ISPg8
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_002950 | ND | Porphyromonas gingivalis | Porphyromonas gingivalis W83 |
DNA section
IS Length : 1338 bp
Ends
IR Length : 12/12
IRL : GAGACCTTTGCACGGCGATTGGCGTATATTTTGTTTGTTAATTCATTGTA
IRR : GAGACCTTTGCAAAATAAGGAGATGGAGGGAAGAGGAGTTCTTGGCAAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TACCACTAAT | ATAATCAGAA | 0 | |
| TATGTATAAT | AAAAAGGAGA | 0 | |
| GCTTTCAATG | AAGATGGCAA | 0 | |
| AGCCTTTATG | TATAGAGGAA | 0 | |
| TTTAGGTATA | GAATAGGGGA | 0 | |
| AATGTTTGCC | CATAA | TGGATACTGG | 5 |
| CGAAAGTCAT | TTCTA | TATAAATCGA | 5 |
| AAGACTACCC | GTTTG | AGCAGGAACG | 5 |
| GCTAAACCGC | CATTT | TTACGAACTG | 5 |
| GATAGCCATA | CTCTT | CTGATAACTT | 5 |
| CGTTTTTGCT | CTTAT | GGGAATCCCT | 5 |
| CTTTTTGTTT | CTTTT | ATGATTTCGT | 5 |
| ATCAAAGCCT | ATATG | GGCAATCGAG | 5 |
| GCAGGAATGT | AAAAA | GCAAATGCCG | 5 |
| CTTTTTGTTT | CTTTT | ATGATTTCGT | 5 |
| TTCGTGGGGC | AAGAT | ACGAATTTTT | 5 |
| GTTGATAACA | ATATG | TAGTTTGAAT | 5 |
| TCCAAGCATT | CTTCA | AAACGACTTC | 5 |
| GGAAATTTGC | CACAT | AGGAGTTATT | 5 |
| GTTAATTCAG | CTTTT | GGTCCATACT | 5 |
| TTCTCGGCGA | ACAAT | GGTATTTTCC | 5 |
| TTCTCGGCGA | ACAAT | GGTATTTTCC | 5 |
DNA sequence
GAGACCTTTGCACGGCGATTGGCGTATATTTTGTTTGTTAATTCATTGTATAATAGGAAGTTATTTCGTATATTTGAGTATTAAAAACAGCATAATATTC
CTCCCATGGCATACCAATCCAAGAATACCGATGAGCATGTAACATTTGCAGACGCACTCCTTTCAAAGCGTTATCGCAAAGCACAAAACGACTTCCTCAA
TCAGGTTGAGAGGCTTATCGATTGGCGTCCGATCAGGACGCTGATCAACAAGAAATACACGAAGCGACAAAATGCCATCGGCGCCCCGGCTTATGACGTG
ATTCTCTTATTCAAGATGTTGCTTTTGGAGACATGGTACAACCTCAGTGATTGTGCTTTGGAGGAGCGCATCAATGATTCAATCACCTTTTCCCGATTCT
TGGGACTGAAGATGGAAGAGGTATCTCCCGACCACAGCACCATCAGTCGATTTCGTTCGGCACTGACAGAGTTGGGGCTCATGGACAAACTATTGGCGCA
GTTTAACAAACAACTTTCCCGCCATCACATTTCGGTCAGGGAAGGGGTGCTTGTCGATGCAAGCCTTGTGGAGACGCCACATAAACCCAACGGAACCATT
ACGATTGAAGTCGCAGACGACAGAGAAGACAATCGGAGCGAGGCGGAAAAAGAGGCAGAGGAGGATTATCAAAAACAGGTTGTCCGCCGGCGTAAAGGGA
CGGATGAAGAAGCCCGTTGGGTGTACAAACAAAAGCGTTATCACTACGGATACAAAAAGCATTGTCTGACCAATGTTCAAGGCATTGTTCAAAAGGTGAT
AACGACTGCAGCGAACCGCAGTGACACGAAGGAGTTTATTCCGCTATTGCAGGGTGCAAACATACCTCAAGGCACAGCCGTCTTGGCGGACAAAGGATAT
GCTTGCGGGGAAAATCGTTCCTACCTGCAAACCCATCACCTTCAAGACGGCATCATGCACAAGGCACAACGCAACAGGGCATTGACCGAGGAAGAGAAGC
AAGGAAACAAAGCAATCAGTCCGATACGGAGCACCATCGAACGCACCTTTGGCAGTATTCGCCGGTGGTTTCATGGCGGACGATGTCGATACCGGGGACT
TGCCAAGACCCATACTCAAAACATTCTTGAAAGCATCGCCTTTAATTTATACAGAACCCCGGGGATAATTATGTCCTCATCTCTAGGATAAGGCATAACC
CCCCTTGAGGAGCTCGTGCAAGTAGCTCCGCAAGGGGGGATTTACAACTACTTTCACTCCTTACTGCCACCCTTTTCACTCGCTCCTTTTTTGCCAAGAA
CTCCTCTTCCCTCCATCTCCTTATTTTGCAAAGGTCTC
CTCCCATGGCATACCAATCCAAGAATACCGATGAGCATGTAACATTTGCAGACGCACTCCTTTCAAAGCGTTATCGCAAAGCACAAAACGACTTCCTCAA
