ISStso1
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AEUV02000002 | ND | Streptococcus sobrinus | Streptococcus sobrinus Streptococcus criceti HS-6 |
DNA section
IS Length : 1091 bp
Ends
IR Length : 17/22
IRL : CTTAACAAAAATCAAAAATAGCGATATAATATAGTCATGACACTAGAAAT
IRR : CTCAATGAAAATCAAGCATAGCCGAAGTCCATTTTCTATGGACAATCGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAAGTGGTAA | TA | TAATGAAAAA | 2 |
DNA sequence
CTTAACAAAAATCAAAAATAGCGATATAATATAGTCATGACACTAGAAATTACACCAGCACAAGCACAAGAACTAAAACAAGCACTCAAAGATAAAGCAA
ATGATAAACACTATAGAAAACTTCATGCCCTCCTCCTTCGTTCACAAGGAATGAGCTTAACAGCAATCGGCAAAGAAGTTGGGCTCGTTCACCAGTCTGT
TCGCAACTTAATTACCCGTTATCAAAAGGGGGGACTAACAGCTCTATTTAAAGAAAATCGCGGTGGTCGTAGACGTGCTTATATGACCGTCGAAGAGGAA
GAAAGATTCCTAAACCAACAACTAGAACGGGCATTAAAGGGGGAGCACGTAACTGTTCAAAGCTTGTTTGAAGCCTATCAAGCTGAAGTTGGAAAGTCAA
CCACTCGTGAGGGATTCTATGCTTTATTAAAACGCCACGGATGGCGAAAGGTAACTCCTCGTCCAAAACATCCTAAAAAAGCAGACGCTAAAACGATTTA
TGCGTCAAAAAATAAAATCTATATTCAGGAAGACAAGAAAGCGCTTTAAGCATAGCCGACGTTACAAGAAAGTCCGTCTCATGTATCAAGATGAAGCGGG
TTTTGGTCGTATCAGTAAGCTAGGAGCTTGTTGGGCACCTAAAGGTTATAGGCCACATGTAGCCAGCCACTACATTCGAGAATACCGTTATTGTTACGGA
GCCGTTGATGCCCATACAGGAGAATCTTTTTTCCTCATCGCAGGAGGTTGTAATACTGAATGGATGAACGAATTTTTAAGACAATTAAGTCAAGCTTTTC
CTGACGATTACATTGTTTTGGTTATGGATAATGCCATTTGGCATAAATCAAAGGCTTTAGAAGTTCCACATAATATAGACTTTGCTTTTATTCCGCCTTA
TACTCCTGAAATGAATCCTATTGAACAGGTCTGGGCTGAAATAAGAAAACGTGGGTTTAAAAACAAAGCCTTCAAAACTTTGGAGGAGGTTATTAATCAA
CTTCAAGAGGTTATTCAAAGTTTTCATTGGAAAGAGTTAAAAACGATTGTCCATAGAAAATGGACTTCGGCTATGCTTGATTTTCATTGAG
ATGATAAACACTATAGAAAACTTCATGCCCTCCTCCTTCGTTCACAAGGAATGAGCTTAACAGCAATCGGCAAAGAAGTTGGGCTCGTTCACCAGTCTGT
TCGCAACTTAATTACCCGTTATCAAAAGGGGGGACTAACAGCTCTATTTAAAGAAAATCGCGGTGGTCGTAGACGTGCTTATATGACCGTCGAAGAGGAA
GAAAGATTCCTAAACCAACAACTAGAACGGGCATTAAAGGGGGAGCACGTAACTGTTCAAAGCTTGTTTGAAGCCTATCAAGCTGAAGTTGGAAAGTCAA
CCACTCGTGAGGGATTCTATGCTTTATTAAAACGCCACGGATGGCGAAAGGTAACTCCTCGTCCAAAACATCCTAAAAAAGCAGACGCTAAAACGATTTA
TGCGTCAAAAAATAAAATCTATATTCAGGAAGACAAGAAAGCGCTTTAAGCATAGCCGACGTTACAAGAAAGTCCGTCTCATGTATCAAGATGAAGCGGG
TTTTGGTCGTATCAGTAAGCTAGGAGCTTGTTGGGCACCTAAAGGTTATAGGCCACATGTAGCCAGCCACTACATTCGAGAATACCGTTATTGTTACGGA
GCCGTTGATGCCCATACAGGAGAATCTTTTTTCCTCATCGCAGGAGGTTGTAATACTGAATGGATGAACGAATTTTTAAGACAATTAAGTCAAGCTTTTC
