ISMyma1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Mycobacterium marinum | Mycobacterium marinum |
DNA section
IS Length : 1240 bp
Ends
IR Length : 12/13
IRL : TGGCCTGACCCCGATCGGTTGATCCGCGGCTTGTGAGAGTCTTGCGGCCC
IRR : TGGCCGGACCCCGTGAACTGGTCCGCGGCGAACGAGAGTCATTCATCGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTACGACATC | CATC | CTGATTCACA | 4 |
| TGGATTACTG | CACG | CCCTGCCCGT | 4 |
DNA sequence
TGGCCTGACCCCGATCGGTTGATCCGCGGCTTGTGAGAGTCTTGCGGCCCACGTTGTGGGCAGGAAGGCTCGACAAGATCATGGCGACGAGGAAACGGCA
CAGTCCGGAGCAGATTGTGCGCAAGCTGATGACCGCGGACCGGCTGTTGGCCGAGGGCAAGGACACCGCGGCGGTCTGCCGCGAGCTGGGGGTCTCTGAG
GCTACCTACCACCGGTGGCGCAACCAGTTCGGCGGCCTGAAGGCCGAGGACGCTAAACGGCTCAAGGACCTCGAACGCGAGAACGCCGCACTCAAAAGGT
TGTTGGCCGATGCCGAGTTGGAGAAGGACGCGCTGCGGGAGATCGCCCGGGGAAACTTTTAGGCCCGGAGCGCCGGCGGGCGGCCGTTCGTCATCTCCAG
CGCGTGCTGGGGGTCAGCGAGCGGTTTGCCTGCCGCGTCACCGGGCAGCACCGCGCCACCCAACGCCACGAACCGGTTGCGGGCACGCCGGCCGATCCGG
ACGCCGCTCTGAGGGAGTGGCTACGCGACTACGCCAACAAGCATCCTCGTCGCGGGTTCCGACCGGCCTACCACGATGCCCGCGGCGAAGGCTGGGAAGT
GAACCATAAGAAGGTGCAACGGCTTTGGCGTGAGGAAGGACTGCGGGTCCCGCAGCGTCGCAAGCGCAAACGGCACGGCACCTCCACCGCCCCGCCAGAG
GTAGTCGCCGATGCACCCGATCGGGTGTGGGCGGTGGACTTCCAGTTCGACGTGACCACCGATGGGCGGCCGATCAAGATCGTGTCGATCATCGACGAGC
ACACCCGCGAATGCCTCGGCGGTATGGTCGAGCGCAGCATCACCGGTGATCACCTCACCGAGGAGTTGGGCCGCATAGCCGCCGGGCGCGGGGCCTTACC
CGCGGTGCTGCGGTGTGACAACGGACCCGAATTGGCTTGCAGCGCAATGGCAGACTGGGCCGAGGGCCAGGTCGGACTCCACTTCATCCCGCCCGGCGAG
CCGTGGCGCAACGGCTATGTCGAGTCGTTCAACTCCCGCATCCGCGACGAGTGCCTCAACATCAATAGCTTCTGGTCGCTGGCCCAGGCCCGTGTCGTCA
TCGGCGACTGGAAGCACGAGTACAACCATCATCGGCGGCATTCATCGCTGGGCTACCTACCGCCTGCCCGCTATGCTGCCGCCTGTACCCACCGATGAAT
GACTCTCGTTCGCCGCGGACCAGTTCACGGGGTCCGGCCA
CAGTCCGGAGCAGATTGTGCGCAAGCTGATGACCGCGGACCGGCTGTTGGCCGAGGGCAAGGACACCGCGGCGGTCTGCCGCGAGCTGGGGGTCTCTGAG
GCTACCTACCACCGGTGGCGCAACCAGTTCGGCGGCCTGAAGGCCGAGGACGCTAAACGGCTCAAGGACCTCGAACGCGAGAACGCCGCACTCAAAAGGT
TGTTGGCCGATGCCGAGTTGGAGAAGGACGCGCTGCGGGAGATCGCCCGGGGAAACTTTTAGGCCCGGAGCGCCGGCGGGCGGCCGTTCGTCATCTCCAG
CGCGTGCTGGGGGTCAGCGAGCGGTTTGCCTGCCGCGTCACCGGGCAGCACCGCGCCACCCAACGCCACGAACCGGTTGCGGGCACGCCGGCCGATCCGG
ACGCCGCTCTGAGGGAGTGGCTACGCGACTACGCCAACAAGCATCCTCGTCGCGGGTTCCGACCGGCCTACCACGATGCCCGCGGCGAAGGCTGGGAAGT
GAACCATAAGAAGGTGCAACGGCTTTGGCGTGAGGAAGGACTGCGGGTCCCGCAGCGTCGCAAGCGCAAACGGCACGGCACCTCCACCGCCCCGCCAGAG
GTAGTCGCCGATGCACCCGATCGGGTGTGGGCGGTGGACTTCCAGTTCGACGTGACCACCGATGGGCGGCCGATCAAGATCGTGTCGATCATCGACGAGC
