ISMysp1
- Family IS110
- Group
Isoform IS1110Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| DQ983918 | ND | Mycobacterium sp. | Mycobacterium sp. SNP11 |
DNA section
IS Length : 1461 bp
Ends
Left end : TCCTTAAGTTCGTAGTTGCAGTTACTTCGTGTTGTTACGTTGGGCTGTGACCCGGGTGGGTCGGGTGAGGCTGCATGGTCGTAGCCGGCCCTGGTGGTGC
Right end : ACGGCGCACCAACGTTCTCTACGCCCTCATCCGTGACAACCGGACCTGGCAACCTGATTCACCCCCGGTCACCGCCGCGGCTTGACTTCTTCATTGAGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ACGCAAGTCG | TCCTTTCGACGAT | 0 | |
| ATCACAGACG | TCCTTTCACGATT | 0 | |
| TCATGGACAC | TCCTTTCTGCGAG | 0 |
DNA sequence
TCCTTAAGTTCGTAGTTGCAGTTACTTCGTGTTGTTACGTTGGGCTGTGACCCGGGTGGGTCGGGTGAGGCTGCATGGTCGTAGCCGGCCCTGGTGGTGC
GGCTTGAAAGGACTGGCCGCCCGGCCTGCCTGGACTCCTCTGGCCGCTGATTGAGATCTGCGATGTGATCGCTTGTTTAAGGAAATGCTGGCGGGGTTGT
CCATGGGGATCATGTTGGTAGACAGGCGAAAAGAGGGGTGATGACGACGGGCCAGGTGTGGGCTGGAGTGGATGTTGGTAAAGAGCATCACTGGGTTTGT
GCGGTCGACGACAGCGGGAAGGTGGTGTTGTCGCGCCGGCTGGTCAACGACGAGCAACCGATCCGGGAGCTCGTCGCTGAAATCGACGAGCTGGCCGAGC
GAGTGTCGTGGACGGTGGACCTGACCACGGTGTATGCCGCACTGGTGTTGACGGTGCTGGCCGACGCCGGCAAAACGGTCCGGTATCTGGCCGGCCGGGC
GGTGTGGCAGGCCTCGGCGACCTACCGCGGTGGGGAGGCCAAAACCGATGCCAAAGACGCTCGGGTGATCGCCGACCAGGCGCGCATGCGCGGCCAGGAC
TTGCCCGTTCTGCATCCCGATGATGACTTGATCAGCGAGTTGCGGATGCTCACCGGCCATCGCGCCGACCTGGTGGCCGACCGCACCCGCACGATCAACC
GGCTGCGCCAGCAACTCGTCGCGGTCTGCCCGGCCCTGGAACGAGCCGCCCAACCGAGCCAAGACCGTGGCTGGGTGATACTGCTGGCGCGCTACCAGCG
TCCCAAAGCGATTCGACAAAGCGGTGTTTCGCGGCTGACCAAAGTCTTGACCGATGCCGGTGTGCGCAACGCCGCCTCGATCGCGGCGGCTGCGGTGGCT
GCAGTGAAAACCCAGACGATGCGCCTGCCCGGCGAAGAGGTGGCCGCCGGGTTGATCGCTGATCTGGCGCGGGAGGTGATCGCCCTCGATGACCGCATCA
AGACCACCGACGCCGACATCGAGGGCCGATTTCGCCGCCATCCACTCGCCGAGGTGATCACCAGCATGCCCGGCATAGGATTTCGGCTGGGCGCCGAATT
CCTCGCCGCCGTTGGTGATCCCGCGCTGATCGGATCGGCTGACCAACTCGCGGCCTGGGCCGGCCTGGCGCCAGTGTCTCGAGACTCCGGAAAGCGCACC
GGACGACTGCCACACCCCCAAGCGTTACAGTCGCCGACTGCGCCGAGTCATGTACATGTCGGCGTTGACCGCGATCCGCTGCGACCCAGATTCCAAGGCC
TATTACCAGCGCAAACGAGACGAAGGAAGCGGCCGATCCCCGCCACCATCTGCCTGGCGCGACGGCGCACCAACGTTCTCTACGCCCTCATCCGTGACAA
CCGGACCTGGCAACCTGATTCACCCCCGGTCACCGCCGCGGCTTGACTTCTTCATTGAGAG
GGCTTGAAAGGACTGGCCGCCCGGCCTGCCTGGACTCCTCTGGCCGCTGATTGAGATCTGCGATGTGATCGCTTGTTTAAGGAAATGCTGGCGGGGTTGT
CCATGGGGATCATGTTGGTAGACAGGCGAAAAGAGGGGTGATGACGACGGGCCAGGTGTGGGCTGGAGTGGATGTTGGTAAAGAGCATCACTGGGTTTGT
GCGGTCGACGACAGCGGGAAGGTGGTGTTGTCGCGCCGGCTGGTCAACGACGAGCAACCGATCCGGGAGCTCGTCGCTGAAATCGACGAGCTGGCCGAGC
GAGTGTCGTGGACGGTGGACCTGACCACGGTGTATGCCGCACTGGTGTTGACGGTGCTGGCCGACGCCGGCAAAACGGTCCGGTATCTGGCCGGCCGGGC
GGTGTGGCAGGCCTCGGCGACCTACCGCGGTGGGGAGGCCAAAACCGATGCCAAAGACGCTCGGGTGATCGCCGACCAGGCGCGCATGCGCGGCCAGGAC
TTGCCCGTTCTGCATCCCGATGATGACTTGATCAGCGAGTTGCGGATGCTCACCGGCCATCGCGCCGACCTGGTGGCCGACCGCACCCGCACGATCAACC
GGCTGCGCCAGCAACTCGTCGCGGTCTGCCCGGCCCTGGAACGAGCCGCCCAACCGAGCCAAGACCGTGGCTGGGTGATACTGCTGGCGCGCTACCAGCG
TCCCAAAGCGATTCGACAAAGCGGTGTTTCGCGGCTGACCAAAGTCTTGACCGATGCCGGTGTGCGCAACGCCGCCTCGATCGCGGCGGCTGCGGTGGCT
GCAGTGAAAACCCAGACGATGCGCCTGCCCGGCGAAGAGGTGGCCGCCGGGTTGATCGCTGATCTGGCGCGGGAGGTGATCGCCCTCGATGACCGCATCA
AGACCACCGACGCCGACATCGAGGGCCGATTTCGCCGCCATCCACTCGCCGAGGTGATCACCAGCATGCCCGGCATAGGATTTCGGCTGGGCGCCGAATT
