ISNeu1
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004757 | ND | Nitrosomonas europaea | Nitrosomonas europaea ATCC 19718 |
DNA section
IS Length : 996 bp
Ends
IR Length : 18/18
IRL : ACCTGAGTTCGGGATAAGAAAAGAGGAAGTGAGCGCCCGCCTTTTTGCGT
IRR : ACCTGAGTTCGGGATAAGCCCTTAAGCTTTAGCCAGGCTGTTGACCCTGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTGATCCG | TATCGGTA | GCAATCGC | 8 |
| ATGTCTGC | TATGGGTA | GGCATACT | 8 |
| GAAATCCT | TACTGTA | GCGCAAAT | 7 |
| CGATATGG | TAAATA | GAGAGCGC | 6 |
DNA sequence
ACCTGAGTTCGGGATAAGAAAAGAGGAAGTGAGCGCCCGCCTTTTTGCGTAAGATTATGTTTCCAAAAACAAATCAATCGCATTCAGGAGGCGCTCATGG
ACAGTTTAACTGAACTTTTTTGTCTCATAGATGATTTCTGCTGCCAATTCGAGCCCGCTTTAGAACGCCGCCTTCTGGAAACAGGGGTAAAGAAGCGCAA
GCGCTGCAGCGGGTTGAGTTTGTCGGAGTTGATGACCTTGACCGTTTTGTTTCATCAATTGCGCTTTCGCCAGTTCAAGAGTTTCTACCTGGTTTACGTA
TGCCGTCACTTGCAGGCTGAGTTTCCGAAGTTGCCGAGCTATCAACGATGTGTGGAACTCCTGCCCCGCTGTGTAGCTCCCCTGGCAGCCTTGTTCGAGA
TGCTCAAAGGCCAGTGTGACGGGATTTCCATTGCTGATGCTACCGCGATCGCCGTTTGCGATAACCGGCGTATCGCCCGCCATCGCGTCTTTGCCGACAG
CGCCAGACGCGGCAAGACTTCGATGGGCTGGTTCTATGGATTCAAACTCCATGCCATCATCAATTCCAGGGGTGAACTGATCCGTCTGCGGCTCACTGCC
GGTAATGTTGACGATCGTAAACCCATGCCCGATCTCTGTCAGGGACTGTTCGGCCAGTTGTTTGCCGACAAAGGCTACCTCGCCCAGTGGCTCACTGAAG
CACTGGACCAGCAAAACCTTCAACTCATTACTCCCCTCAGGAAAAACATGAGGCCTGTGCCTCGTACCAGGTTCGAGAAAGTCATTCTGCGTCGCCGTTC
CCTGATTGAAACCGTCTTTGATGAATTGAAGAATCTTTGCCAGATCGAGCATACCCGACACCGCTCTCTCTTCAATTTTATCGTTAACCTAATGGCTGGT
ATCGTCGCTTACTGTTTGTCCGATAACAAACCAACTTTGAATCTGACCAGGGTCAACAGCCTGGCTAAAGCTTAAGGGCTTATCCCGAACTCAGGT
ACAGTTTAACTGAACTTTTTTGTCTCATAGATGATTTCTGCTGCCAATTCGAGCCCGCTTTAGAACGCCGCCTTCTGGAAACAGGGGTAAAGAAGCGCAA
GCGCTGCAGCGGGTTGAGTTTGTCGGAGTTGATGACCTTGACCGTTTTGTTTCATCAATTGCGCTTTCGCCAGTTCAAGAGTTTCTACCTGGTTTACGTA
TGCCGTCACTTGCAGGCTGAGTTTCCGAAGTTGCCGAGCTATCAACGATGTGTGGAACTCCTGCCCCGCTGTGTAGCTCCCCTGGCAGCCTTGTTCGAGA
TGCTCAAAGGCCAGTGTGACGGGATTTCCATTGCTGATGCTACCGCGATCGCCGTTTGCGATAACCGGCGTATCGCCCGCCATCGCGTCTTTGCCGACAG
CGCCAGACGCGGCAAGACTTCGATGGGCTGGTTCTATGGATTCAAACTCCATGCCATCATCAATTCCAGGGGTGAACTGATCCGTCTGCGGCTCACTGCC
GGTAATGTTGACGATCGTAAACCCATGCCCGATCTCTGTCAGGGACTGTTCGGCCAGTTGTTTGCCGACAAAGGCTACCTCGCCCAGTGGCTCACTGAAG
CACTGGACCAGCAAAACCTTCAACTCATTACTCCCCTCAGGAAAAACATGAGGCCTGTGCCTCGTACCAGGTTCGAGAAAGTCATTCTGCGTCGCCGTTC
CCTGATTGAAACCGTCTTTGATGAATTGAAGAATCTTTGCCAGATCGAGCATACCCGACACCGCTCTCTCTTCAATTTTATCGTTAACCTAATGGCTGGT
ATCGTCGCTTACTGTTTGTCCGATAACAAACCAACTTTGAATCTGACCAGGGTCAACAGCCTGGCTAAAGCTTAAGGGCTTATCCCGAACTCAGGT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 876 bp | 292 aa | 97 | 972 | + | No |
Chemistry : DDE
ORF sequence :
MDSLTELFCLIDDFCCQFEPALERRLLETGVKKRKRCSGLSLSELMTLTVLFHQLRFRQFKSFYLVYVCRHLQAEFPKLPSYQRCVELLPRCVAPLAALF
EMLKGQCDGISIADATAIAVCDNRRIARHRVFADSARRGKTSMGWFYGFKLHAIINSRGELIRLRLTAGNVDDRKPMPDLCQGLFGQLFADKGYLAQWLT
EALDQQNLQLITPLRKNMRPVPRTRFEKVILRRRSLIETVFDELKNLCQIEHTRHRSLFNFIVNLMAGIVAYCLSDNKPTLNLTRVNSLAKA
EMLKGQCDGISIADATAIAVCDNRRIARHRVFADSARRGKTSMGWFYGFKLHAIINSRGELIRLRLTAGNVDDRKPMPDLCQGLFGQLFADKGYLAQWLT
EALDQQNLQLITPLRKNMRPVPRTRFEKVILRRRSLIETVFDELKNLCQIEHTRHRSLFNFIVNLMAGIVAYCLSDNKPTLNLTRVNSLAKA
Blast result :
Comments
ISNeu1 is 68% aa similar to ISPlu6.
ISNeu1 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed a DDE motif: D(N2)-68-D(N3)-46-E(C1). The copy number in Nitrosomonas europaea ATCC 19718 is 4.
ISNeu1 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed a DDE motif: D(N2)-68-D(N3)-46-E(C1). The copy number in Nitrosomonas europaea ATCC 19718 is 4.
References
[1]Chain,P., Lamerdin,J., Larimer,F., Regala,W., Lao,V., Land,M., Hauser,L., Hooper,A., Klotz,M., Norton,J., Sayavedra-Soto,L., Arciero,D., Hommes,N., Whittaker,M. and Arp,D.(2003)J. Bacteriol. 185,2759-2773