ISBcen10
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Burkholderia cenocepacia | Burkholderia cenocepacia J2315 |
DNA section
IS Length : 1447 bp
Ends
IR Length : 18/24
IRL : TGAGCTGCACCCCAAAAGTTGGACTAGATCCAACCTTTGGGGTGTTTTTC
IRR : TGAACTGACCCCACGAAGTTGGACGGTTAAAAGCTAAGCGGCCAAGGGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGGCCGCTCG | TTCG | AGCGGCCGTT | 4 |
| AACCGGCCGC | ATC | AGCGGCCGGT | 3 |
| CGGCCGCTCG | ATCA | CGAGCGGCCG | 4 |
| AACCGGCCAC | GTC | GCGCGCCGGC | 3 |
DNA sequence
TGAGCTGCACCCCAAAAGTTGGACTAGATCCAACCTTTGGGGTGTTTTTCATGACGAAGTACGACGAACGTTTTCGGCGTCGAGTGGTTCAGGCGTACCT
GGCTGGAGAGGCCGGCACCAAGACACTTGCTCACCGTTACGGAGTGGGCAGGACAATGGTGCGACGTTGGGTGGCGAGCTATCGCGAGCATGGTGAACAG
GGGCTGCGCAAGAAGCACAGCCACTACGACGCGCGATTCAAGTTGTCAGTCCTTCAGCGGATGCGGCGCGATGAACTGTCGTATGCGCAGGTGGCAGCGC
TGTTCGGCATCCGCAATGAACGAAGCATCCCGATCTGGGAGCGTCTGTATCATGAGGGCGGTATAGATGCGTTGGCGCCTCGTCGCCGAGGGCGCCCTCC
GAAAATGACCACCTCACCACCACCGAAATCCCCCGACGATACCGTGCAGAAAGAGCCGAGCCGCGAGGAACTGCTCAAGGAAATCGTGTATTTGCGCGCG
GAGGTGGCATACCTAAAAAAGCTCGATGCGCTGCTGCAATCCAAGAAGCAGGCAGCGCCCAGGAAAAAGCGCAAATAGTGCGTGAGCTCAGGCAGTGTCA
TCCGGTAGCTGCGCTCCTGAAGGCGGCAGGCCTGGCGCGCAGCACGTTCTATTACCAGCTCAAGGCATTGGCTGCCGGCGATCGGTATGCGGATCTCAAA
GCGAAGATCCAGACCGTATACGACCACCACAAGGGCCGTTACGGCTATCGGCGAATCACGGCGGCGATCCGGCAGACCGGAGATGCGATCAACCACAAGA
CCGTGCAACGGTTGATGGGACAGCTGCAATTGAAATCTCTGGTACGCCCAAAGAGATATCGTTCCTGGCGAGGCGAAGTCGGCCGCGTTGCCCCGAATCT
ACTGCGGCGTCAGTTTGATGCTGAACGCCCGAATCAGAAGTGGGTGACCGACGTGACCGAATTCAATGTAGAGGGCCAAAAGCTCTATCTATCGCCGGTG
ATGGACTTGTACAACGGGGAGATCGTCGCTTACCAAATGGATGAGCGCCCGAGCTTCGAGTTGGTCAGCTGCATGTTGAAGAAGGCGTTGGCCAAGCTCG
ATGATGAAGGACGGCCGTTACTGCATTCGGATCAAGGCTGGCAGTATCAGATGCCCGCCTACCGTCGGCTGTTGAAGCAGCATGCGCTCACCCAGAGCAT
GTCGCGCAGAGGCAACTGCCTTGATAACGCAGCCATGGAGAGCTTCTTCGGCACGCTGAAATCCGAGTGCTATCGGCTGACGCGCTTTGCGAGCGTGGAG
CAGTTGCGAGATGCGTTGAGCCGCTACATCCACTACTACAACCACGAACGCATCAAGCTGAAATTAAATGGGCTGAGTCCCGTGCAATACAGGACTCAGC
CCTTGGCCGCTTAGCTTTTAACCGTCCAACTTCGTGGGGTCAGTTCA
GGCTGGAGAGGCCGGCACCAAGACACTTGCTCACCGTTACGGAGTGGGCAGGACAATGGTGCGACGTTGGGTGGCGAGCTATCGCGAGCATGGTGAACAG
GGGCTGCGCAAGAAGCACAGCCACTACGACGCGCGATTCAAGTTGTCAGTCCTTCAGCGGATGCGGCGCGATGAACTGTCGTATGCGCAGGTGGCAGCGC
TGTTCGGCATCCGCAATGAACGAAGCATCCCGATCTGGGAGCGTCTGTATCATGAGGGCGGTATAGATGCGTTGGCGCCTCGTCGCCGAGGGCGCCCTCC
GAAAATGACCACCTCACCACCACCGAAATCCCCCGACGATACCGTGCAGAAAGAGCCGAGCCGCGAGGAACTGCTCAAGGAAATCGTGTATTTGCGCGCG
GAGGTGGCATACCTAAAAAAGCTCGATGCGCTGCTGCAATCCAAGAAGCAGGCAGCGCCCAGGAAAAAGCGCAAATAGTGCGTGAGCTCAGGCAGTGTCA
TCCGGTAGCTGCGCTCCTGAAGGCGGCAGGCCTGGCGCGCAGCACGTTCTATTACCAGCTCAAGGCATTGGCTGCCGGCGATCGGTATGCGGATCTCAAA
GCGAAGATCCAGACCGTATACGACCACCACAAGGGCCGTTACGGCTATCGGCGAATCACGGCGGCGATCCGGCAGACCGGAGATGCGATCAACCACAAGA
CCGTGCAACGGTTGATGGGACAGCTGCAATTGAAATCTCTGGTACGCCCAAAGAGATATCGTTCCTGGCGAGGCGAAGTCGGCCGCGTTGCCCCGAATCT
ACTGCGGCGTCAGTTTGATGCTGAACGCCCGAATCAGAAGTGGGTGACCGACGTGACCGAATTCAATGTAGAGGGCCAAAAGCTCTATCTATCGCCGGTG
ATGGACTTGTACAACGGGGAGATCGTCGCTTACCAAATGGATGAGCGCCCGAGCTTCGAGTTGGTCAGCTGCATGTTGAAGAAGGCGTTGGCCAAGCTCG
