ISPpu20

  • Family IS1182
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AB238971 ND Pseudomonas putida
Pseudomonas putida MT53 plasmid pWW53
DNA section
IS Length : 1598 bp

Ends


IR Length : 16

IRL : CAGGCTGTGTGAAAACCTAGCAATCTGCTGACCGGTTAAAGAAAATGCCC
IRR : CAGACCGTGTGAAAACACAATCTGCCCCGGCTAAAAGCAAATGCcCCGAC

Insertion site


Left flankDirect repeatRight flankDR Length
AAAACCCGCCATTAAGGCGGGTTTT5

DNA sequence

CAGGCTGTGTGAAAACCTAGCAATCTGCTGACCGGTTAAAGAAAATGCCCGTATCGCAAGATACGGGCATTTTCCTTATGCGCTATATCCAAGGTGAAAA
CCGCTCTCAGAGCGCGCTGTTTCCACTCTCGCTGGACGAACTCATTCCCGAGGATCACCTGGTTCGGGTCATCGAAGCCTACATTTCCCTTCTGGACTTT
GAGCAGTTGGGCTTCGACAAAGCCAGACCCAAGGCCACGGGACGCCCTTCGTACGACCCTGCCGATTTGCTCAAACTCTATCTTTACGGCTATTTCCAGC
GGATTCGCTCCTCGCGACGCCTTGAGGCTGAGTGTCAGCGCAACGTcGAAGTCATGTGGCTATTGGGCCGACTGGCACCTGATTTCAAAACCATTGCTGA
TTTTCGACGCGACAACAGCGCTGCGTTTACCGCGACCTGTCGTGCCTTCGTTCGTTTCTGCCGAGAGGCTGGTCTGATTGCCGGCGAGTTGGTGGCCATC
GATGGCAGCAAGTTTCAGGCGGTGGCTTCCAGGCGCAGACACCTGACGCCGAAAAAGCTCCAGCATCGGGAAGCGGCGCTCGACAAGAAAATTGCGCGTT
ATCTGGCGCAACTCGATGAAGCGGATCGTGATGAAGAGGGCGAGTCGATCGACCGCTCAGCCGTGCAGGCGGCACTGGCGCAGTTACAGGTAAAAAAGAC
CAATAACCAGACCTGCCAAGTGCTGATGCAAGCTCAAGGTCTGGTTCAGCACATTGTGGGCGAGCCTGATGCGCAGAAAATGCGAACGGCATCCGGCCCG
CTCGTGGCCTACAACATTCAGAGTGCGGTGGACGCCAAACATGGCCTGATTCTGCACCACGAAGTCACTCAGGAAGGCACTGACAATCGTCAACTTGAAC
CGATGGCCAAGGCGGCCAAAGAGGCGCTCAAGCAATCCGAACTTTGCGTCACCGCCGATGCAGGTTACTCCAATGGCGCCCAGTTTCAGGCCTGTGAAGA
CGCCGGCATCACGGCGTTTGTGCCGCCCAATCGAGCCATCAACACCACCGGTGGCGACGAGCAGTACTTTGATCGAACCGAATTCAGCTATGACTCGGAC
AGCGATCAATATCAGTGCCCCGCCGGCAACCAACTGAGCCTCAAACAACTGAGCAAGGGCGACCGGATCTATCAGGCGGCTATCAGCGACTGCCGAAGTT
GTCCGCTCAAAAGCCGCTGCACCAAGGCCGAACGCCGGTACCTGACGCGCCATGCCCACGAAGCGGCATTTGAGCGAATGGAGCGGCGAATGAAGGCCCA
CCCTGAAATGATGGTCAATCGCCGATCCATCGTCGAGCATCCCTTCGGCAATCTGAAGCAATGGATCTTCGGCAACGGCCGCTTCTTGTTGCGCCAGTTG
CAGGGCGCTCGTGCAGAAATGGCGCTTGCGGTACAGGCCTACAACTTGAAAAGGGTTATCCAGGTGCTGGGAGCACAGAAGCTGATGGCGCTGATGGGCT
GAGAAGCCCTTTATTTTTCAGATTTCAGAAAAGCAAATGCCCCGACAAGTCGGgGCATTTGCTTTTAGCCGGGGCAGATTGTGTTTTCACACGGTCTG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1458 bp485 aa451502+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPVSQDTGIFLMRYIQGENRSQSALFPLSLDELIPEDHLVRVIEAYISLLDFEQLGFDKARPKATGRPSYDPADLLKLYLYGYFQRIRSSRRLEAECQRN
VEVMWLLGRLAPDFKTIADFRRDNSAAFTATCRAFVRFCREAGLIAGELVAIDGSKFQAVASRRRHLTPKKLQHREAALDKKIARYLAQLDEADRDEEGE
SIDRSAVQAALAQLQVKKTNNQTCQVLMQAQGLVQHIVGEPDAQKMRTASGPLVAYNIQSAVDAKHGLILHHEVTQEGTDNRQLEPMAKAAKEALKQSEL
CVTADAGYSNGAQFQACEDAGITAFVPPNRAINTTGGDEQYFDRTEFSYDSDSDQYQCPAGNQLSLKQLSKGDRIYQAAISDCRSCPLKSRCTKAERRYL
TRHAHEAAFERMERRMKAHPEMMVNRRSIVEHPFGNLKQWIFGNGRFLLRQLQGARAEMALAVQAYNLKRVIQVLGAQKLMALMG

 

Blast result :
Comments
ISPpu20 is located on pWW53. ORF1 exhibits 71% amino acid identity with ISPsy6 ORF1
References
1] Yano, H., Tsuda, M. (2006) Direct Submission
2] Yano,H., Garruto,C.E., Sota,M., Ohtsubo,Y., Nagata,Y., Zylstra,G.J., Williams,P.A. and Tsuda,M.(2007) J. Mol. Biol. 369 (1), 11-26