ISSpn2
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003028 | ND | Streptococcus pneumoniae | Streptococcus pneumoniae TIGR4 |
DNA section
IS Length : 1089 bp
Ends
IR Length : 20/28
IRL : TACTAGACTAAAATCAAAAAGCATTATATAATAGTGATATGAAATCAACT
IRR : TACTCATCTGTTTTCAAAAAGCATTCTAGTCCATCTCCGATTAACGATGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCTCGCTATT | TA | AAAAGAAAG | 2 |
DNA sequence
TACTAGACTAAAATCAAAAAGCATTATATAATAGTGATATGAAATCAACTAAAGAAGAAATCCAAACCATCAAAACACTTTTAAAAGACTCTCGTACAGC
TAAATATCATAAACGCCTTCAAATCGTTCTATTTCGTCTGATGGGCAAATCTTATAAAGAGATTATAGAACTTTTAGACTGTAATCAAACAACGATTTGG
CGAAATGTAAAAAAATATGAGGAGTTCGGACTCGACTCTCTCCTTCAAGAAACACGTGGTGGTCGTAACCATGCATATATGACAGTTGAGGAAAAGAAAG
TCTTTCTTGCCCGCCATTTGAAGGCTGCAGAGGCAGGAGAATTTGTTACAATTGATGCCTTATTTCAGGCTTATAAAAAGGAGTTAGGTCGTTCCTACAC
ACGTGATGCCTTCTATCAACTGTTGAAGTGCCATGGTTGGCGAAATATTATGCCACGTCCAGAACATCCTAAGAAAGCAGACGCTCAAACCATTGTCGCG
TCTAAAAATAAAATCTCAATTCAAGAAGAAAAGAAAGCGCTTTAAAACCAGTAGACGTTTTCGTAAGGTTCGCTTGATGTACCAAGATGAGGCTGGTTTC
GGTAGAATCAGTAAACTGGGATCTTGTTGGGCTCCAATAGGAGTAGGTCCACATATCCATAGTCACTATATACGAGAATTTCGCTATTGTTATGGAGCTG
TTGATGCCCATACAGGCGAATCATTTTTCTTAATAGCTGGTAGATGTAATACTGAGTGGATGAACGCCTTTTTAGAAGAGCTTTCACAAGCTTATCCAGA
TGATTATCTTTTACTCGTTATGGACAATGCTATATGGCATAAATCAAGTACCTTAAAGATTCCGACTAATATTGGTTTTACCTTTATTCCTCCATACACA
CCAGAGATGAACCCCATTGAACAAGTGTGGAAAGAGATTCGTAAACGTGGATTTAAGAATAAAGCCTTTCAAACTTTGGAAGATGTCATGAATCAACTCC
AAGATGTTATACAAGGATTGGAGAAGGAGGTGATAAAGTCCATCGTTAATCGGAGATGGACTAGAATGCTTTTTGAAAACAGATGAGTA
TAAATATCATAAACGCCTTCAAATCGTTCTATTTCGTCTGATGGGCAAATCTTATAAAGAGATTATAGAACTTTTAGACTGTAATCAAACAACGATTTGG
CGAAATGTAAAAAAATATGAGGAGTTCGGACTCGACTCTCTCCTTCAAGAAACACGTGGTGGTCGTAACCATGCATATATGACAGTTGAGGAAAAGAAAG
TCTTTCTTGCCCGCCATTTGAAGGCTGCAGAGGCAGGAGAATTTGTTACAATTGATGCCTTATTTCAGGCTTATAAAAAGGAGTTAGGTCGTTCCTACAC
ACGTGATGCCTTCTATCAACTGTTGAAGTGCCATGGTTGGCGAAATATTATGCCACGTCCAGAACATCCTAAGAAAGCAGACGCTCAAACCATTGTCGCG
TCTAAAAATAAAATCTCAATTCAAGAAGAAAAGAAAGCGCTTTAAAACCAGTAGACGTTTTCGTAAGGTTCGCTTGATGTACCAAGATGAGGCTGGTTTC
GGTAGAATCAGTAAACTGGGATCTTGTTGGGCTCCAATAGGAGTAGGTCCACATATCCATAGTCACTATATACGAGAATTTCGCTATTGTTATGGAGCTG
TTGATGCCCATACAGGCGAATCATTTTTCTTAATAGCTGGTAGATGTAATACTGAGTGGATGAACGCCTTTTTAGAAGAGCTTTCACAAGCTTATCCAGA