ATGATGAAGGACGGCCGTTACTGCATTCGGATCAAGGCTGGCAGTATCAGATGCCCGCCTACCGTCGGCTGTTGAAGCAGCATGCGCTCACCCAGAGCAT
GTCGCGCAGAGGCAACTGCCTTGATAACGCAGCCATGGAGAGCTTCTTCGGCACGCTGAAATCCGAGTGCTATCGGCTGACGCGCTTTGCGAGCGTGGAG
CAGTTGCGAGATGCGTTGAGCCGCTACATCCACTACTACAACCACGAACGCATCAAGCTGAAATTAAATGGGCTGAGTCCCGTGCAATACAGGACTCAGC
CCTTGGCCGCTTAGCTTTTAACCGTCCAACTTCGTGGGGTCAGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 525 bp | 175 aa | 51 | 575 | + | No |
Description : First part of the transposase
ORF sequence :
MTKYDERFRRRVVQAYLAGEAGTKTLAHRYGVGRTMVRRWVASYREHGEQGLRKKHSHYDARFKLSVLQRMRRDELSYAQVAALFGIRNERSIPIWERLY
HEGGIDALAPRRRGRPPKMTTSPPPKSPDDTVQKEPSREELLKEIVYLRAEVAYLKKLDALLQSKKQAAPRKKRK
HEGGIDALAPRRRGRPPKMTTSPPPKSPDDTVQKEPSREELLKEIVYLRAEVAYLKKLDALLQSKKQAAPRKKRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1056 bp | 352 aa | 356 | 1411 | + | No |
Description : Second part of the transposase
ORF sequence :
GRYRCVGASSPRAPSENDHLTTTEIPRRYRAERAEPRGTAQGNRVFARGGGIPKKARCAAAIQEAGSAQEKAQIVRELRQCHPVAALLKAAGLARSTFYY
QLKALAAGDRYADLKAKIQTVYDHHKGRYGYRRITAAIRQTGDAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLRRQFDAERPNQKWVTDV
TEFNVEGQKLYLSPVMDLYNGEIVAYQMDERPSFELVSCMLKKALAKLDDEGRPLLHSDQGWQYQMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFFGT
LKSECYRLTRFASVEQLRDALSRYIHYYNHERIKLKLNGLSPVQYRTQPLAA
QLKALAAGDRYADLKAKIQTVYDHHKGRYGYRRITAAIRQTGDAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLRRQFDAERPNQKWVTDV
TEFNVEGQKLYLSPVMDLYNGEIVAYQMDERPSFELVSCMLKKALAKLDDEGRPLLHSDQGWQYQMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFFGT
LKSECYRLTRFASVEQLRDALSRYIHYYNHERIKLKLNGLSPVQYRTQPLAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1361 bp | 454 aa | 51 | 1411 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKYDERFRRRVVQAYLAGEAGTKTLAHRYGVGRTMVRRWVASYREHGEQGLRKKHSHYDARFKLSVLQRMRRDELSYAQVAALFGIRNERSIPIWERLY
HEGGIDALAPRRRGRPPKMTTSPPPKSPDDTVQKEPSREELLKEIVYLRAEVAYLKKARCAAAIQEAGSAQEKAQIVRELRQCHPVAALLKAAGLARSTF
YYQLKALAAGDRYADLKAKIQTVYDHHKGRYGYRRITAAIRQTGDAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLRRQFDAERPNQKWVT
DVTEFNVEGQKLYLSPVMDLYNGEIVAYQMDERPSFELVSCMLKKALAKLDDEGRPLLHSDQGWQYQMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFF
GTLKSECYRLTRFASVEQLRDALSRYIHYYNHERIKLKLNGLSPVQYRTQPLAA
HEGGIDALAPRRRGRPPKMTTSPPPKSPDDTVQKEPSREELLKEIVYLRAEVAYLKKARCAAAIQEAGSAQEKAQIVRELRQCHPVAALLKAAGLARSTF
YYQLKALAAGDRYADLKAKIQTVYDHHKGRYGYRRITAAIRQTGDAINHKTVQRLMGQLQLKSLVRPKRYRSWRGEVGRVAPNLLRRQFDAERPNQKWVT
DVTEFNVEGQKLYLSPVMDLYNGEIVAYQMDERPSFELVSCMLKKALAKLDDEGRPLLHSDQGWQYQMPAYRRLLKQHALTQSMSRRGNCLDNAAMESFF
GTLKSECYRLTRFASVEQLRDALSRYIHYYNHERIKLKLNGLSPVQYRTQPLAA
Blast result :
Comments
There are 4 copies in the genome of Burkholderia cenocepacia strain J2315.
ISBcen10 is 66% (ORF1) aa similar to IS1221A and 81% (ORF2) to ISRso11.
ISBcen10 is 66% (ORF1) aa similar to IS1221A and 81% (ORF2) to ISRso11.
References
1] The Wellcome Trust Sanger Institute. http://www.sanger.ac.uk
2] Seth-Smith, H. (2006) Direct submission
2] Seth-Smith, H. (2006) Direct submission