CCTCGCCGCCGTTGGTGATCCCGCGCTGATCGGATCGGCTGACCAACTCGCGGCCTGGGCCGGCCTGGCGCCAGTGTCTCGAGACTCCGGAAAGCGCACC
GGACGACTGCCACACCCCCAAGCGTTACAGTCGCCGACTGCGCCGAGTCATGTACATGTCGGCGTTGACCGCGATCCGCTGCGACCCAGATTCCAAGGCC
TATTACCAGCGCAAACGAGACGAAGGAAGCGGCCGATCCCCGCCACCATCTGCCTGGCGCGACGGCGCACCAACGTTCTCTACGCCCTCATCCGTGACAA
CCGGACCTGGCAACCTGATTCACCCCCGGTCACCGCCGCGGCTTGACTTCTTCATTGAGAG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1203 bp | 401 aa | 241 | 1443 | + | No |
Chemistry : DEDD
ORF sequence :
MTTGQVWAGVDVGKEHHWVCAVDDSGKVVLSRRLVNDEQPIRELVAEIDELAERVSWTVDLTTVYAALVLTVLADAGKTVRYLAGRAVWQASATYRGGEA
KTDAKDARVIADQARMRGQDLPVLHPDDDLISELRMLTGHRADLVADRTRTINRLRQQLVAVCPALERAAQPSQDRGWVILLARYQRPKAIRQSGVSRLT
KVLTDAGVRNAASIAAAAVAAVKTQTMRLPGEEVAAGLIADLAREVIALDDRIKTTDADIEGRFRRHPLAEVITSMPGIGFRLGAEFLAAVGDPALIGSA
DQLAAWAGLAPVSRDSGKRTGRLPHPQALQSPTAPSHVHVGVDRDPLRPRFQGLLPAQTRRRKRPIPATICLARRRTNVLYALIRDNRTWQPDSPPVTAA
A
KTDAKDARVIADQARMRGQDLPVLHPDDDLISELRMLTGHRADLVADRTRTINRLRQQLVAVCPALERAAQPSQDRGWVILLARYQRPKAIRQSGVSRLT
KVLTDAGVRNAASIAAAAVAAVKTQTMRLPGEEVAAGLIADLAREVIALDDRIKTTDADIEGRFRRHPLAEVITSMPGIGFRLGAEFLAAVGDPALIGSA
DQLAAWAGLAPVSRDSGKRTGRLPHPQALQSPTAPSHVHVGVDRDPLRPRFQGLLPAQTRRRKRPIPATICLARRRTNVLYALIRDNRTWQPDSPPVTAA
A
Blast result :
Comments
ISMysp1 is 83% aa similar to IS1110.
Insertion sequence IS1110 was discovered for the first time in 1994 during a study of plasmid incidence in AIDS-derived Mycobacterium avium strains [1]. This element had thus far been detected in most human, veterinary, and environmental Mycobacterium avium isolates, but not in other Mycobacterium species such as M. bovis, M. tuberculosis, M. xenopi, M. kansasii and M. gordonae [1, 2, 3].During study of a polycyclic aromatic hydrocarbon-degrading gene cluster in Mycobacterium sp. SNP11, which is a fast-growing strain related to Mycobacterium gilvum, a DNA segment with very high similarities to the IS1110 element of Mycobacterium avium was identified [4]. This element, named ISMysp1 by the IS database curators, was found in three copies in the genome of Mycobacterium sp. SNP11. PCR and hybridization experiments revealed that this element is commonly found in fast-growing Mycobacterium strains [4].
Insertion sequence IS1110 was discovered for the first time in 1994 during a study of plasmid incidence in AIDS-derived Mycobacterium avium strains [1]. This element had thus far been detected in most human, veterinary, and environmental Mycobacterium avium isolates, but not in other Mycobacterium species such as M. bovis, M. tuberculosis, M. xenopi, M. kansasii and M. gordonae [1, 2, 3].During study of a polycyclic aromatic hydrocarbon-degrading gene cluster in Mycobacterium sp. SNP11, which is a fast-growing strain related to Mycobacterium gilvum, a DNA segment with very high similarities to the IS1110 element of Mycobacterium avium was identified [4]. This element, named ISMysp1 by the IS database curators, was found in three copies in the genome of Mycobacterium sp. SNP11. PCR and hybridization experiments revealed that this element is commonly found in fast-growing Mycobacterium strains [4].
References