ACACCCGCGAATGCCTCGGCGGTATGGTCGAGCGCAGCATCACCGGTGATCACCTCACCGAGGAGTTGGGCCGCATAGCCGCCGGGCGCGGGGCCTTACC
CGCGGTGCTGCGGTGTGACAACGGACCCGAATTGGCTTGCAGCGCAATGGCAGACTGGGCCGAGGGCCAGGTCGGACTCCACTTCATCCCGCCCGGCGAG
CCGTGGCGCAACGGCTATGTCGAGTCGTTCAACTCCCGCATCCGCGACGAGTGCCTCAACATCAATAGCTTCTGGTCGCTGGCCCAGGCCCGTGTCGTCA
TCGGCGACTGGAAGCACGAGTACAACCATCATCGGCGGCATTCATCGCTGGGCTACCTACCGCCTGCCCGCTATGCTGCCGCCTGTACCCACCGATGAAT
GACTCTCGTTCGCCGCGGACCAGTTCACGGGGTCCGGCCA
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 282 bp | 93 aa | 81 | 362 | + | No |
Description : First part of the transposase
ORF sequence :
MATRKRHSPEQIVRKLMTADRLLAEGKDTAAVCRELGVSEATYHRWRNQFGGLKAEDAKRLKDLERENAALKRLLADAELEKDALREIARGNF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 943 bp | 313 aa | 256 | 1198 | + | No |
Description : Second part of the transposase
ORF sequence :
TAQGPRTRERRTQKVVGRCRVGEGRAAGDRPGKLLGPERRRAAVRHLQRVLGVSERFACRVTGQHRATQRHEPVAGTPADPDAALREWLRDYANKHPRRG
FRPAYHDARGEGWEVNHKKVQRLWREEGLRVPQRRKRKRHGTSTAPPEVVADAPDRVWAVDFQFDVTTDGRPIKIVSIIDEHTRECLGGMVERSITGDHL
TEELGRIAAGRGALPAVLRCDNGPELACSAMADWAEGQVGLHFIPPGEPWRNGYVESFNSRIRDECLNINSFWSLAQARVVIGDWKHEYNHHRRHSSLGY
LPPARYAAACTHR
FRPAYHDARGEGWEVNHKKVQRLWREEGLRVPQRRKRKRHGTSTAPPEVVADAPDRVWAVDFQFDVTTDGRPIKIVSIIDEHTRECLGGMVERSITGDHL
TEELGRIAAGRGALPAVLRCDNGPELACSAMADWAEGQVGLHFIPPGEPWRNGYVESFNSRIRDECLNINSFWSLAQARVVIGDWKHEYNHHRRHSSLGY
LPPARYAAACTHR
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1118 bp | 372 aa | 81 | 1198 | + | Yes |
Chemistry : DDE
ORF sequence :
MATRKRHSPEQIVRKLMTADRLLAEGKDTAAVCRELGVSEATYHRWRNQFGGLKAEDAKRLKDLERENAALKRLLADAELEKDALREIARGKLLGPERRR
AAVRHLQRVLGVSERFACRVTGQHRATQRHEPVAGTPADPDAALREWLRDYANKHPRRGFRPAYHDARGEGWEVNHKKVQRLWREEGLRVPQRRKRKRHG
TSTAPPEVVADAPDRVWAVDFQFDVTTDGRPIKIVSIIDEHTRECLGGMVERSITGDHLTEELGRIAAGRGALPAVLRCDNGPELACSAMADWAEGQVGL
HFIPPGEPWRNGYVESFNSRIRDECLNINSFWSLAQARVVIGDWKHEYNHHRRHSSLGYLPPARYAAACTHR
AAVRHLQRVLGVSERFACRVTGQHRATQRHEPVAGTPADPDAALREWLRDYANKHPRRGFRPAYHDARGEGWEVNHKKVQRLWREEGLRVPQRRKRKRHG
TSTAPPEVVADAPDRVWAVDFQFDVTTDGRPIKIVSIIDEHTRECLGGMVERSITGDHLTEELGRIAAGRGALPAVLRCDNGPELACSAMADWAEGQVGL
HFIPPGEPWRNGYVESFNSRIRDECLNINSFWSLAQARVVIGDWKHEYNHHRRHSSLGYLPPARYAAACTHR
Blast result :
Comments
ISMyma1 is 79% aa similar to ISPfr10.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Stinear Tim. (2006) Direct submission.