ISNeu2
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004757 | ND | Nitrosomonas europaea | Nitrosomonas europaea ATCC 19718 |
DNA section
IS Length : 1000 bp
Ends
IR Length : 21/21
IRL : CTAACCTGAGTTCGGGATAAGAAAAGAGGAGTTGAGCGCCAGCCTTTTTG
IRR : CTAACCTGAGTTCGGGATAAGCCCTCAAGCTTTGACCAAGGTATTGACCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGAATAGGCT | TAC | ATGACAGTGC | 3 |
DNA sequence
CTAACCTGAGTTCGGGATAAGAAAAGAGGAGTTGAGCGCCAGCCTTTTTGCGTAAGATTATGTTTCCACAAACAAATCGATCGCATCCTGGAGACGCTCA
TGGACAGTTTAACCGAACTTTTTTGCCTCACAGATGATTTCTGCCACCAGTTCGAGCCTGCTTTAGAGCGCCATCTTCTGGAAGCAGGGGTAAAAAAGCG
CAAACGCCGCAGCGAGTTAGCTTATCGGAGTTAATGACCTTAACTGTTTTGTTCCATCAATTGCGCTTTCGCCAGTTCAAGAGTTTTTACTTGGTTTACG
TGTGTCGTCACTGGCAGGCTGAGTTTCCAAAGCTGCCAAGCTATCAACGATGTGTGGAACTCCTGCCCGCTGTGTAGCTCCTTTGGCAGCCTTATTCGAG
ATGCTCAAAGGCCAGTGTGACGGGATTTCCATCGCTGATGCTACTGCAATCGCCGTTTGCGATAACCGGCGCATCGCCCGCCATCGGGTCTCTGCTGACA
GCGCCAGACGCGGCAAGACTTCGATGGGCTGGTTCTATGGATTCAAACTCCACGCCATCATCAACTCCAGGGGTGAACTGATCCGTCTGCGGCTCACTGC
CGGTAATGTTGACGATCGTAAACCCATGCCCGACCTCTGTCAGGGACTGTTCGGCCAGTTGTTTGCCGATAAAGGCTACCTTGCCCAGTGGCTCACTGAA
ACACTGGACCGGCAAAACCTTCAACTCATTACTCCCTTCAAGAAAAACATGAAGCCTGCGCCTCGTACCGGGTTCGAGAAAGCCATTCTACGTCGCCGTT
CCCTGATTGAAACCGTCTTTGATGAATTGAAGAATCTTTGCCAGATCAAACACACCCGACATCGCTCTTTCTTCAATTTTGTCGTTAACCTAATGGCTGG
TATCGTCGCTTACTGTTTGTCCGATAACAAACCTACCTTGAATCTGACCAGGGTCAATACCTTGGTCAAAGCTTGAGGGCTTATCCCGAACTCAGGTTAG
TGGACAGTTTAACCGAACTTTTTTGCCTCACAGATGATTTCTGCCACCAGTTCGAGCCTGCTTTAGAGCGCCATCTTCTGGAAGCAGGGGTAAAAAAGCG
CAAACGCCGCAGCGAGTTAGCTTATCGGAGTTAATGACCTTAACTGTTTTGTTCCATCAATTGCGCTTTCGCCAGTTCAAGAGTTTTTACTTGGTTTACG
TGTGTCGTCACTGGCAGGCTGAGTTTCCAAAGCTGCCAAGCTATCAACGATGTGTGGAACTCCTGCCCGCTGTGTAGCTCCTTTGGCAGCCTTATTCGAG
ATGCTCAAAGGCCAGTGTGACGGGATTTCCATCGCTGATGCTACTGCAATCGCCGTTTGCGATAACCGGCGCATCGCCCGCCATCGGGTCTCTGCTGACA
GCGCCAGACGCGGCAAGACTTCGATGGGCTGGTTCTATGGATTCAAACTCCACGCCATCATCAACTCCAGGGGTGAACTGATCCGTCTGCGGCTCACTGC
CGGTAATGTTGACGATCGTAAACCCATGCCCGACCTCTGTCAGGGACTGTTCGGCCAGTTGTTTGCCGATAAAGGCTACCTTGCCCAGTGGCTCACTGAA
ACACTGGACCGGCAAAACCTTCAACTCATTACTCCCTTCAAGAAAAACATGAAGCCTGCGCCTCGTACCGGGTTCGAGAAAGCCATTCTACGTCGCCGTT
CCCTGATTGAAACCGTCTTTGATGAATTGAAGAATCTTTGCCAGATCAAACACACCCGACATCGCTCTTTCTTCAATTTTGTCGTTAACCTAATGGCTGG
TATCGTCGCTTACTGTTTGTCCGATAACAAACCTACCTTGAATCTGACCAGGGTCAATACCTTGGTCAAAGCTTGAGGGCTTATCCCGAACTCAGGTTAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 624 bp | 208 aa | 350 | 973 | + | No |
Chemistry : DDE
ORF sequence :
MCGTPARCVAPLAALFEMLKGQCDGISIADATAIAVCDNRRIARHRVSADSARRGKTSMGWFYGFKLHAIINSRGELIRLRLTAGNVDDRKPMPDLCQGL
FGQLFADKGYLAQWLTETLDRQNLQLITPFKKNMKPAPRTGFEKAILRRRSLIETVFDELKNLCQIKHTRHRSFFNFVVNLMAGIVAYCLSDNKPTLNLT
RVNTLVKA
FGQLFADKGYLAQWLTETLDRQNLQLITPFKKNMKPAPRTGFEKAILRRRSLIETVFDELKNLCQIKHTRHRSFFNFVVNLMAGIVAYCLSDNKPTLNLT
RVNTLVKA
Blast result :
Comments
ISNeu2 is 70% aa similar to IS1597.
ISNeu2 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed that the N-terminal part of the transposase is truncated, yet the DDE motif remained conserved: D(N2)-68-D(N3)-46-E(C1). The copy number in Nitrosomonas europaea ATCC 19718 is 1.
ISNeu2 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed that the N-terminal part of the transposase is truncated, yet the DDE motif remained conserved: D(N2)-68-D(N3)-46-E(C1). The copy number in Nitrosomonas europaea ATCC 19718 is 1.
References
[1]Chain,P., Lamerdin,J., Larimer,F., Regala,W., Lao,V., Land,M., Hauser,L., Hooper,A., Klotz,M., Norton,J., Sayavedra-Soto,L., Arciero,D., Hommes,N., Whittaker,M. and Arp,D.(2003)J. Bacteriol. 185,2759-2773