ISCth1
- Family IS982
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_009012 | ND | Clostridium thermocellum | Clostridium thermocellum ATCC 27405 |
DNA section
IS Length : 1151 bp
Ends
IR Length : 22/29
IRL : ACTTGTTGTGCTAGCTTTTTTTACAGGTAAAAATTGCGAATGAAAAACTC
IRR : ATCTGTTGTGCTACCTGATAGTTTACAGGCTCAAGGAATATTATGGCAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTTAATTTTATTA | TAATTTA | ATTAAATTACCAT | 7 |
| CCACAAAACAAGA | TAATTTA | ATTAAAATTATAG | 7 |
| AAGGAGTTATACT | TAAGTTA | GTTAATTAAGTGA | 7 |
| CAAGTTATATATG | TAAATTA | TATAAATTATAAG | 7 |
| AAGAAAGGAGAGC | TAACTTA | GAAAAGCTAAAAA | 7 |
| TTAAATTCTTATA | TAATTTA | AGAAACCGCCAAC | 7 |
| GCTGGTTGATAAT | AAAATTA | ATATTGTAATCCT | 7 |
DNA sequence
ACTTGTTGTGCTAGCTTTTTTTACAGGTAAAAATTGCGAATGAAAAACTCCAACATAATATTGTAACCTATCATTAAACACCAAACTAATGATAGGAGGT
CACAATATATGATGGAGCTTGATAAAAATTATTATATCAAAGAAATTGAAAACTTAAAAGACTTTGTAACTGTCGCCTACATCATAATTGACGACATTTA
CCAGAAGGTAACTCCACCACACATTGCAAATCGCTGTAACATCAATAATTCGGTTATGAGCGATAGTGAAATAATTACCATATGTATTGTTGGAGAGTTA
CTCACTATCGACTCTGAGAAAGCTTGGTTGGGTTTCTGTAAGAAGAATATGAGAGACCTGTTTCCCAAATTCTGTGACAGGACCAGGTTCAATAGAACCC
GCAGAAACCTGCACGCAGTAATTGAGGAAATCAGAAAAGAACTCTCAAACCTTACAGGGTATGCCCAGCAACCTTACCGGATAGTAGATAGTATTCCTAT
ACCGGTGTGCAAATTTGGAAGAGCCAAGTTCCATAAAACATTCCGTGGTTTCGGAGCAACTTATGGAAAATGTCCTTCCAAAAAAGAAATATATCTGGGC
TATAAGCTGCATATGCTTGCAACCCTGGATGGTTTTATAACTGATTTTGCAATTACACCTGCCAATGTTGATGATCGTGCTGGTGTATGGGACCTGATTG
ATTCTTACCGGCGGATAACCTTAATTGGAGATAAGGGATATATTGGAACAGAATTTGCTGTTGAACTAAAAGAGGAAAAGGAAATAGAGATCCTACCTGT
TAAAAGGAGCAACAGCAAGTTACAGTTTCCGAAAGCTATAAGGCAATTAATCTTCAAATTAAGGCGCCGGATAGAGACTTCGGCTTCTCAGCTTACCCAG
CAACTCAATATAGAGAAGGTGCTTGCAAAGTCGTACTGGGGATTTTTAGCCAGGGTAAAAACCAAGCTTTTAGCCTACAACTTATGTTATTACATCAATA
AGCTTATAGGCCGTGATATTAATTTTTCGAGGATCAAAGAGTTAGTATTTGGTTAAAAAATTTAATTCCTCAAACTCAGTAGGAATACTATAGGTGTTTG
AGTTGCCATAATATTCCTTGAGCCTGTAAACTATCAGGTAGCACAACAGAT
CACAATATATGATGGAGCTTGATAAAAATTATTATATCAAAGAAATTGAAAACTTAAAAGACTTTGTAACTGTCGCCTACATCATAATTGACGACATTTA
CCAGAAGGTAACTCCACCACACATTGCAAATCGCTGTAACATCAATAATTCGGTTATGAGCGATAGTGAAATAATTACCATATGTATTGTTGGAGAGTTA
