ISCef2
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_004369 | ND | Corynebacterium efficiens | Corynebacterium efficiens YS-314 |
DNA section
IS Length : 1017 bp
Ends
IR Length : 24/26
IRL : TCGCCTAGATGAGTAAGTCCTAATAGAGTTCCCCGACGAAAAGGTCTATA
IRR : TCACCTAGATGATTAAGTCCTAATAGGTTCAGTGATCATAAGCTGTCAGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAGAAATTA | CGGGATAAA | 0 |
DNA sequence
TCGCCTAGATGAGTAAGTCCTAATAGAGTTCCCCGACGAAAAGGTCTATACAAAAATACGGGGAGAGTGTTTGAGTTGAAGGTGTGAACAATAACCTAAA
CACTCTCCTGACCACACTCTACGTCACCTTAGTCGACGATGTCTTGCCTTCTTTAGGTATCAATCGCACCCACCGACCAGGTAGAAAACCAGCCCTGGAC
GACGCCGAGCTCCTCTGCCTGGTCATCGCCCAACACCTCCTGCACGGCACCTCATCGGAGACACGATGGGTGCGCTACGCCCACCAACACTTCAGTGAGA
TGTTTCCCGATATCCCGCAACAATCCGGGTATAACAAACGCGTCCGTGCCGCTAGTTCACTGATTTCGCTGGTGATCACTGCCCTGGCTCGAGATACCGC
CTCCTGGTACGACCTGTGCCGTTTGCTCGATTCCACGCCAATTCCGTGTGCGATGTCAAGAGAAACGGTGAAACGATCAGATCTGGCCGGGTATGCCAAC
TATGGGTATTGCGCGTCGCATTCGCGGTATTTCTGGGGGTTTCGTCTCTACCTGGTGTGTGCCCCGGATGGAATGCCGGTGATCTGGGGACTGGCTGATC
CAAAGATTGGGGAACGCGAGGCTGCCATGGTGTTATTAGCCCATGACCAGCATCTTCTTTCTACGGGCCAGGTGATTGTGGCGGATAAGGGGTTTGCCGG
TTGTGACTTTGAGGGGTTTATCTCCCACGAGCTGGGTGTGGAGTTGATTCGCCCGGATCGCAAGAATGAGGCTCACAGGTATGGGGATCTTGGCGGGATC
AGGCAGTGGGTGGAATCCGTGTTCGACACCCTCAAGGACCAGTTGGGATTGGAGCACCACCGGGCAAGAAGCCCTGGTGGTGTCTATGCCAAGGTGGCGG
GCAAGTTGTTGGCGTTAGCATCTGTGATCTGGCATAACTGGCGTATCGGTGCACCTGATAAGCGCTCACTGACAGCTTATGATCACTGAACCTATTAGGA
CTTAATCATCTAGGTGA
CACTCTCCTGACCACACTCTACGTCACCTTAGTCGACGATGTCTTGCCTTCTTTAGGTATCAATCGCACCCACCGACCAGGTAGAAAACCAGCCCTGGAC
GACGCCGAGCTCCTCTGCCTGGTCATCGCCCAACACCTCCTGCACGGCACCTCATCGGAGACACGATGGGTGCGCTACGCCCACCAACACTTCAGTGAGA
TGTTTCCCGATATCCCGCAACAATCCGGGTATAACAAACGCGTCCGTGCCGCTAGTTCACTGATTTCGCTGGTGATCACTGCCCTGGCTCGAGATACCGC
CTCCTGGTACGACCTGTGCCGTTTGCTCGATTCCACGCCAATTCCGTGTGCGATGTCAAGAGAAACGGTGAAACGATCAGATCTGGCCGGGTATGCCAAC
TATGGGTATTGCGCGTCGCATTCGCGGTATTTCTGGGGGTTTCGTCTCTACCTGGTGTGTGCCCCGGATGGAATGCCGGTGATCTGGGGACTGGCTGATC
CAAAGATTGGGGAACGCGAGGCTGCCATGGTGTTATTAGCCCATGACCAGCATCTTCTTTCTACGGGCCAGGTGATTGTGGCGGATAAGGGGTTTGCCGG
TTGTGACTTTGAGGGGTTTATCTCCCACGAGCTGGGTGTGGAGTTGATTCGCCCGGATCGCAAGAATGAGGCTCACAGGTATGGGGATCTTGGCGGGATC
AGGCAGTGGGTGGAATCCGTGTTCGACACCCTCAAGGACCAGTTGGGATTGGAGCACCACCGGGCAAGAAGCCCTGGTGGTGTCTATGCCAAGGTGGCGG
GCAAGTTGTTGGCGTTAGCATCTGTGATCTGGCATAACTGGCGTATCGGTGCACCTGATAAGCGCTCACTGACAGCTTATGATCACTGAACCTATTAGGA
CTTAATCATCTAGGTGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 906 bp | 301 aa | 84 | 989 | + | No |
Chemistry : DDE
ORF sequence :
MNNNLNTLLTTLYVTLVDDVLPSLGINRTHRPGRKPALDDAELLCLVIAQHLLHGTSSETRWVRYAHQHFSEMFPDIPQQSGYNKRVRAASSLISLVITA
LARDTASWYDLCRLLDSTPIPCAMSRETVKRSDLAGYANYGYCASHSRYFWGFRLYLVCAPDGMPVIWGLADPKIGEREAAMVLLAHDQHLLSTGQVIVA
DKGFAGCDFEGFISHELGVELIRPDRKNEAHRYGDLGGIRQWVESVFDTLKDQLGLEHHRARSPGGVYAKVAGKLLALASVIWHNWRIGAPDKRSLTAYD
H
LARDTASWYDLCRLLDSTPIPCAMSRETVKRSDLAGYANYGYCASHSRYFWGFRLYLVCAPDGMPVIWGLADPKIGEREAAMVLLAHDQHLLSTGQVIVA
DKGFAGCDFEGFISHELGVELIRPDRKNEAHRYGDLGGIRQWVESVFDTLKDQLGLEHHRARSPGGVYAKVAGKLLALASVIWHNWRIGAPDKRSLTAYD
H
Blast result :
Comments
ISCef2 is 46% aa similart to ISBs1.
ISCef2 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 42 residues. The copy number in Corynebacterium efficiens YS-314 is 2.
ISCef2 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 42 residues. The copy number in Corynebacterium efficiens YS-314 is 2.
References
[1]Nishio,Y., Nakamura,Y., Kawarabayasi,Y., Usuda,Y., Kimura,E., Sugimoto,S., Matsui,K., Yamagishi,A., Kikuchi,H., Ikeo,K. and Gojobori,T.(2003)Genome Res. 13,1572-1579