ISBcen20

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform IS402Synonym(s) ISBmu15
Accession numberTranspositionOriginHost
ND Burkholderia cenocepacia
Burkholderia cenocepacia J2315
Burkholderia multivorans ATCC 17616
DNA section
IS Length : 914 bp

Ends


IR Length : 17

IRL : GAGACGGTTTCAAAAAGGCCCGGTGGGGCTGTTAACTTGCGCGGCGAGCT
IRR : GAGACGGTTTCATAAAGACTGGTCGGCCCGCCGAAGGGTATTCCAGCAGA

Insertion site


Left flankDirect repeatRight flankDR Length
GACCGAAAGTTTAGATCGAAACG3
AGCAAAAGACAAAGGCAACAAAG3
GCTTGGTGATTAAGACTGACGTA3
CTTCCGGTCCTTAAAAGATCTGA3
CTTGAGAAGCTTAATTTGTCATA3
ATGATATGACTTACGTTCTTCAC3
GCTACGAGCCTAAGAAGCCGCGA3
GTCAATGTACTTAAAAGGAAAAC3
GGTGTCCATCTAAGGTTGTGTGG3

DNA sequence

GAGACGGTTTCAAAAAGGCCCGGTGGGGCTGTTAACTTGCGCGGCGAGCTGTTAACCTCAGGCATCGGAACAACTGAGACCGAGGAGATGGCCAAGCCGA
TCATTGACGACGAACTGTGGACATTGATCGAGCCGTTATTGCCGCCACCCAAGCCGCGGCGCGAGAAAAACCCAGGCCGCCTGCCTGTTTCGAATCGCGC
CGCGCTGACCGGCATCCTGTTCGTTCTCAAGACCGGACTGCGCTGGCGCGACCTGCCCGCCGAGATGGGCTGCGGCTCGGGCGTGACTTGCTGGCGACGG
CTACGCGATTGGCAGGCTGCGGGCGTATGGGATCGCCTGCACGAATTGCTGCTTGCGAAGCTGCGAGCAGCAGACCAAATCGATTTCTCGCGAGCCGCAG
TCGATTCCTCATCGATTCGCGCCGTTGGGGCGGGCCAAAAACTGGGCCAAACCCCACCGATCGCGCGCGACCAGGTTCTAAGCACCACATCGTCACCGAC
GCCAATGGCACGCCGCTCGCGGCGATCCTGACCGGCGCGAACGTTCACGATGTCACGCAGCTGCTGCCGCTGATCGATGCGATTCCGCCAATTCGTGGGT
TGCGCGGCCACCCACTGCAGAGACCGCGCGTGGTCTACGCCGATCGCGGTTACGACTCTGAGCGGCATCGACGCGCGTTGCGCGATCGCGGTATCGAGCC
GGTTATCGCCAAGCGCCGCACCGAACATGGCAGCGGCCTTGGAAAATATCGCTGGGTCGTTGAACGCACGCATGCCTGGCTGCATCACTTCCGTCGTCTC
CGCATTCGTTTCGAGCGCCGTGCAGACATTCACGGCGCGTTCCTCAAACTCGGTTGCTGCCTGATCTGCTGGAATACCCTTCGGCGGGCCGACCAGTCTT
TATGAAACCGTCTC
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
442 bp147 aa88529+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MAKPIIDDELWTLIEPLLPPPKPRREKNPGRLPVSNRAALTGILFVLKTGLRWRDLPAEMGCGSGVTCWRRLRDWQAAGVWDRLHELLLAKLRAADQIDF
SRAAVDSSSIRAVGAGQKLGQTPPIARDQVLSTTSSPTPMARRSRRS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
462 bp154 aa441902+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TGPNPTDRARPGSKHHIVTDANGTPLAAILTGANVHDVTQLLPLIDAIPPIRGLRGHPLQRPRVVYADRGYDSERHRRALRDRGIEPVIAKRRTEHGSGL
GKYRWVVERTHAWLHHFRRLRIRFERRADIHGAFLKLGCCLICWNTLRRADQSL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
815 bp273 aa88902+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAKPIIDDELWTLIEPLLPPPKPRREKNPGRLPVSNRAALTGILFVLKTGLRWRDLPAEMGCGSGVTCWRRLRDWQAAGVWDRLHELLLAKLRAADQIDF
SRAAVDSSSIRAVGAGQKTGPNPTDRARPGSKHHIVTDANGTPLAAILTGANVHDVTQLLPLIDAIPPIRGLRGHPLQRPRVVYADRGYDSERHRRALRD
RGIEPVIAKRRTEHGSGLGKYRWVVERTHAWLHHFRRLRIRFERRADIHGAFLKLGCCLICWNTLRRADQSL

 

Blast result :
Comments
9 copies on J2315 chromosome. Transposase annotated involving a frameshift, as seen in IS5 family members. The ORF3 is a fusion ORF.
References