ISCef3
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004369 | ND | Corynebacterium efficiens | Corynebacterium efficiens YS-314 |
DNA section
IS Length : 989 bp
Ends
IR Length : 11/11
IRL : TAAGTCCTATTAGGACTCTCGCGCGCAGGTCTGTTCAACGAGGGCGGAGA
IRR : TAAGTCCTATTGATTTTTAGTGGTCGTAGGCGATCAGGGAGCGTTTGTTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTATATGAG | ATCATCTAGG | 0 |
DNA sequence
TAAGTCCTATTAGGACTCTCGCGCGCAGGTCTGTTCAACGAGGGCGGAGAGTGCTTGAGTTTTTGGTGTGAACAACAAACTAAACACTCTCCTGACCACA
CTCTACGTCACCCTGGTCGACCGTGTCCTGCCCGATCTGGACTACGACCGGACTCACCGCCCAGGGCACCGACCAGCCCTCGATGACGCCGAACTGCTGT
GCCTGGCCGTCGCCCAACACCTGCTGCACGGATCCTCCTCAGAATCCCGATGGGTGCGCTACGCCCGGGCACACCTGACCGGAATGTTCCCGGCGATGCC
CCAACAATCGGGCTACAACAAGCGCATCCGGGCCGCCGGCCCGTTGATCAGCGCGGTGATCACCGCCCTGGCCAAAGACACCGCCTCCTGGCACGAGATC
CTGCGACTACTCGACTCCACCCCGGTACCCTGCGGCACCTCAAGAAAGACAGTCAAACGCTCTGACCTGGTCGGGCATGCCGGCTACGGCTACTGCGCTT
CGCATTCGAGGTTCTTCTGGGGTTTTCGCCTGTATCTGGTCTGCACCCCGGACGGGATGCCGGTGATCTGGGGGCTGGCCAACCCGAAGATCGGCGAGCG
GGAAACCACCCAGGTCCTGCTTGATCATGACTGTCATCTGGTGCACGACGGGCAGGTCATCCTGGCGGACAAGGGCTTCGCCGGGAAAGAGTTCGAGGCG
TTTGTCACCGACGAGCTTGGCGCCCACCTCGTCCGTCCCGACCGCAAGGATGAAAAGCCCCGGTTCGGGGCCCTCGGTGGTATCCGCCAGTGGGTCGAGT
CCGTCTTCGACACGCTTAAAGGCCAACTCGGCCTCGAGCAGCACGGCGGGCGCACCGTCGAAGGCGTCTACGCCCGAGTCGCCGCGAAACTTTTGGCCCT
GGCCGCCGGGATCTGGCACAACTGGCTCATCGACGCCCCCAACAAACGCTCCCTGATCGCCTACGACCACTAAAAATCAATAGGACTTA
CTCTACGTCACCCTGGTCGACCGTGTCCTGCCCGATCTGGACTACGACCGGACTCACCGCCCAGGGCACCGACCAGCCCTCGATGACGCCGAACTGCTGT
GCCTGGCCGTCGCCCAACACCTGCTGCACGGATCCTCCTCAGAATCCCGATGGGTGCGCTACGCCCGGGCACACCTGACCGGAATGTTCCCGGCGATGCC
CCAACAATCGGGCTACAACAAGCGCATCCGGGCCGCCGGCCCGTTGATCAGCGCGGTGATCACCGCCCTGGCCAAAGACACCGCCTCCTGGCACGAGATC
CTGCGACTACTCGACTCCACCCCGGTACCCTGCGGCACCTCAAGAAAGACAGTCAAACGCTCTGACCTGGTCGGGCATGCCGGCTACGGCTACTGCGCTT
CGCATTCGAGGTTCTTCTGGGGTTTTCGCCTGTATCTGGTCTGCACCCCGGACGGGATGCCGGTGATCTGGGGGCTGGCCAACCCGAAGATCGGCGAGCG
GGAAACCACCCAGGTCCTGCTTGATCATGACTGTCATCTGGTGCACGACGGGCAGGTCATCCTGGCGGACAAGGGCTTCGCCGGGAAAGAGTTCGAGGCG
TTTGTCACCGACGAGCTTGGCGCCCACCTCGTCCGTCCCGACCGCAAGGATGAAAAGCCCCGGTTCGGGGCCCTCGGTGGTATCCGCCAGTGGGTCGAGT
CCGTCTTCGACACGCTTAAAGGCCAACTCGGCCTCGAGCAGCACGGCGGGCGCACCGTCGAAGGCGTCTACGCCCGAGTCGCCGCGAAACTTTTGGCCCT
GGCCGCCGGGATCTGGCACAACTGGCTCATCGACGCCCCCAACAAACGCTCCCTGATCGCCTACGACCACTAAAAATCAATAGGACTTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 906 bp | 301 aa | 68 | 973 | + | No |
Chemistry : DDE
ORF sequence :
MNNKLNTLLTTLYVTLVDRVLPDLDYDRTHRPGHRPALDDAELLCLAVAQHLLHGSSSESRWVRYARAHLTGMFPAMPQQSGYNKRIRAAGPLISAVITA
LAKDTASWHEILRLLDSTPVPCGTSRKTVKRSDLVGHAGYGYCASHSRFFWGFRLYLVCTPDGMPVIWGLANPKIGERETTQVLLDHDCHLVHDGQVILA
DKGFAGKEFEAFVTDELGAHLVRPDRKDEKPRFGALGGIRQWVESVFDTLKGQLGLEQHGGRTVEGVYARVAAKLLALAAGIWHNWLIDAPNKRSLIAYD
H
LAKDTASWHEILRLLDSTPVPCGTSRKTVKRSDLVGHAGYGYCASHSRFFWGFRLYLVCTPDGMPVIWGLANPKIGERETTQVLLDHDCHLVHDGQVILA
DKGFAGKEFEAFVTDELGAHLVRPDRKDEKPRFGALGGIRQWVESVFDTLKGQLGLEQHGGRTVEGVYARVAAKLLALAAGIWHNWLIDAPNKRSLIAYD
H
Blast result :
Comments
ISCef3 is 45% aa similar to ISLpl4.
ISCef3 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 42 residues. The copy number in Corynebacterium efficiens YS-314 is 5.
ISCef3 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 42 residues. The copy number in Corynebacterium efficiens YS-314 is 5.
References
[1]Nishio,Y., Nakamura,Y., Kawarabayasi,Y., Usuda,Y., Kimura,E., Sugimoto,S., Matsui,K., Yamagishi,A., Kikuchi,H., Ikeo,K. and Gojobori,T.(2003)Genome Res. 13,1572-1579