ISNsp1
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_003272 | ND | Nostoc sp. | Nostoc sp. PCC 7120 |
DNA section
IS Length : 1028 bp
Ends
IR Length : 16/17
IRL : ACGCGATGTGCGACTTATTGTTTCGTTACACAAGTTCGGTGTATGAACGA
IRR : ACGCCATGTGCGACTTAATATTCTGTAACAAGATCGTCGAACTGAAGAGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTATAACTTT | GATTTTTGGT | 0 | |
| CTCTTACTTT | AACACTAGTA | 0 | |
| TAAGGAGTTA | AAAATATATA | 0 | |
| TTGATTCATG | TATTTA | GTAATTTCCT | 6 |
| GATTTAGCAG | TAGATA | AATACTTTTC | 6 |
| ACCAATTACT | AAAGTA | GGCTTATTAA | 6 |
| TGATATTACC | TATGTT | TGCTTGGTGC | 6 |
DNA sequence
ACGCGATGTGCGACTTATTGTTTCGTTACACAAGTTCGGTGTATGAACGATTGAAAAGAAAATTCCCACAATCCGCACTGGAGCGAGAAAATCGTTACCA
GTGAATGGAATGCGGGAAAAATGTTTTCTATTGAAGAGTTTATCATTGCCGTATTTTGCTGTGTTGACGACGTGCTGATGGAAATCACCCAGATATACCC
AATCAAGAGACGAGGTTTTGCTCCGAGTTTAAGGGAGAGTGAAGTTTTAACAATGGAAGTAGTGGCAGAGTTTTTGGGAATAGATGCTGACAAAGACATT
TGGAAATACTTTCACCGTCACTGGTTAGGGCTATTTCCTCAGTTAAAGAGTCGCTATGCTTTTATTCGTCAAGCAGCTAATTGTTGGCAGTACAAGGAAC
TCATACAACAAAAATTAGCACAGTATTTAGGAGCATACTCTGATCAACTTCATTTGATTGATGGATTACCAATACCTTTGTGTGGCTTGAGTCGCGCTCC
CAACTGCCGAAGTTTTAAATCCCTTGCAGATTATGGTTTTTGTGCTGCTAAAAAACAGACGTACTATGGGTTTCATGGGCATTTAATCGTTAGTGGCACA
GGAGTTATTAGTGGATTTACCCTGACTCCGGCAAATGGCAGTGAACGGGACGCACTTTGGGATTTAATCACGCGAATTAAAGGTTTATTAATCGGAGATA
AGGGCTATTTAAGTCAGTTCTTGTCAGGAGAACTTAAAGAAGTGGGTATTACTTTACAAACCCCTTTGCGTTCTAATATGTCTGACTCTCGTAGCCGATC
TTCAGTGCGATTAATGCAACGCTTTCGTCGCCTAATTGAAACAGTAATTGGTCAATTAGTTGAGAGATTTCATATAGAGAAGATTCGAGCGAGAGATATG
TGGCATCTTACCAGTCGTCTCAATCGCAAGATTTTAGCTCATACCGTCTGTTTCTGGCTTAATCGCCACAATTGTGACCCTCTTCAGTTCGACGATCTTG
TTACAGAATATTAAGTCGCACATGGCGT
GTGAATGGAATGCGGGAAAAATGTTTTCTATTGAAGAGTTTATCATTGCCGTATTTTGCTGTGTTGACGACGTGCTGATGGAAATCACCCAGATATACCC
AATCAAGAGACGAGGTTTTGCTCCGAGTTTAAGGGAGAGTGAAGTTTTAACAATGGAAGTAGTGGCAGAGTTTTTGGGAATAGATGCTGACAAAGACATT
TGGAAATACTTTCACCGTCACTGGTTAGGGCTATTTCCTCAGTTAAAGAGTCGCTATGCTTTTATTCGTCAAGCAGCTAATTGTTGGCAGTACAAGGAAC
TCATACAACAAAAATTAGCACAGTATTTAGGAGCATACTCTGATCAACTTCATTTGATTGATGGATTACCAATACCTTTGTGTGGCTTGAGTCGCGCTCC
CAACTGCCGAAGTTTTAAATCCCTTGCAGATTATGGTTTTTGTGCTGCTAAAAAACAGACGTACTATGGGTTTCATGGGCATTTAATCGTTAGTGGCACA
GGAGTTATTAGTGGATTTACCCTGACTCCGGCAAATGGCAGTGAACGGGACGCACTTTGGGATTTAATCACGCGAATTAAAGGTTTATTAATCGGAGATA
AGGGCTATTTAAGTCAGTTCTTGTCAGGAGAACTTAAAGAAGTGGGTATTACTTTACAAACCCCTTTGCGTTCTAATATGTCTGACTCTCGTAGCCGATC
TTCAGTGCGATTAATGCAACGCTTTCGTCGCCTAATTGAAACAGTAATTGGTCAATTAGTTGAGAGATTTCATATAGAGAAGATTCGAGCGAGAGATATG
TGGCATCTTACCAGTCGTCTCAATCGCAAGATTTTAGCTCATACCGTCTGTTTCTGGCTTAATCGCCACAATTGTGACCCTCTTCAGTTCGACGATCTTG
TTACAGAATATTAAGTCGCACATGGCGT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 891 bp | 297 aa | 121 | 1011 | + | No |
Chemistry : DDE
ORF sequence :
MFSIEEFIIAVFCCVDDVLMEITQIYPIKRRGFAPSLRESEVLTMEVVAEFLGIDADKDIWKYFHRHWLGLFPQLKSRYAFIRQAANCWQYKELIQQKLA
QYLGAYSDQLHLIDGLPIPLCGLSRAPNCRSFKSLADYGFCAAKKQTYYGFHGHLIVSGTGVISGFTLTPANGSERDALWDLITRIKGLLIGDKGYLSQF
LSGELKEVGITLQTPLRSNMSDSRSRSSVRLMQRFRRLIETVIGQLVERFHIEKIRARDMWHLTSRLNRKILAHTVCFWLNRHNCDPLQFDDLVTEY
QYLGAYSDQLHLIDGLPIPLCGLSRAPNCRSFKSLADYGFCAAKKQTYYGFHGHLIVSGTGVISGFTLTPANGSERDALWDLITRIKGLLIGDKGYLSQF
LSGELKEVGITLQTPLRSNMSDSRSRSSVRLMQRFRRLIETVIGQLVERFHIEKIRARDMWHLTSRLNRKILAHTVCFWLNRHNCDPLQFDDLVTEY
Blast result :
Comments
ISNsp1 is 65% aa similar to ISAba825.
ISNsp1 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 46 residues. The copy number in Nostoc sp. PCC 7120 is 7.
ISNsp1 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 46 residues. The copy number in Nostoc sp. PCC 7120 is 7.
References
1] Kaneko,T., Nakamura,Y., Wolk,C.P., Kuritz,T., Sasamoto,S., Watanabe,A., Iriguchi,M., Ishikawa,A., Kawashima,K., Kimura,T., Kishida,Y., Kohara,M., Matsumoto,M., Matsuno,A., Muraki,A., Nakazaki,N., Shimpo,S., Sugimoto,M., Takazawa,M., Yamada,M., Yasuda,M. and Tabata,S.(2001)DNA Res. 8,205-213