ISBcen19

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Burkholderia cenocepacia
Burkholderia cenocepacia J2315
DNA section
IS Length : 2450 bp

Ends


IR Length : 19

IRL : GTAAGCGGCCAGCCAACCCGTCTTGAGTTTGGCCGAGGCGCTTGGGAGTA
IRR : GTAAGCGGCCAGCCAACCCATCTTGACGAGCGAGTTTAGGCGGCGGCCGA

Insertion site


Left flankDirect repeatRight flankDR Length
GCGTTCGTGTCGACGCCCAC0
TCACGTCAACACGTCAACAA0

DNA sequence

GTAAGCGGCCAGCCAACCCGTCTTGAGTTTGGCCGAGGCGCTTGGGAGTATCGTGTCCACTTAACGAATAAGCGGACACGTGAACACTATCGAATCGGAA
TCACTCCCGGAGCGTCGTCGTCGGCAACGCTATAGTGAGGAGTTCAAGGCGCAGGTGGTAGCGGCCTGCCAGGGAATCGGCGTATCGGTTGCGGCGGTCG
CTCTGGAACACAGGCTCAACGCGAATCTGCTGCGGCGCTGGATCGATCAGGCGGAAGGCCGACTTCCCAAGAGACCGCTGGGCCGTCCGCCGGCCACGCC
ACCGTCGCTGCCAGCCTTTGTGCCGGTGGCATTGGGAGCGCCGAGTCCGCATTCGCCGGAGATCCGCATTGAGATTCGCCGAGGAGATCAGTCGATCACG
GTGAGTTGGCCGGTATCCGAGGCAACCCAGTGTGCCGTCTGGCTGCGCGAGTGGCTGCGGTGATTCGTGTCGACGAGATCTGGCTGGCGGTCGATCCACT
GGACATGCGGGCCGGTTTCGACACGGCCCTGGCACGTGTGGTCAAGGTGTTCGGCGCCGCGCACCCACACCATGCCTATCTGTTCGCCAATCGGCGCGCC
ACCCGCATGAAGGTCCTGATTCACGACGGGATCGGCATCTGGCTGGCCGCGCGACGCCTGAATCAAGGGCAGTTCGCGTGGCCGCACATCGGCAGCGAAC
CGAAGCAGCGTGTCTTGACGCACGAACAACTGGCCGGCCTGGTGCTGGGCTTGCCCTGGCAACGCATCGGCGAGGATGGCGTGATCCGTGTCGTCTAAAT
CCGGCGGGGAGGTAAACAGTCACGCTTCCTCCGTCGGCAGGGTTCTGGCAGACTGACCCGCATGAACCCCGCCGACCTCGACGCACTCAACCCGGAACAG
TTGCGCGCCCTTGCCGCGCAACTGATTGCCGAGGTCAAGGAGAAGGAGCGGGAAGCGAGTGAACGAGACAGGGAGCTACGCTTCCGGCAGACCCGTATCG
ATCAACTCACCCATGAGGTCTCGATCCTCAAGCGCCAGCAGTTCGGCCGGCGCAGCGAACAGCTCAACAGCGAGCAGATGAACCTGCTCGACGAGGCCAT
CGATGGCGACTTGGTTGCCATCGAGATGGAACTCGAGCAACTCGAGCCGACGCGAGCTGAACGGCAACGCGAGCAACCGAAACGGGCACTGCTGCCGCCG
CAACTGCCACGCACCGACGTCCATCACGAACCGGACAGCACAACGTGCCAGTGCGGTTGCGAACGCATACGGATCGGCGAGGACGTGAGCGAGAAGCTGG
ACTACACGCCGGGCGTGTTTACGGTGGAACGGCACATCCGCGGCAAGTGGGTGTGCAAGGCGTGCGAGACGCTGATCCAGGCGCCGGTTCCACCGCATGT
GATCGACAAGGGCATGCCCACCGCCGGGTTGCTCGCGCAGGTACTGGTCGCTAAGTACGGCGACCACTTGCCCCTGTATCGCCAGGAACAGATCTTTGGT
CGTGCGGGACTGGCCATCCCGAGATCCACGCTGGGTGCCTGGGTGGGCCGGTGTGGCGTGCAGTTGCAGCCGTTGGTCGACGCACTCGGCCAGGCCATCC
GGCAACAGGCGGTGTTGCACGCCGACGAGACGCCGGTGCAAATGCTCAGTCCCGGCAAGGGTAAAACGCACCGGGCATACCTGTGGGCGTATACGTCGAC
TCAGTTCAGCGAACTGCGCGCGGTGGTCTACGACTTCGCCGAGAGCCGCGCCGGTGCGCACGCTCGCACGTTCCTGAAGGGCTGGCACGGCAAGCTGGTA
TGCGACGACTACAGCGGGTACAAAGCCTCGTTCCAGCACGGCATCACCGAGATCGGATGCGCGGCCCATGCCCGACGCAAGTTCTTTGAATTGCACGCCA
ACCACAGCAGCCAGGTTGCGGGGCAGGCCCTGCCGTTCTTTACCGCGCTCTACGACATCGAACGCGAAGCCGCAGCACTGGATGCCGACGAACGGCAGCG
GCTTAGACAGCGTCGGGCCAAGCCGGTGTGCGACGCCCTCTATGAATGGCTGATGGCGCAGCGCAAACTCGTTGTGGAGGGCTCGGCGATTGCGAAGGCG
CTGGACTACAGCCTGAAACGATGGGATGCACTCACGCGCTATCTCGATGACGGGAATGTGCCGATCGACAACAACTGGGTCGAAAACCAGATCCGTCCGT
GGGCAGTCGGGCGGTCCAATTGGTTGTTCGCCGGATCACTGCGTGCGGGTCAGCGCGCGGCCGCCATCATGAGCTTGATCCGCTCGGCACAACTGAACGG
GCTCGAGCCTCTCGCCTATCTGAAGGACGTGCTGACGCGCTTGCCGACCCACAAGGCCAACGACATCGACGCGCTGCTGCCGCATCGCTGGCAGCCACTC
TCGGCCGCCGCCTAAACTCGCTCGTCAAGATGGGTTGGCTGGCCGCTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
384 bp127 aa80463+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MNTIESESLPERRRRQRYSEEFKAQVVAACQGIGVSVAAVALEHRLNANLLRRWIDQAEGRLPKRPLGRPPATPPSLPAFVPVALGAPSPHSPEIRIEIR
RGDQSITVSWPVSEATQCAVWLREWLR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
339 bp112 aa460798+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIRVDEIWLAVDPLDMRAGFDTALARVVKVFGAAHPHHAYLFANRRATRMKVLIHDGIGIWLAARRLNQGQFAWPHIGSEPKQRVLTHEQLAGLVLGLPW
QRIGEDGVIRVV

