ISPto1

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AAPR01000036 ND Psychroflexus torquis
Psychroflexus torquis ATCC 700755
DNA section
IS Length : 1081 bp

Ends


IR Length : 20/27

IRL : GGCTCTAGTTTAATACAGCTATTATATGTATATTTAGATTATGAACATAG
IRR : GGCTCTAGTTTAGGTCTGCGTTGATATTAAGTTTTCATATCTAGATTCGA

Insertion site


Left flankDirect repeatRight flankDR Length
TTTATTTCACTTAATTGTTTTCTTAAATCCGAATTT8

DNA sequence

GGCTCTAGTTTAATACAGCTATTATATGTATATTTAGATTATGAACATAGATAATCTAAAAGAGGAAATACTATCACTATCCACTCTAGATCGTGATAAT
CTGTTAAAGGATATTACAGATTCACTTGAGCATAATCAATTGGTTGAGCGGGCTTCCCGTCGCCATATTTTAGATAATAAAATGGGCGGATGCCCACATT
GTTTACATGAAAAATATGTTCGTTTTGGAGTTGACAAAGGCTCGCAGCGCTATAAGTGCAAGTCTTGCAATAGAAGCTTTACTGAATATACTGGCACTTG
GATGGCGGGACTTCAAAGAAAGGATATGATTTCATCTTATTTAAGTCTTATGGTTCAAGAAAAAAGTTTAGATAAAATAAGTTCAGAATTAGGCATCAAT
AAAAAGACAGCCTTTGATTGGCGTCATAAGATATTAGCTTCATTCGATACTAAAAATGACGATGACCAAGACAACTTTACAGGTATTACAGAGAGTGACG
AAACCTTTTTCCTAAGATCAGAAAAAGGTATGGAGGTAAAAGATAGGGAATCAAGAAAAAGAGGCGGTAAGTCTAAAAAGAGAGGTATAAGTAAAGATCA
AGTTGCGGTAATTGTAACACAGGATAGAAAATCAACATTAGACCTTAGCGTGGCTAAACTCGGTCGAATAGGAAAAGTAGATATAGAAAATGCTATCGGT
AAACGTGTTATAAAGGATATAACCATATTGTGTAGCGATGCCCATCACAGTTATAAAGGGTTTGCCAAAGATAGCGAAACAGAGTTCCACATCGTAAATG
CATCAAAAGGAGAAAGAGTAAAAGGAAAGTATCACATACAACACGTTAATTCTACTCACAATAGAGTTAAAAAATGGATTGAAAATACCTTTTGGGGAGT
ATCAACAAAATATCTACAACAATATATGAATTGGTATCGAATAAAGGAAAATATAAAGTCTAGAAGCGATAGAGCCAACGCCTTTGTGGAAGAAACAATT
GCTCTAGGTACATTGAAACGGTATAATCAAATCGAATCTAGATATGAAAACTTAATATCAACGCAGACCTAAACTAGAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1032 bp343 aa411072+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNIDNLKEEILSLSTLDRDNLLKDITDSLEHNQLVERASRRHILDNKMGGCPHCLHEKYVRFGVDKGSQRYKCKSCNRSFTEYTGTWMAGLQRKDMISSY
LSLMVQEKSLDKISSELGINKKTAFDWRHKILASFDTKNDDDQDNFTGITESDETFFLRSEKGMEVKDRESRKRGGKSKKRGISKDQVAVIVTQDRKSTL
DLSVAKLGRIGKVDIENAIGKRVIKDITILCSDAHHSYKGFAKDSETEFHIVNASKGERVKGKYHIQHVNSTHNRVKKWIENTFWGVSTKYLQQYMNWYR
IKENIKSRSDRANAFVEETIALGTLKRYNQIESRYENLISTQT

 

Blast result :
Comments
ISPto1 is 60% aa similar to ISBwe1.
References
1] Bowman,J., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.