CTCACTATCGACTCTGAGAAAGCTTGGTTGGGTTTCTGTAAGAAGAATATGAGAGACCTGTTTCCCAAATTCTGTGACAGGACCAGGTTCAATAGAACCC
GCAGAAACCTGCACGCAGTAATTGAGGAAATCAGAAAAGAACTCTCAAACCTTACAGGGTATGCCCAGCAACCTTACCGGATAGTAGATAGTATTCCTAT
ACCGGTGTGCAAATTTGGAAGAGCCAAGTTCCATAAAACATTCCGTGGTTTCGGAGCAACTTATGGAAAATGTCCTTCCAAAAAAGAAATATATCTGGGC
TATAAGCTGCATATGCTTGCAACCCTGGATGGTTTTATAACTGATTTTGCAATTACACCTGCCAATGTTGATGATCGTGCTGGTGTATGGGACCTGATTG
ATTCTTACCGGCGGATAACCTTAATTGGAGATAAGGGATATATTGGAACAGAATTTGCTGTTGAACTAAAAGAGGAAAAGGAAATAGAGATCCTACCTGT
TAAAAGGAGCAACAGCAAGTTACAGTTTCCGAAAGCTATAAGGCAATTAATCTTCAAATTAAGGCGCCGGATAGAGACTTCGGCTTCTCAGCTTACCCAG
CAACTCAATATAGAGAAGGTGCTTGCAAAGTCGTACTGGGGATTTTTAGCCAGGGTAAAAACCAAGCTTTTAGCCTACAACTTATGTTATTACATCAATA
AGCTTATAGGCCGTGATATTAATTTTTCGAGGATCAAAGAGTTAGTATTTGGTTAAAAAATTTAATTCCTCAAACTCAGTAGGAATACTATAGGTGTTTG
AGTTGCCATAATATTCCTTGAGCCTGTAAACTATCAGGTAGCACAACAGAT
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 948 bp | 315 aa | 109 | 1056 | + | No |
Chemistry : DDE
ORF sequence :
MMELDKNYYIKEIENLKDFVTVAYIIIDDIYQKVTPPHIANRCNINNSVMSDSEIITICIVGELLTIDSEKAWLGFCKKNMRDLFPKFCDRTRFNRTRRN
LHAVIEEIRKELSNLTGYAQQPYRIVDSIPIPVCKFGRAKFHKTFRGFGATYGKCPSKKEIYLGYKLHMLATLDGFITDFAITPANVDDRAGVWDLIDSY
RRITLIGDKGYIGTEFAVELKEEKEIEILPVKRSNSKLQFPKAIRQLIFKLRRRIETSASQLTQQLNIEKVLAKSYWGFLARVKTKLLAYNLCYYINKLI
GRDINFSRIKELVFG
LHAVIEEIRKELSNLTGYAQQPYRIVDSIPIPVCKFGRAKFHKTFRGFGATYGKCPSKKEIYLGYKLHMLATLDGFITDFAITPANVDDRAGVWDLIDSY
RRITLIGDKGYIGTEFAVELKEEKEIEILPVKRSNSKLQFPKAIRQLIFKLRRRIETSASQLTQQLNIEKVLAKSYWGFLARVKTKLLAYNLCYYINKLI
GRDINFSRIKELVFG
Blast result :
Comments
ISCth1 is 53% aa similar to ISAba825.
ISCth1 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 42 residues.
ISCth1 was found by screening completely sequenced genomes for seqences homologous to the ISScr1 transposase using BLASTP. Multiple alignments revealed conserved D(N3) and E(C1) amino acids separated by 42 residues.
References
1] Daniel de Palmenaer (2005) Direct submission.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Wu,J.H.D., Newcomb,M. and Richardson,P. (2007) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Wu,J.H.D., Newcomb,M. and Richardson,P. (2007) Direct submission GenBank.