CTGACGATTACATTGTTTTGGTTATGGATAATGCCATTTGGCATAAATCAAAGGCTTTAGAAGTTCCACATAATATAGACTTTGCTTTTATTCCGCCTTA
TACTCCTGAAATGAATCCTATTGAACAGGTCTGGGCTGAAATAAGAAAACGTGGGTTTAAAAACAAAGCCTTCAAAACTTTGGAGGAGGTTATTAATCAA
CTTCAAGAGGTTATTCAAAGTTTTCATTGGAAAGAGTTAAAAACGATTGTCCATAGAAAATGGACTTCGGCTATGCTTGATTTTCATTGAG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 513 bp | 170 aa | 37 | 549 | + | No |
Description : First part of the transposase
ORF sequence :
MTLEITPAQAQELKQALKDKANDKHYRKLHALLLRSQGMSLTAIGKEVGLVHQSVRNLITRYQKGGLTALFKENRGGRRRAYMTVEEEERFLNQQLERAL
KGEHVTVQSLFEAYQAEVGKSTTREGFYALLKRHGWRKVTPRPKHPKKADAKTIYASKNKIYIQEDKKAL
KGEHVTVQSLFEAYQAEVGKSTTREGFYALLKRHGWRKVTPRPKHPKKADAKTIYASKNKIYIQEDKKAL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 636 bp | 211 aa | 455 | 1090 | + | No |
Description : Second part of the transposase
ORF sequence :
LLVQNILKKQTLKRFMRQKIKSIFRKTRKRFKHSRRYKKVRLMYQDEAGFGRISKLGACWAPKGYRPHVASHYIREYRYCYGAVDAHTGESFFLIAGGCN
TEWMNEFLRQLSQAFPDDYIVLVMDNAIWHKSKALEVPHNIDFAFIPPYTPEMNPIEQVWAEIRKRGFKNKAFKTLEEVINQLQEVIQSFHWKELKTIVH
RKWTSAMLDFH
TEWMNEFLRQLSQAFPDDYIVLVMDNAIWHKSKALEVPHNIDFAFIPPYTPEMNPIEQVWAEIRKRGFKNKAFKTLEEVINQLQEVIQSFHWKELKTIVH
RKWTSAMLDFH
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1054 bp | 350 aa | 37 | 1090 | + | Yes |
Chemistry : DDE
ORF sequence :
MTLEITPAQAQELKQALKDKANDKHYRKLHALLLRSQGMSLTAIGKEVGLVHQSVRNLITRYQKGGLTALFKENRGGRRRAYMTVEEEERFLNQQLERAL
KGEHVTVQSLFEAYQAEVGKSTTREGFYALLKRHGWRKVTPRPKHPKKQTLKRFMRQKIKSIFRKTRKRFKHSRRYKKVRLMYQDEAGFGRISKLGACWA
PKGYRPHVASHYIREYRYCYGAVDAHTGESFFLIAGGCNTEWMNEFLRQLSQAFPDDYIVLVMDNAIWHKSKALEVPHNIDFAFIPPYTPEMNPIEQVWA
EIRKRGFKNKAFKTLEEVINQLQEVIQSFHWKELKTIVHRKWTSAMLDFH
KGEHVTVQSLFEAYQAEVGKSTTREGFYALLKRHGWRKVTPRPKHPKKQTLKRFMRQKIKSIFRKTRKRFKHSRRYKKVRLMYQDEAGFGRISKLGACWA
PKGYRPHVASHYIREYRYCYGAVDAHTGESFFLIAGGCNTEWMNEFLRQLSQAFPDDYIVLVMDNAIWHKSKALEVPHNIDFAFIPPYTPEMNPIEQVWA
EIRKRGFKNKAFKTLEEVINQLQEVIQSFHWKELKTIVHRKWTSAMLDFH
Blast result :
Comments
File updated September 20 2012.
ISStso1 is 75% aa similar to ISSpn2.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISStso1 is 75% aa similar to ISSpn2.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Claverys, JP. (2006) Direct submission.