ISSag10

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s) MtnLNU
Accession numberTranspositionOriginHost
AY928180 ND Streptococcus agalactiae
Streptococcus agalactiae UCN36
DNA section
IS Length : 1724 bp

Ends


IR Length : 22/25

IRL : GGCTCTGTCACAACAAATGGTTGATTTTTGACGAGGAATAGCGTATAATA
IRR : GGCTATGTCACAACAAACAGTTGATAAATAGCCTTTTTATAGGGGAGTAT

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNNNTTATTTTTNNNNNNNNNN8

DNA sequence

GGCTCTGTCACAACAAATGGTTGATTTTTGACGAGGAATAGCGTATAATAATAGTAAGAAACAGTACCACCGAAGGAGGTAATTGGATATGCCTACTATC
AAAGACGCATTAGATATTATCGGTAAGTTGACTGTCGCAGAGCAGGAAAGCCTTAAAACAATGCTTTTAAGTCCTGCCTTTGTAAAGTCTTTGAATATTG
AAGATTTCGCAGCAAAGGAACGCTTTGCAAATGGTCGTGTATGCCCTCTTTGTGGCTGTATCCATGTGGTTCGCAATGGTCATCGTAAAGATGGCACACA
GCGATATGTATGTAAGGATTGTGGCAAGTCCTTCGTGATTGCTACGAACTCCATTGTGTCTGGTACAAGAAAAGACTTGTCCGTGTGGGAGCAGTACATT
GATTGTATGATGAATGGCTTATCCATTCGTAAGACTGCTGTTGCTTGTGGGATTCACAGAAACACCGCATTCCTTTGGAGACACAAGATTTTGGATGCAC
TTCAGAATATGGCAGACGATGTTACCCTTGACGGCATTATTGAGGCTGACGAAACTTTTTTCGCCATCTCGTACAAGGGCAATCATAGCAAGAGTAAGAC
ATTTGCTATGCCACGCAAGGCTCATAAGCGTGGTCATTCTACACATATCAGAGGCTTGTCCCAAGAAAAGGTATGTGTTCCTTGTGCGGTTAATAGGAAT
GGCTTGTCTATCTCCAAGATTACGAATACTGGTAGAGTTTCTACAAGAGATTTACATCATATTTATGATGGTAGGATTAAGACCAATTCCACTCTTGTTA
CGGACAAGATGAACTCCTATGTGAGATTTACAAATGCCAATGGCATTGACCTTGTGCAGTTAAAGACTGGCAAAGCCAAGAAAGGCATTTATAATATCCA
ACATATCAATAGCTACCATAGCCAGCTAAAGAGGTTTATGCGTGGCTTTAACGGTGTTTCTACCAAGTATCTGAACAACTATCTTGTGTGGAATAACCTT
GTAAATTACGCCAAAGAAAGCGACATGGAGAAAAGGAACATCTTCTTAACTTTCGTTTTGGCAACATTGAAAACTGCTAAATGCAGAGATTTATCAAACA
GACCAGCAGTTCCTCTGGTCGCCTAATTAGAATTTGTGGAGATGATAAGATGGTCAATATAACAGATGTAAACCAGATTTTCCAATTTGCAATAGATGCG
GAGATTAAAGTCTTTCTTGATGGTGGCTGGGGTGTAGATGCTCTTCTTGGATATCAGTCAAGAGCCCATAATGATATTGACATTTTTGTAGAAAAGAACG
ATTATCAGAACTTTATAGAAATAATGAAAGCTAATGGCTTTTATGAGATTAAGATGGAATATACAACATTGAACCATACTGTATGGGAAGATTTGAAAAA
CAGAATTATTGATTTGCATTGTTTTGAATATACGGACGAAGGTGAAATTCTTTATGATGGGGATTGTTTTCCGGTAGAAACTCTTTCGGGTAAAGGAAGA
ATTGAGGAAATAGAGGTTTCCTGTATTGAACCATATAGTCAAGTAATGTTCCATCTGGGATACGAGTTTGATGAAAATGATGCACATGATGTGAAGTTAT
TGTGTGAGACACTTCATATCGAAATTCCAAATGAGTATAGATAACTGCAAATAACAGTTTGTAGGGGAGTTCTGATACTCCCCTATAAAAAGGCTATTTA
TCAACTGTTTGTTGTGACATAGCC
Protein section
ORF number : 2

 

ORF 2
LengthBeginEndStrandFusion ORF
495 bp164 aa11501644+No
ORF function : Passenger Gene
Annotation : O-lincosamide nucleotidyltransferaseDescription : Antibiotic resistance

ORF sequence :

MVNITDVNQIFQFAIDAEIKVFLDGGWGVDALLGYQSRAHNDIDIFVEKNDYQNFIEIMKANGFYEIKMEYTTLNHTVWEDLKNRIIDLHCFEYTDEGEI
LYDGDCFPVETLSGKGRIEEIEVSCIEPYSQVMFHLGYEFDENDAHDVKLLCETLHIEIPNEYR

 

Blast result :
ORF 1
LengthBeginEndStrandFusion ORF
1038 bp345 aa891126+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPTIKDALDIIGKLTVAEQESLKTMLLSPAFVKSLNIEDFAAKERFANGRVCPLCGCIHVVRNGHRKDGTQRYVCKDCGKSFVIATNSIVSGTRKDLSVW
EQYIDCMMNGLSIRKTAVACGIHRNTAFLWRHKILDALQNMADDVTLDGIIEADETFFAISYKGNHSKSKTFAMPRKAHKRGHSTHIRGLSQEKVCVPCA
VNRNGLSISKITNTGRVSTRDLHHIYDGRIKTNSTLVTDKMNSYVRFTNANGIDLVQLKTGKAKKGIYNIQHINSYHSQLKRFMRGFNGVSTKYLNNYLV
WNNLVNYAKESDMEKRNIFLTFVLATLKTAKCRDLSNRPAVPLVA

 

Blast result :
Comments
ISSag10 is 58% aa similar to ISBwe1
The second ORF is the gene of O-lincosamide nucleotidyltransferase.
References
1] Achard,A., Villers,C., Pichereau,V. and Leclercq,R. (2005) Antimicrob. Agents Chemother. 49 (7), 2716-2719