ISVisp1

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
DQ987704 ND Vibrio splendidus
Vibrio splendidus LGP32
DNA section
IS Length : 1267 bp

Ends


IR Length : 21/27

IRL : TGTAGTTGACCCGATCTAATGGACACCTTCAATTGTAATTGGAGGTGTTT
IRR : TGATCTTCACCCGATAAAATGGACACCCTATTATCTTTAACCCGTCGCTA

Insertion site


Left flankDirect repeatRight flankDR Length
TTAAGACAGATTCCCAACGCATG3

DNA sequence

TGTAGTTGACCCGATCTAATGGACACCTTCAATTGTAATTGGAGGTGTTTATGCCAGCTTATAAATCAGGTAAAAAAACTCAACAATATCTTACGGAATT
TAAGGTAACTGCTGTCCGACTTTCGCTTCGAGAAGGAGCTACAGTTAAAAGCGTGGCGCTATCATTAGATATCCACCCTTATATGCTTTCTAAATGGCGA
AAGGACTATAGAGAAGGGGTTATTATGGAAGATAAACGTAAGAAAAGGACTCAGATCCCGAGCCAGAAAACTGAGTCTAGTCGGATTGCAGAGCTTGAAC
GTCAGAACGAACAACTTAAGCTCGAGAATGACTTGCTAAAAAAGTGGCAACGATTCGTTGCCCAAGAAAATCGACAAAATTCCGATTCATAGCCCATTAC
AAAACGCGTTATTCCATTGTCTTGATGTGTCGTTTTCTATCTGTTTCTAAGTCGGGTTACTACGCATGGCTTGATAGAGAGCCAAGCCGCTACGATCAAG
AAGAGCAGGCTTTGAAGAAGCGCATAATTGAAGTGTTTACCCAGAGTCGAGAGACTTACGGTAGTCCACGAGTTCATGCAGAGCTGAGGCGCCAAGGAGT
TTTGGTTAGCCGCAAGCGTGTAGCTCGGATCATGAGAGAGCAAGGGCTAAGAGCGCGAAGTTATCGCATTTACATGAAAATGGCCAAGCTACATCGGTTC
TATCAATCGATTAAGAATATTAAAAAAGACACACCAAAACCAACGGCAGTTAATCAACAATGGTCTGGAGATCTAACGTATATAAAGCAAGGTAAACGTT
GGATGTATCTAGCGGTCGTTATCGATCTTTACTCGCGTAAAATCGTCGGTTGGTCGCTTGGTAGTAAGAAGAGTACACAACTAACGATGAGTTCGTTGAG
AATGGCAATTAGAAATCGAAAGCCACAAGAGCGATTATTATTTCATACCGACAGAGGCTCTGAATATCGAGCCCATGAAGTTCAAGCTTTATTATCAAAA
AATGGAATTGTCCCAAGTATGAATCGTCCAGGTCATTGCACTGATAATGCGGAGGTGGAGTCGTTCTTCCATACGCTCAAAGGCGACATAATTAGGAAAA
ATAGTTTTAAAAGTGAGAAGCAGCTTAGAGATAAACTTGCCGGTTATATCCAGCATTTTTATAACCGTTATAGACTGCACTCAAGTCTTGGATATCGAAC
CCCGCATGAATATGAAGTAGCGACGGGTTAAAGATAATAGGGTGTCCATTTTATCGGGTGAAGATCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
342 bp113 aa51392+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MPAYKSGKKTQQYLTEFKVTAVRLSLREGATVKSVALSLDIHPYMLSKWRKDYREGVIMEDKRKKRTQIPSQKTESSRIAELERQNEQLKLENDLLKKWQ
RFVAQENRQNSDS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
900 bp299 aa3321231+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

VATIRCPRKSTKFRFIAHYKTRYSIVLMCRFLSVSKSGYYAWLDREPSRYDQEEQALKKRIIEVFTQSRETYGSPRVHAELRRQGVLVSRKRVARIMREQ
GLRARSYRIYMKMAKLHRFYQSIKNIKKDTPKPTAVNQQWSGDLTYIKQGKRWMYLAVVIDLYSRKIVGWSLGSKKSTQLTMSSLRMAIRNRKPQERLLF
HTDRGSEYRAHEVQALLSKNGIVPSMNRPGHCTDNAEVESFFHTLKGDIIRKNSFKSEKQLRDKLAGYIQHFYNRYRLHSSLGYRTPHEYEVATG

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1181 bp393 aa511231+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPAYKSGKKTQQYLTEFKVTAVRLSLREGATVKSVALSLDIHPYMLSKWRKDYREGVIMEDKRKKRTQIPSQKTESSRIAELERQNEQLKLENDLLKKVA
TIRCPRKSTKFRFIAHYKTRYSIVLMCRFLSVSKSGYYAWLDREPSRYDQEEQALKKRIIEVFTQSRETYGSPRVHAELRRQGVLVSRKRVARIMREQGL
RARSYRIYMKMAKLHRFYQSIKNIKKDTPKPTAVNQQWSGDLTYIKQGKRWMYLAVVIDLYSRKIVGWSLGSKKSTQLTMSSLRMAIRNRKPQERLLFHT
DRGSEYRAHEVQALLSKNGIVPSMNRPGHCTDNAEVESFFHTLKGDIIRKNSFKSEKQLRDKLAGYIQHFYNRYRLHSSLGYRTPHEYEVATG

 

Blast result :
Comments
ISVisp1 is 52%(ORFA) aa similar to IS1501 and 57%(ORFB) to IS1953. The third ORF is a fusion ORF in silico.
References
1] D. Mazel (2006) Direct submission.