TCAGGTTGAGAGGCTTATCGATTGGCGTCCGATCAGGACGCTGATCAACAAGAAATACACGAAGCGACAAAATGCCATCGGCGCCCCGGCTTATGACGTG
ATTCTCTTATTCAAGATGTTGCTTTTGGAGACATGGTACAACCTCAGTGATTGTGCTTTGGAGGAGCGCATCAATGATTCAATCACCTTTTCCCGATTCT
TGGGACTGAAGATGGAAGAGGTATCTCCCGACCACAGCACCATCAGTCGATTTCGTTCGGCACTGACAGAGTTGGGGCTCATGGACAAACTATTGGCGCA
GTTTAACAAACAACTTTCCCGCCATCACATTTCGGTCAGGGAAGGGGTGCTTGTCGATGCAAGCCTTGTGGAGACGCCACATAAACCCAACGGAACCATT
ACGATTGAAGTCGCAGACGACAGAGAAGACAATCGGAGCGAGGCGGAAAAAGAGGCAGAGGAGGATTATCAAAAACAGGTTGTCCGCCGGCGTAAAGGGA
CGGATGAAGAAGCCCGTTGGGTGTACAAACAAAAGCGTTATCACTACGGATACAAAAAGCATTGTCTGACCAATGTTCAAGGCATTGTTCAAAAGGTGAT
AACGACTGCAGCGAACCGCAGTGACACGAAGGAGTTTATTCCGCTATTGCAGGGTGCAAACATACCTCAAGGCACAGCCGTCTTGGCGGACAAAGGATAT
GCTTGCGGGGAAAATCGTTCCTACCTGCAAACCCATCACCTTCAAGACGGCATCATGCACAAGGCACAACGCAACAGGGCATTGACCGAGGAAGAGAAGC
AAGGAAACAAAGCAATCAGTCCGATACGGAGCACCATCGAACGCACCTTTGGCAGTATTCGCCGGTGGTTTCATGGCGGACGATGTCGATACCGGGGACT
TGCCAAGACCCATACTCAAAACATTCTTGAAAGCATCGCCTTTAATTTATACAGAACCCCGGGGATAATTATGTCCTCATCTCTAGGATAAGGCATAACC
CCCCTTGAGGAGCTCGTGCAAGTAGCTCCGCAAGGGGGGATTTACAACTACTTTCACTCCTTACTGCCACCCTTTTCACTCGCTCCTTTTTTGCCAAGAA
CTCCTCTTCCCTCCATCTCCTTATTTTGCAAAGGTCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1083 bp | 361 aa | 106 | 1188 | + | No |
Chemistry : DDE
ORF sequence :
MAYQSKNTDEHVTFADALLSKRYRKAQNDFLNQVERLIDWRPIRTLINKKYTKRQNAIGAPAYDVILLFKMLLLETWYNLSDCALEERINDSITFSRFLG
LKMEEVSPDHSTISRFRSALTELGLMDKLLAQFNKQLSRHHISVREGVLVDASLVETPHKPNGTITIEVADDREDNRSEAEKEAEEDYQKQVVRRRKGTD
EEARWVYKQKRYHYGYKKHCLTNVQGIVQKVITTAANRSDTKEFIPLLQGANIPQGTAVLADKGYACGENRSYLQTHHLQDGIMHKAQRNRALTEEEKQG
NKAISPIRSTIERTFGSIRRWFHGGRCRYRGLAKTHTQNILESIAFNLYRTPGIIMSSSLG
LKMEEVSPDHSTISRFRSALTELGLMDKLLAQFNKQLSRHHISVREGVLVDASLVETPHKPNGTITIEVADDREDNRSEAEKEAEEDYQKQVVRRRKGTD
EEARWVYKQKRYHYGYKKHCLTNVQGIVQKVITTAANRSDTKEFIPLLQGANIPQGTAVLADKGYACGENRSYLQTHHLQDGIMHKAQRNRALTEEEKQG
NKAISPIRSTIERTFGSIRRWFHGGRCRYRGLAKTHTQNILESIAFNLYRTPGIIMSSSLG
Blast result :
Comments
ISPg8 is 57% aa similar to ISNme1.
ISPg8 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 49 residues. The copy number in Porphyromonas gingivalis W83 is 22.
ISPg8 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 49 residues. The copy number in Porphyromonas gingivalis W83 is 22.
References
1] Nelson,K., Fleishmann,R., DeBoy,R., Paulsen,I., Fouts,D., Eisen,J.,
Daugherty,S., Dodson,R., Durkin,A., Gwinn,M., Haft,D., Kolonay,J., Nelson,W., White,O., Mason,T., Tallon,L., Gray,J., Granger,D., Tettelin,H., Dong,H., Galvin,J., Duncan,M., Dewhirst,F. and Fraser,C.(2003)J. Bacteriol. 185,5591-5601
Daugherty,S., Dodson,R., Durkin,A., Gwinn,M., Haft,D., Kolonay,J., Nelson,W., White,O., Mason,T., Tallon,L., Gray,J., Granger,D., Tettelin,H., Dong,H., Galvin,J., Duncan,M., Dewhirst,F. and Fraser,C.(2003)J. Bacteriol. 185,5591-5601