ISNieu1
- Family IS1595
- Group IS1595
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAJE01000028 | ND | Nitrosomonas eutropha | Nitrosomonas eutropha |
DNA section
IS Length : 920 bp
Ends
IR Length : 7/10
IRL : CCTGCTCTGCTCACATGAGCCATTTTCATTCTTCAAGCCCACGATTTTGT
IRR : CCTGATTAGCAACGAACGTCAGCCATTCGAATAAAGTGCAAGGGCTGCGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
CCTGCTCTGCTCACATGAGCCATTTTCATTCTTCAAGCCCACGATTTTGTAAGTAATCGTTCAGGATGCGACAAGCGCTGGAAGTTGCCCGTTGGTCGGA
TGGGTTTCACTGTCCACGCTGTAACGGTACTGCTTACTATGTCATACGTGATGGTATACGCAAGGTTTTTCAATGCAGCGCCTGCCGACATCAGACCTCA
TTGATTGCCGGGACAGTTTTTCAGGGCACTAAACTACCATTGACTGTCTGGTTTTTGGCAATTTATCCGGTTAGTCAGGCCAAGACAGGTTTGTCGTCCC
TGGCGCTCAAACGCCAGCTGGGAGTGAGCTATCCTACGGCAGGGTTGATACACCACAAACTGATGCAGGCGATGGCTAATCGTGAGGAGCGTTATGTCCT
TGATGGCAGAATCCAGGTCGATGATGTTTATCTTGGTGGAGAACGTGCGGGAGGAAAAACGGGCAGAGGCTCAGAGAATAAGGTGCCTATTGTTGCCGCT
GTGTCTCTGACTGAGGATGATCGCCCTTTGCGCATGAGGCTTACCCCAGTCTCAACCTGGGTAAAGGATTGTCTGGCACCCGGCAGCACGGTGTTCTCGG
ATGCCTCGGCCTGCTGGTGCCGTTACCAAAATGGGCTGTAACCACCATTTTACAGTTATCGCCGGACGCAAACCAAAGGAGACTCCCGAGTTCTGGTGGA
TCAACACTATCCTCGGCAATCTCAAGACCAGCTTGTCGGGTTGTTACCATGCTTTCAACTTCCGCAAGTATGCCGCTCGCTATCTTGCCACCTTCAGTTA
CCGATTCAACCGGCGTTTTGATCTTTGCTCACTGCACGAAAAATTGCTGATCGCCGCTGCTTCGACCACTCCGCAGCCCTTGCACTTTATTCGAATGGCT
GACGTTCGTTGCTAATCAGG
TGGGTTTCACTGTCCACGCTGTAACGGTACTGCTTACTATGTCATACGTGATGGTATACGCAAGGTTTTTCAATGCAGCGCCTGCCGACATCAGACCTCA
TTGATTGCCGGGACAGTTTTTCAGGGCACTAAACTACCATTGACTGTCTGGTTTTTGGCAATTTATCCGGTTAGTCAGGCCAAGACAGGTTTGTCGTCCC
TGGCGCTCAAACGCCAGCTGGGAGTGAGCTATCCTACGGCAGGGTTGATACACCACAAACTGATGCAGGCGATGGCTAATCGTGAGGAGCGTTATGTCCT
TGATGGCAGAATCCAGGTCGATGATGTTTATCTTGGTGGAGAACGTGCGGGAGGAAAAACGGGCAGAGGCTCAGAGAATAAGGTGCCTATTGTTGCCGCT
GTGTCTCTGACTGAGGATGATCGCCCTTTGCGCATGAGGCTTACCCCAGTCTCAACCTGGGTAAAGGATTGTCTGGCACCCGGCAGCACGGTGTTCTCGG
ATGCCTCGGCCTGCTGGTGCCGTTACCAAAATGGGCTGTAACCACCATTTTACAGTTATCGCCGGACGCAAACCAAAGGAGACTCCCGAGTTCTGGTGGA
TCAACACTATCCTCGGCAATCTCAAGACCAGCTTGTCGGGTTGTTACCATGCTTTCAACTTCCGCAAGTATGCCGCTCGCTATCTTGCCACCTTCAGTTA
CCGATTCAACCGGCGTTTTGATCTTTGCTCACTGCACGAAAAATTGCTGATCGCCGCTGCTTCGACCACTCCGCAGCCCTTGCACTTTATTCGAATGGCT
GACGTTCGTTGCTAATCAGG
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 850 bp | 274 aa | 66 | 915 | + | No |
Chemistry : DDE
ORF sequence :
MRQALEVARWSDGFHCPRCNGTAYYVIRDGIRKVFQCSACRHQTSLIAGTVFQGTKLPLTVWFLAIYPVSQAKTGLSSLALKRQLGVSYPTAGLIHHKLM
QAMANREERYVLDGRIQVDDVYLGGERAGGKTGRGSENKVPIVAAVSLTEDDRPLRMRLTPVSTWVKDCLAPGSTVFSDASACWCRYQNGLGRKPKETPE
FWWINTILGNLKTSLSGCYHAFNFRKYAARYLATFSYRFNRRFDLCSLHEKLLIAAASTTPQPLHFIRMADVRC
QAMANREERYVLDGRIQVDDVYLGGERAGGKTGRGSENKVPIVAAVSLTEDDRPLRMRLTPVSTWVKDCLAPGSTVFSDASACWCRYQNGLGRKPKETPE
FWWINTILGNLKTSLSGCYHAFNFRKYAARYLATFSYRFNRRFDLCSLHEKLLIAAASTTPQPLHFIRMADVRC
Blast result :
Comments
ISNieu1 is 60% aa similar to ISKpn3.
The transposase is reconstructed in silico.
The transposase is reconstructed in silico.
References
1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S. and Richardson,P. (2005) Direct submission GenBank.