TGATTATCTTTTACTCGTTATGGACAATGCTATATGGCATAAATCAAGTACCTTAAAGATTCCGACTAATATTGGTTTTACCTTTATTCCTCCATACACA
CCAGAGATGAACCCCATTGAACAAGTGTGGAAAGAGATTCGTAAACGTGGATTTAAGAATAAAGCCTTTCAAACTTTGGAAGATGTCATGAATCAACTCC
AAGATGTTATACAAGGATTGGAGAAGGAGGTGATAAAGTCCATCGTTAATCGGAGATGGACTAGAATGCTTTTTGAAAACAGATGAGTA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 507 bp | 168 aa | 39 | 545 | + | No |
Description : First part of the transposase
ORF sequence :
MKSTKEEIQTIKTLLKDSRTAKYHKRLQIVLFRLMGKSYKEIIELLDCNQTTIWRNVKKYEEFGLDSLLQETRGGRNHAYMTVEEKKVFLARHLKAAEAG
EFVTIDALFQAYKKELGRSYTRDAFYQLLKCHGWRNIMPRPEHPKKADAQTIVASKNKISIQEEKKAL
EFVTIDALFQAYKKELGRSYTRDAFYQLLKCHGWRNIMPRPEHPKKADAQTIVASKNKISIQEEKKAL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 663 bp | 217 aa | 424 | 1086 | + | No |
Description : Second part of the transposase
ORF sequence :
SAMVGEILCHVQNILRKQTLKPLSRLKIKSQFKKKRFKTSRRFRKVRLMYQDEAGFGRISKLGSCWAPIGVGPHIHSHYIREFRYCYGAVDAHTGESFFL
IAGRCNTEWMNAFLEELSQAYPDDYLLLVMDNAIWHKSSTLKIPTNIGFTFIPPYTPEMNPIEQVWKEIRKRGFKNKAFQTLEDVMNQLQDVIQGLEKEV
IKSIVNRRWTRMLFENR
IAGRCNTEWMNAFLEELSQAYPDDYLLLVMDNAIWHKSSTLKIPTNIGFTFIPPYTPEMNPIEQVWKEIRKRGFKNKAFQTLEDVMNQLQDVIQGLEKEV
IKSIVNRRWTRMLFENR
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1048 bp | 349 aa | 39 | 1086 | + | Yes |
Chemistry : DDE
ORF sequence :
MKSTKEEIQTIKTLLKDSRTAKYHKRLQIVLFRLMGKSYKEIIELLDCNQTTIWRNVKKYEEFGLDSLLQETRGGRNHAYMTVEEKKVFLARHLKAAEAG
EFVTIDALFQAYKKELGRSYTRDAFYQLLKCHGWRNIMPRPEHPKKADAQTIVASKNKISIQEEKKKRFKTSRRFRKVRLMYQDEAGFGRISKLGSCWAP
IGVGPHIHSHYIREFRYCYGAVDAHTGESFFLIAGRCNTEWMNAFLEELSQAYPDDYLLLVMDNAIWHKSSTLKIPTNIGFTFIPPYTPEMNPIEQVWKE
IRKRGFKNKAFQTLEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFENR
EFVTIDALFQAYKKELGRSYTRDAFYQLLKCHGWRNIMPRPEHPKKADAQTIVASKNKISIQEEKKKRFKTSRRFRKVRLMYQDEAGFGRISKLGSCWAP
IGVGPHIHSHYIREFRYCYGAVDAHTGESFFLIAGRCNTEWMNAFLEELSQAYPDDYLLLVMDNAIWHKSSTLKIPTNIGFTFIPPYTPEMNPIEQVWKE
IRKRGFKNKAFQTLEDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFENR
Blast result :
Comments
ISSpn2 is 49%(ORF1) and 47% (ORF2) aa similar to ISC1048. The third ORf is a fusion in silico.
References
1] Claverys, JP. (2006) Direct submission.