENGWISIYNACRRRKYSSYKGNVGTIANNVIKRDFKAYKPFSKIFGDLTELKIGEQKLYLQIYKDTFNNQIVGYSYGT
RPTVEMTNKALDSFIDKIIPGETIIHTDQGIHYQHGTFVEKLKKAGAIQSMSRKGNCLDNSPAENFFSILKREMFYNRKYKNIDQVIEKLNWYIHWYNNR
RISLKLSGLTPVQYTGQATAZ
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1373 bp | 457 aa | 42 | 1414 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKYLYEIKKKIINDLVNGKTITQVSREYGININTIVNWKNQYESFGIRGIRKSKNKTIYASEFKIAVIKYKNENNLGFLETAKHFNIKNASTIALWENK
YYKHGVAGISKLIGNAGDIDSMPSNKNKSIETEDKTKEQLAHELELAKAEIAYLKKVESSNSFKTKEKVKSILELEQNYPNLKTSNWLEIANIKKSTFYE
WKQKLRQNIHNNDKYGEITLKIEEIFYDNDRDFGHKRIHSQLKFLGYKISRKKVLQIMKENGWISIYNACRRRKYSSYKGNVGTIANNVIKRDFKAYKPF
SKIFGDLTELKIGEQKLYLQIYKDTFNNQIVGYSYGTRPTVEMTNKALDSFIDKIIPGETIIHTDQGIHYQHGTFVEKLKKAGAIQSMSRKGNCLDNSPA
ENFFSILKREMFYNRKYKNIDQVIEKLNWYIHWYNNRRISLKLSGLTPVQYTGQATA
YYKHGVAGISKLIGNAGDIDSMPSNKNKSIETEDKTKEQLAHELELAKAEIAYLKKVESSNSFKTKEKVKSILELEQNYPNLKTSNWLEIANIKKSTFYE
WKQKLRQNIHNNDKYGEITLKIEEIFYDNDRDFGHKRIHSQLKFLGYKISRKKVLQIMKENGWISIYNACRRRKYSSYKGNVGTIANNVIKRDFKAYKPF
SKIFGDLTELKIGEQKLYLQIYKDTFNNQIVGYSYGTRPTVEMTNKALDSFIDKIIPGETIIHTDQGIHYQHGTFVEKLKKAGAIQSMSRKGNCLDNSPA
ENFFSILKREMFYNRKYKNIDQVIEKLNWYIHWYNNRRISLKLSGLTPVQYTGQATA
Blast result :
Comments
ISMmy3 is 53% (ORF 1) and 63% (ORF2) aa similar to IS1296. The third ORF is a fusion in silico, there is a potential frameshift at position 503 to 510 (AAAAAAG).
References
1] Thiaucourt, F. (2006) Direct submission.