ISfinder Mirror Site

ISCku1

Family IS1595
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CT573071	ND	Candidatus Kuenenia	Candidatus Kuenenia stuttgartiensis

DNA section

IS Length : 1008 bp

Ends

IR Length : 27/29

IRL : AATGGTTATTGAGTTAACACAGCAAAACTCTTAAACTTTATATAGAAACA
IRR : AATGGTTATTGAGTTAACTCAGCAAAGCAAATGGCCCTGGAAATTGTACA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TGACATCATA	TTAAATTT	TAATTAAATGAATTAA	8

DNA sequence

AATGGTTATTGAGTTAACACAGCAAAACTCTTAAACTTTATATAGAAACAATTTACACATAAAGGATAGGAGGAAATTTACCATGGAAAAGCGATATAAA
GAAATAAGTTTTTTTGATTTTCAGAAACAATATAAAACACAAGAGGATTGCGAAAAGAAGCTTTTTGAAATTCGTTGGCCCCAGGGATTTGTTTGTCCAG
TATGTGGACATCAGAAGTATTATTTGATCTCTGGGAGAAAATTGTATCAATGTGTGAGTTGTAAACATCAAACATCCCTGACAGCAGGTACTGTAATGCA
TAGGAGCAGAACGCCACTGTTGTTCTGGTTCTGGACGATCTACCTGGTAAGTACTGATAAACGAGGGATTTCTGCCCTTGGACTTTCCAAAAAACTCGGT
TTGAGCTACTGGAAGGCGTGGACGATGCTGCATAAGATACGTAGGGCAATGGGAGACAGGGATTCTACGTATCAACTGGCAGGAATTGTGGAAATTGATG
ATGCATTTTTTGGCACTTCTCAGAAAGCAAAGAGTAAGCGTAGCAAACGAGGCAGGGGCACTTCCAAAACGGTAGTACTCGTTGAGGTGTCTCTTTACGA
TAATGCCGTTGGTTTTGCCAAGATGACTGTCGTAGGCAGCAAGGTAGATAGTGAAACGATTGATCAGGTAATAAAGGCAGATATCAAAGGAGTCCAGACG
GTGAAAACCGATGGACTGCCTGCTTACGCGGTTGTTAGTAAAAGTGGGCATACTCACCAACAAGAAATTGTTAAAGGGAAAAATGCCCATGAAGTACTGA
AATGGACGCATATGTTAATTTCTAATGCAAAAAGCTTTTTATTAGGAACTTTCCATAGAATGGGAAAGAAACATTTACAATCCTATCTTGATGAATTCTG
TTATCGTTTTAACCGAAGAAAATGGGAATCTCAGCTTTTTGACAGATTAATTACTGCTTGTACAATTTCCAGGGCCATTTGCTTTGCTGAGTTAACTCAA
TAACCATT

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
921 bp	306 aa	83	1003	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MEKRYKEISFFDFQKQYKTQEDCEKKLFEIRWPQGFVCPVCGHQKYYLISGRKLYQCVSCKHQTSLTAGTVMHRSRTPLLFWFWTIYLVSTDKRGISALG
LSKKLGLSYWKAWTMLHKIRRAMGDRDSTYQLAGIVEIDDAFFGTSQKAKSKRSKRGRGTSKTVVLVEVSLYDNAVGFAKMTVVGSKVDSETIDQVIKAD
IKGVQTVKTDGLPAYAVVSKSGHTHQQEIVKGKNAHEVLKWTHMLISNAKSFLLGTFHRMGKKHLQSYLDEFCYRFNRRKWESQLFDRLITACTISRAIC
FAELTQ

Blast result :

Comments

ISCku1 is 59% aa similar to ISPpr5.

References

1] Strous,M., Pelletier,E., Mangenot,S., Rattei,T., Lehner,A.,
Taylor,M.W., Horn,M., Daims,H., Bartol-Mavel,D., Wincker,P.,
Barbe,V., Fonknechten,N., Vallenet,D., Segurens,B., Schenowitz-Truong,C., Medigue,C., Collingro,A., Snel,B., Dutilh,B.E., Op den Camp,H.J., van der Drift,C., Cirpus,I., van de Pas-Schoonen,K.T., Harhangi,H.R., van Niftrik,L., Schmid,M., Keltjens,J., van de Vossenberg,J., Kartal,B., Meier,H., Frishman,D., Huynen,M.A., Mewes,H.W., Weissenbach,J., Jetten,M.S., Wagner,M. and Le Paslier,D.
(2006) Nature 440 (7085), 790-794