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1554 bp517 aa8622415+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNPADLDALNPEQLRALAAQLIAEVKEKEREASERDRELRFRQTRIDQLTHEVSILKRQQFGRRSEQLNSEQMNLLDEAIDGDLVAIEMELEQLEPTRAE
RQREQPKRALLPPQLPRTDVHHEPDSTTCQCGCERIRIGEDVSEKLDYTPGVFTVERHIRGKWVCKACETLIQAPVPPHVIDKGMPTAGLLAQVLVAKYG
DHLPLYRQEQIFGRAGLAIPRSTLGAWVGRCGVQLQPLVDALGQAIRQQAVLHADETPVQMLSPGKGKTHRAYLWAYTSTQFSELRAVVYDFAESRAGAH
ARTFLKGWHGKLVCDDYSGYKASFQHGITEIGCAAHARRKFFELHANHSSQVAGQALPFFTALYDIEREAAALDADERQRLRQRRAKPVCDALYEWLMAQ
RKLVVEGSAIAKALDYSLKRWDALTRYLDDGNVPIDNNWVENQIRPWAVGRSNWLFAGSLRAGQRAAAIMSLIRSAQLNGLEPLAYLKDVLTRLPTHKAN
DIDALLPHRWQPLSAAA

 

Blast result :
Comments
There are 2 copies on J2315 chromosome.
ISBcen19 is 54% (ORF1), 79% (ORf2) and 79%(ORF3) aa similar to ISPpu14.
References
1] The Wellcome Trust Sanger Institute. http://www.sanger.ac.uk
2] Seth-Smith Helena (2006) Direct submission